A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm Maize
Genes at two unlinked loci (Tox1A and Tox1B) are required for production of the polyketide T-toxin by Cochliobolus heterostrophus race T, a pathogenic fungus that requires T-toxin for high virulence to maize with T-cytoplasm. Previous work indicated that Tox1A encodes a polyketide synthase (PKS1) re...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2002-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.9.883 |
| _version_ | 1818669099312480256 |
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| author | Mark S. Rose Sung-Hwan Yun Thipa Asvarak Shun-Wen Lu O. C. Yoder B. Gillian Turgeon |
| author_facet | Mark S. Rose Sung-Hwan Yun Thipa Asvarak Shun-Wen Lu O. C. Yoder B. Gillian Turgeon |
| author_sort | Mark S. Rose |
| collection | DOAJ |
| description | Genes at two unlinked loci (Tox1A and Tox1B) are required for production of the polyketide T-toxin by Cochliobolus heterostrophus race T, a pathogenic fungus that requires T-toxin for high virulence to maize with T-cytoplasm. Previous work indicated that Tox1A encodes a polyketide synthase (PKS1) required for T-toxin biosynthesis and for high virulence. To identify genes at Tox1B, a wild-type race T cDNA library was screened for genes missing in the genome of a Tox1B deletion mutant. The library was probed, first with a 415-kb NotI restriction fragment from the genome of the Tox1B¯ mutant, then with the corresponding 560-kb fragment from the genome of wild type. Two genes, DEC1 (similar to aceto-acetate decarboxylase-encoding genes) and RED1 (similar to genes encoding members of the medium-chain dehydro-genase/reductase superfamily), were recovered. Targeted disruption of DEC1 drastically reduced both T-toxin production and virulence of race T to T-cytoplasm maize, whereas specific inactivation of RED1 had no apparent effect on T-toxin production (as determined by bioassay) or on virulence. DEC1 and RED1 map within 1.5 kb of each other on Tox1B chromosome 6;12 and are unique to the genome of race T, an observation consistent with the hypothesis that these genes were acquired by C. heterostrophus via a horizontal transfer event. |
| first_indexed | 2024-12-17T06:46:50Z |
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| id | doaj.art-4434c633bafa4628be657c83279a70de |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T06:46:50Z |
| publishDate | 2002-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-4434c633bafa4628be657c83279a70de2022-12-21T21:59:43ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062002-09-0115988389310.1094/MPMI.2002.15.9.883A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm MaizeMark S. RoseSung-Hwan YunThipa AsvarakShun-Wen LuO. C. YoderB. Gillian TurgeonGenes at two unlinked loci (Tox1A and Tox1B) are required for production of the polyketide T-toxin by Cochliobolus heterostrophus race T, a pathogenic fungus that requires T-toxin for high virulence to maize with T-cytoplasm. Previous work indicated that Tox1A encodes a polyketide synthase (PKS1) required for T-toxin biosynthesis and for high virulence. To identify genes at Tox1B, a wild-type race T cDNA library was screened for genes missing in the genome of a Tox1B deletion mutant. The library was probed, first with a 415-kb NotI restriction fragment from the genome of the Tox1B¯ mutant, then with the corresponding 560-kb fragment from the genome of wild type. Two genes, DEC1 (similar to aceto-acetate decarboxylase-encoding genes) and RED1 (similar to genes encoding members of the medium-chain dehydro-genase/reductase superfamily), were recovered. Targeted disruption of DEC1 drastically reduced both T-toxin production and virulence of race T to T-cytoplasm maize, whereas specific inactivation of RED1 had no apparent effect on T-toxin production (as determined by bioassay) or on virulence. DEC1 and RED1 map within 1.5 kb of each other on Tox1B chromosome 6;12 and are unique to the genome of race T, an observation consistent with the hypothesis that these genes were acquired by C. heterostrophus via a horizontal transfer event.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.9.883Mycosphaerella zeae-maydisrace O |
| spellingShingle | Mark S. Rose Sung-Hwan Yun Thipa Asvarak Shun-Wen Lu O. C. Yoder B. Gillian Turgeon A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm Maize Molecular Plant-Microbe Interactions Mycosphaerella zeae-maydis race O |
| title | A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm Maize |
| title_full | A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm Maize |
| title_fullStr | A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm Maize |
| title_full_unstemmed | A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm Maize |
| title_short | A Decarboxylase Encoded at the Cochliobolus heterostrophus Translocation-Associated Tox1B Locus Is Required for Polyketide (T-toxin) Biosynthesis and High Virulence on T-cytoplasm Maize |
| title_sort | decarboxylase encoded at the cochliobolus heterostrophus translocation associated tox1b locus is required for polyketide t toxin biosynthesis and high virulence on t cytoplasm maize |
| topic | Mycosphaerella zeae-maydis race O |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.9.883 |
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