Porcine Protein Hydrolysates (PEPTEIVA<sup>®</sup>) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (<i>Sparus aurata</i>)
The effects of porcine plasma protein hydrolysate (PPH) on growth, feed efficiency, and immune responses was evaluated in <i>Sparus aurata</i>. Fish were fed two isoproteic (48% protein), isolipidic (17% fat), and isoenergetic diets (21.7 MJ/kg) diets, one of them containing 5% PPH at th...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/7/2122 |
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| author | Enric Gisbert Antoni Ibarz Joana P. Firmino Laura Fernández-Alacid Ricardo Salomón Eva Vallejos-Vidal Alberto Ruiz Javier Polo Ignasi Sanahuja Felipe E. Reyes-López Lluis Tort Karl B. Andree |
| author_facet | Enric Gisbert Antoni Ibarz Joana P. Firmino Laura Fernández-Alacid Ricardo Salomón Eva Vallejos-Vidal Alberto Ruiz Javier Polo Ignasi Sanahuja Felipe E. Reyes-López Lluis Tort Karl B. Andree |
| author_sort | Enric Gisbert |
| collection | DOAJ |
| description | The effects of porcine plasma protein hydrolysate (PPH) on growth, feed efficiency, and immune responses was evaluated in <i>Sparus aurata</i>. Fish were fed two isoproteic (48% protein), isolipidic (17% fat), and isoenergetic diets (21.7 MJ/kg) diets, one of them containing 5% PPH at the expense of fishmeal. Both diets were tested for 92 days. A significant increase in growth was observed in fish fed the PPH diet in comparison to the control group (182.2 ± 4.4 vs. 173.8 ± 4.1 g), as well as an increase in feed intake without worsening FCR values. An ex vivo assay, with splenocytes incubated with lipopolysaccharide, was conducted to evaluate the cellular immune competence of fish. Genes involved in humoral immunity <i>(lys</i>, <i>IgM</i>), pro- (<i>tnf-α</i>, <i>il-1β</i>), and anti-inflammatory (<i>tgf-β1</i>, <i>il10</i>) cytokines were upregulated in the PPH group in comparison to the control group. The inclusion of PPH in diets enhanced the antibacterial capacity of skin mucus, as the co-culture of selected bacteria <i>(E. coli</i>, <i>V. anguillarum</i>, and <i>P. anguilliseptica</i>) with skin mucus indicated. The present results showed that the PPH in low fishmeal diets (2%) promoted growth and feed efficiency, as well as enhancing the immune response, which indicates that this is a safe and functional ingredient for aquafeeds. |
| first_indexed | 2024-03-10T09:47:48Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:47:48Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-44390cdbce7940898c00ae6941edbf9b2023-11-22T03:02:46ZengMDPI AGAnimals2076-26152021-07-01117212210.3390/ani11072122Porcine Protein Hydrolysates (PEPTEIVA<sup>®</sup>) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (<i>Sparus aurata</i>)Enric Gisbert0Antoni Ibarz1Joana P. Firmino2Laura Fernández-Alacid3Ricardo Salomón4Eva Vallejos-Vidal5Alberto Ruiz6Javier Polo7Ignasi Sanahuja8Felipe E. Reyes-López9Lluis Tort10Karl B. Andree11IRTA, Centre de Sant Carles de la Ràpita, Aquaculture Program, Carretera Poble Nou, km 5.5, 43540 Sant Carles de la Ràpita, SpainDepartment of Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, Avda. Diagonal 643, 08028 Barcelona, SpainIRTA, Centre de Sant Carles de la Ràpita, Aquaculture Program, Carretera Poble Nou, km 5.5, 43540 Sant Carles de la Ràpita, SpainDepartment of Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, Avda. Diagonal 643, 08028 Barcelona, SpainIRTA, Centre de Sant Carles de la Ràpita, Aquaculture Program, Carretera Poble Nou, km 5.5, 43540 Sant Carles de la Ràpita, SpainCentro de Biotecnología Acuícola, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago 