The impact of different diets and genders on fecal microbiota in Hanwoo cattle
Bovine fecal microbiota is important for host health and its composition can be affected by various factors, such as diet, age, species, breed, regions, and environments. The objective of this study was to evaluate the impact of diet and gender on fecal microbiota in Korean native Hanwoo cattle. The...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2022-09-01
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| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e71 |
| _version_ | 1811237363522732032 |
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| author | Seunghyeun Sim Huseong Lee Sang Yoon Hyeonsu Seon Cheolju Park Minseok Kim |
| author_facet | Seunghyeun Sim Huseong Lee Sang Yoon Hyeonsu Seon Cheolju Park Minseok Kim |
| author_sort | Seunghyeun Sim |
| collection | DOAJ |
| description | Bovine fecal microbiota is important for host health and its composition can be affected by various factors, such as diet, age, species, breed, regions, and environments. The objective of this study was to evaluate the impact of diet and gender on fecal microbiota in Korean native Hanwoo cattle. The 16S rRNA gene amplicon sequencing of fecal microbiota was conducted from 44 Hanwoo cattle divided into four groups: (1) 11 heifers fed an oat hay plus total mixed ration (TMR) diet for breeding (HOTB), (2) 11 heifers fed an early fattening TMR diet (HEFT), (3) 11 steers fed the early fattening TMR diet (SEFT), and (4) 11 steers fed the late fattening TMR diet (SLFT). Firmicutes and Bacteroidota were the first and second most dominant phyla in all the samples, respectively. The Firmicutes/Bacteroidota (F/B) ratio associated with feed efficiency was significantly greater in the SLFT group than in the other groups. At the genus level, Romboutsia, Paeniclostridium, and Turicibacterwere the most abundant in the SLFT while Akkermansia, Bacteroides, and Monoglobus were the most abundant in the HOTB group. Although the same early fattening TMR diet was fed to Hanwoo heifers and steers, Marvinbryantia and Coprococcus were the most abundant in the HEFT group while Alistipes and Ruminococcus were the most abundant in the SEFT group. Shannon and Simpson diversity indices were significantly lower in the SLFT group than in the other groups. Distribution of fecal microbiota and functional genetic profiles were significantly different among the four treatment groups. The present study demonstrates that different diets and genders can affect fecal microbiota and the F/B ratio may be associated with feed efficiency in Hanwoo cattle. Our results may help develop strategies to improve gut health and productivity through manipulation of fecal microbiota using the appropriate diet considering Hanwoo cattle gender. |
| first_indexed | 2024-04-12T12:23:08Z |
| format | Article |
| id | doaj.art-4434416e26d34022a92180a2f2675084 |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-04-12T12:23:08Z |
| publishDate | 2022-09-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-4434416e26d34022a92180a2f26750842022-12-22T03:33:14ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912022-09-0164589791010.5187/jast.2022.e71The impact of different diets and genders on fecal microbiota in Hanwoo cattleSeunghyeun Sim0Huseong Lee1Sang Yoon2Hyeonsu Seon3Cheolju Park4Minseok Kim5Division of Animal Science, Chonnam National University, Gwangju 61186, KoreaDivision of Animal Science, Chonnam National University, Gwangju 61186, KoreaDivision of Animal Science, Chonnam National University, Gwangju 61186, KoreaDivision of Animal Science, Chonnam National University, Gwangju 61186, KoreaDivision of Animal Science, Chonnam National University, Gwangju 61186, KoreaDivision of Animal Science, Chonnam National University, Gwangju 61186, KoreaBovine fecal microbiota is important for host health and its composition can be affected by various factors, such as diet, age, species, breed, regions, and environments. The objective of this study was to evaluate the impact of diet and gender on fecal microbiota in Korean native Hanwoo cattle. The 16S rRNA gene amplicon sequencing of fecal microbiota was conducted from 44 Hanwoo cattle divided into four groups: (1) 11 heifers fed an oat hay plus total mixed ration (TMR) diet for breeding (HOTB), (2) 11 heifers fed an early fattening TMR diet (HEFT), (3) 11 steers fed the early fattening TMR diet (SEFT), and (4) 11 steers fed the late fattening TMR diet (SLFT). Firmicutes and Bacteroidota were the first and second most dominant phyla in all the samples, respectively. The Firmicutes/Bacteroidota (F/B) ratio associated with feed efficiency was significantly greater in the SLFT group than in the other groups. At the genus level, Romboutsia, Paeniclostridium, and Turicibacterwere the most abundant in the SLFT while Akkermansia, Bacteroides, and Monoglobus were the most abundant in the HOTB group. Although the same early fattening TMR diet was fed to Hanwoo heifers and steers, Marvinbryantia and Coprococcus were the most abundant in the HEFT group while Alistipes and Ruminococcus were the most abundant in the SEFT group. Shannon and Simpson diversity indices were significantly lower in the SLFT group than in the other groups. Distribution of fecal microbiota and functional genetic profiles were significantly different among the four treatment groups. The present study demonstrates that different diets and genders can affect fecal microbiota and the F/B ratio may be associated with feed efficiency in Hanwoo cattle. Our results may help develop strategies to improve gut health and productivity through manipulation of fecal microbiota using the appropriate diet considering Hanwoo cattle gender. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e7116S rRNA gene amplicon sequencingDietFecal microbiotaGenderHanwoo |
| spellingShingle | Seunghyeun Sim Huseong Lee Sang Yoon Hyeonsu Seon Cheolju Park Minseok Kim The impact of different diets and genders on fecal microbiota in Hanwoo cattle Journal of Animal Science and Technology 16S rRNA gene amplicon sequencing Diet Fecal microbiota Gender Hanwoo |
| title | The impact of different diets and genders on fecal microbiota in Hanwoo cattle |
| title_full | The impact of different diets and genders on fecal microbiota in Hanwoo cattle |
| title_fullStr | The impact of different diets and genders on fecal microbiota in Hanwoo cattle |
| title_full_unstemmed | The impact of different diets and genders on fecal microbiota in Hanwoo cattle |
| title_short | The impact of different diets and genders on fecal microbiota in Hanwoo cattle |
| title_sort | impact of different diets and genders on fecal microbiota in hanwoo cattle |
| topic | 16S rRNA gene amplicon sequencing Diet Fecal microbiota Gender Hanwoo |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e71 |
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