In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. Leaves
Crude extract and fractions of Buddleja thyrsoides were investigated regarding antioxidant activities by DPPH, total phenolic contents by Folin-Ciocalteau and antimicrobial activity by the broth microdilution method. Total phenolics varied from 214.07 ± 3.6 to 438.4 ± 0.3 mg g-1. Crude extract, ethy...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2009-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000200002 |
| _version_ | 1811255839799902208 |
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| author | Janaina Dorneles Mahlke Aline Augusti Boligon Michel Mansur Machado Tatiana Borba Spader Sydney Hartz Alves Thais do Canto-Dorow Margareth Linde Athayde |
| author_facet | Janaina Dorneles Mahlke Aline Augusti Boligon Michel Mansur Machado Tatiana Borba Spader Sydney Hartz Alves Thais do Canto-Dorow Margareth Linde Athayde |
| author_sort | Janaina Dorneles Mahlke |
| collection | DOAJ |
| description | Crude extract and fractions of Buddleja thyrsoides were investigated regarding antioxidant activities by DPPH, total phenolic contents by Folin-Ciocalteau and antimicrobial activity by the broth microdilution method. Total phenolics varied from 214.07 ± 3.6 to 438.4 ± 0.3 mg g-1. Crude extract, ethyl acetate, dichloromethane and butanolic fractions exhibited a weak scavenging activity (SC50=186.04 ± 10.8, 137.70 ± 8.5, 146.89 ± 9.0 and 165.71 ± 3.2 µg mL-1, respectively). A correlation between the antioxidant activities and total phenolic contents could be shown (r=0.857, p<0.01). The lowest value of MIC was observed with butanolic fraction against Saccharomyces cerevisiae (MIC and MFC at 62.5 µg mL-1). Dichloromethane and ethyl acetate fractions were effective against Staphylococcus aureus with MIC value at 250 and 500 µg mL-1 respectively. |
| first_indexed | 2024-04-12T17:30:03Z |
| format | Article |
| id | doaj.art-4432ceb6d1c849859b193b4f6fe29656 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-04-12T17:30:03Z |
| publishDate | 2009-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-4432ceb6d1c849859b193b4f6fe296562022-12-22T03:23:09ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642009-01-0132227728110.1590/S0100-40422009000200002In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. LeavesJanaina Dorneles MahlkeAline Augusti BoligonMichel Mansur MachadoTatiana Borba SpaderSydney Hartz AlvesThais do Canto-DorowMargareth Linde AthaydeCrude extract and fractions of Buddleja thyrsoides were investigated regarding antioxidant activities by DPPH, total phenolic contents by Folin-Ciocalteau and antimicrobial activity by the broth microdilution method. Total phenolics varied from 214.07 ± 3.6 to 438.4 ± 0.3 mg g-1. Crude extract, ethyl acetate, dichloromethane and butanolic fractions exhibited a weak scavenging activity (SC50=186.04 ± 10.8, 137.70 ± 8.5, 146.89 ± 9.0 and 165.71 ± 3.2 µg mL-1, respectively). A correlation between the antioxidant activities and total phenolic contents could be shown (r=0.857, p<0.01). The lowest value of MIC was observed with butanolic fraction against Saccharomyces cerevisiae (MIC and MFC at 62.5 µg mL-1). Dichloromethane and ethyl acetate fractions were effective against Staphylococcus aureus with MIC value at 250 and 500 µg mL-1 respectively.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000200002Buddleja thyrsoidesantimicrobialantioxidant activities |
| spellingShingle | Janaina Dorneles Mahlke Aline Augusti Boligon Michel Mansur Machado Tatiana Borba Spader Sydney Hartz Alves Thais do Canto-Dorow Margareth Linde Athayde In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. Leaves Química Nova Buddleja thyrsoides antimicrobial antioxidant activities |
| title | In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. Leaves |
| title_full | In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. Leaves |
| title_fullStr | In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. Leaves |
| title_full_unstemmed | In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. Leaves |
| title_short | In vitro antimicrobial and antioxidant activities of a crude extract and fractions from Buddleja thyrsoides Lam. Leaves |
| title_sort | in vitro antimicrobial and antioxidant activities of a crude extract and fractions from buddleja thyrsoides lam leaves |
| topic | Buddleja thyrsoides antimicrobial antioxidant activities |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000200002 |
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