Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features
The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to genera...
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MDPI AG
2019-01-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/9/1/120 |
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| author | Ana Agustina Aldana Laura Malatto Muhammad Atiq Ur Rehman Aldo Roberto Boccaccini Gustavo Abel Abraham |
| author_facet | Ana Agustina Aldana Laura Malatto Muhammad Atiq Ur Rehman Aldo Roberto Boccaccini Gustavo Abel Abraham |
| author_sort | Ana Agustina Aldana |
| collection | DOAJ |
| description | The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering. |
| first_indexed | 2024-12-21T06:42:53Z |
| format | Article |
| id | doaj.art-4433a5dd60f8481dac9fcff22a9fc160 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-21T06:42:53Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-4433a5dd60f8481dac9fcff22a9fc1602022-12-21T19:12:39ZengMDPI AGNanomaterials2079-49912019-01-019112010.3390/nano9010120nano9010120Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological FeaturesAna Agustina Aldana0Laura Malatto1Muhammad Atiq Ur Rehman2Aldo Roberto Boccaccini3Gustavo Abel Abraham4Instituto de Investigaciones en Ciencia y Tecnología de Materiales, INTEMA (UNMdP-CONICET), Av. Juan B. Justo 4302, Mar del Plata B7608FDQ, Buenos Aires, ArgentinaInstituto Nacional de Tecnología Industrial, Centro de Micro y Nanoelectrónica del Bicentenario (INTI-CMNB), Av. Gral. Paz 5445, San Martin B1650KNA, Buenos Aires, ArgentinaInstitute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, GermanyInstitute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, GermanyInstituto de Investigaciones en Ciencia y Tecnología de Materiales, INTEMA (UNMdP-CONICET), Av. Juan B. Justo 4302, Mar del Plata B7608FDQ, Buenos Aires, ArgentinaThe design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering.http://www.mdpi.com/2079-4991/9/1/120biomimetic scaffoldsgelatinelectrospinningmicromoldingbiomaterials |
| spellingShingle | Ana Agustina Aldana Laura Malatto Muhammad Atiq Ur Rehman Aldo Roberto Boccaccini Gustavo Abel Abraham Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features Nanomaterials biomimetic scaffolds gelatin electrospinning micromolding biomaterials |
| title | Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features |
| title_full | Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features |
| title_fullStr | Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features |
| title_full_unstemmed | Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features |
| title_short | Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features |
| title_sort | fabrication of gelatin methacrylate gelma scaffolds with nano and micro topographical and morphological features |
| topic | biomimetic scaffolds gelatin electrospinning micromolding biomaterials |
| url | http://www.mdpi.com/2079-4991/9/1/120 |
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