9170002, ChileIRTA, Centre de Sant Carles de la Ràpita, Aquaculture Program, Carretera Poble Nou, km 5.5, 43540 Sant Carles de la Ràpita, SpainAPC Europe SL, Avda. Sant Julià 246-258, 08403 Granollers, SpainDepartment of Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, Avda. Diagonal 643, 08028 Barcelona, SpainCentro de Biotecnología Acuícola, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago 9170002, ChileDepartment of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainIRTA, Centre de Sant Carles de la Ràpita, Aquaculture Program, Carretera Poble Nou, km 5.5, 43540 Sant Carles de la Ràpita, SpainThe effects of porcine plasma protein hydrolysate (PPH) on growth, feed efficiency, and immune responses was evaluated in <i>Sparus aurata</i>. Fish were fed two isoproteic (48% protein), isolipidic (17% fat), and isoenergetic diets (21.7 MJ/kg) diets, one of them containing 5% PPH at the expense of fishmeal. Both diets were tested for 92 days. A significant increase in growth was observed in fish fed the PPH diet in comparison to the control group (182.2 ± 4.4 vs. 173.8 ± 4.1 g), as well as an increase in feed intake without worsening FCR values. An ex vivo assay, with splenocytes incubated with lipopolysaccharide, was conducted to evaluate the cellular immune competence of fish. Genes involved in humoral immunity <i>(lys</i>, <i>IgM</i>), pro- (<i>tnf-α</i>, <i>il-1β</i>), and anti-inflammatory (<i>tgf-β1</i>, <i>il10</i>) cytokines were upregulated in the PPH group in comparison to the control group. The inclusion of PPH in diets enhanced the antibacterial capacity of skin mucus, as the co-culture of selected bacteria <i>(E. coli</i>, <i>V. anguillarum</i>, and <i>P. anguilliseptica</i>) with skin mucus indicated. The present results showed that the PPH in low fishmeal diets (2%) promoted growth and feed efficiency, as well as enhancing the immune response, which indicates that this is a safe and functional ingredient for aquafeeds.https://www.mdpi.com/2076-2615/11/7/2122aquaculturefunctional feedprotein hydrolysateslow fishmeal dietpathogen-associated molecular pattern (PAMP)skin-associated lymphoid tissue (SALT) |
| spellingShingle | Enric Gisbert Antoni Ibarz Joana P. Firmino Laura Fernández-Alacid Ricardo Salomón Eva Vallejos-Vidal Alberto Ruiz Javier Polo Ignasi Sanahuja Felipe E. Reyes-López Lluis Tort Karl B. Andree Porcine Protein Hydrolysates (PEPTEIVA<sup>®</sup>) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (<i>Sparus aurata</i>) Animals aquaculture functional feed protein hydrolysates low fishmeal diet pathogen-associated molecular pattern (PAMP) skin-associated lymphoid tissue (SALT) |
| title | Porcine Protein Hydrolysates (PEPTEIVA<sup>®</sup>) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_full | Porcine Protein Hydrolysates (PEPTEIVA<sup>®</sup>) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_fullStr | Porcine Protein Hydrolysates (PEPTEIVA<sup>®</sup>) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_full_unstemmed | Porcine Protein Hydrolysates (PEPTEIVA<sup>®</sup>) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_short | Porcine Protein Hydrolysates (PEPTEIVA<sup>®</sup>) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_sort | porcine protein hydrolysates pepteiva sup r sup promote growth and enhance systemic immunity in gilthead sea bream i sparus aurata i |
| topic | aquaculture functional feed protein hydrolysates low fishmeal diet pathogen-associated molecular pattern (PAMP) skin-associated lymphoid tissue (SALT) |
| url | https://www.mdpi.com/2076-2615/11/7/2122 |
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