Studies of phytochemical constituents by UPLC-QTOF-MS/MS of black hulless barley bran and its antioxidation and α-glucosidase inhibition effect
Anthocyanidins and flavonoids are important components in plant. In present study, the anthocyanidins and flavonoids in black hulless barley bran were identified by operating UPLC-QTOF-MS/MS. Among them, chrysoeriol 7-O-glucouronid, luteolin 7-O-glucouronid, chrysoeriol, and luteolin were quantified...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535224000467 |
| _version_ | 1797311572487438336 |
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| author | Junlin Deng Manyou Yu Yuehang Yang Tinghui Liu Zhuoya Xiang Jian Chen Kaijun Yang Ruiling Zhan Boyu Zhu Yongqing Zhu Xing Yang Yan Wan Chen Xia |
| author_facet | Junlin Deng Manyou Yu Yuehang Yang Tinghui Liu Zhuoya Xiang Jian Chen Kaijun Yang Ruiling Zhan Boyu Zhu Yongqing Zhu Xing Yang Yan Wan Chen Xia |
| author_sort | Junlin Deng |
| collection | DOAJ |
| description | Anthocyanidins and flavonoids are important components in plant. In present study, the anthocyanidins and flavonoids in black hulless barley bran were identified by operating UPLC-QTOF-MS/MS. Among them, chrysoeriol 7-O-glucouronid, luteolin 7-O-glucouronid, chrysoeriol, and luteolin were quantified by using UPLC. Additionally, the total phenolic content (TPC), total flavonoids content (TFC) and total anthocyanin content (TAC) were determined. Furthermore, the antioxidant activity (via ferric reducing, DPPH and ABTS method) and α-glucosidase inhibition effect were estimated. From the results, fifty-one constituents comprised 19 anthocyanidins and 32 flavonoids were detected in hulless barley bran fractions. Wherein compounds 1 and 9–19 (belong to anthocyanidins), and 21, 25–32, 35–42, 44–50 (belong to flavonoids) were first identified in hulless barley. The contents of chrysoeriol 7-O-glucouronid luteolin 7-O-glucouronid, chrysoeriol and luteolin were higher in E80% than that of in E40%, but were not detected in E20%. Another, the TAC exhibited the trend of E40% > E80% > E20%, and the TPC and TFC showed same trend of E40% > E20% > E80%. The higher TPC and TFC showed stronger antioxidant activity. However, no significant correlation between TAC, TPC, TFC and α-glucosidase inhibitory activity was observed. These results provided the anthocyanidins and flavonoids information, antioxidant value and α-glucosidase inhibition potential, which could provide reference in the development of black hulless barley bran. |
| first_indexed | 2024-03-08T02:01:32Z |
| format | Article |
| id | doaj.art-443a5737d5d84742b519fd8c8c2989dd |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-08T02:01:32Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-443a5737d5d84742b519fd8c8c2989dd2024-02-14T05:16:16ZengElsevierArabian Journal of Chemistry1878-53522024-03-01173105644Studies of phytochemical constituents by UPLC-QTOF-MS/MS of black hulless barley bran and its antioxidation and α-glucosidase inhibition effectJunlin Deng0Manyou Yu1Yuehang Yang2Tinghui Liu3Zhuoya Xiang4Jian Chen5Kaijun Yang6Ruiling Zhan7Boyu Zhu8Yongqing Zhu9Xing Yang10Yan Wan11Chen Xia12Institute of Agro-Products Processing Science and Technology (Institute of Food Nutrition and Health), Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology (Institute of Food Nutrition and Health), Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSchool of Pharmacy and Bioengineering, Chengdu University, Chengdu 610106, ChinaGanzi Academy of Agricultural Sciences, Kangding 626000, ChinaInstitute of Agro-Products Processing Science and Technology (Institute of Food Nutrition and Health), Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology (Institute of Food Nutrition and Health), Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaGanzi Academy of Agricultural Sciences, Kangding 626000, ChinaGanzi Academy of Agricultural Sciences, Kangding 626000, ChinaInstitute of Agro-Products Processing Science and Technology (Institute of Food Nutrition and Health), Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology (Institute of Food Nutrition and Health), Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology (Institute of Food Nutrition and Health), Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSchool of Pharmacy and Bioengineering, Chengdu University, Chengdu 610106, China; Corresponding authors.Institute of Agro-Products Processing Science and Technology (Institute of Food Nutrition and Health), Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; Corresponding authors.Anthocyanidins and flavonoids are important components in plant. In present study, the anthocyanidins and flavonoids in black hulless barley bran were identified by operating UPLC-QTOF-MS/MS. Among them, chrysoeriol 7-O-glucouronid, luteolin 7-O-glucouronid, chrysoeriol, and luteolin were quantified by using UPLC. Additionally, the total phenolic content (TPC), total flavonoids content (TFC) and total anthocyanin content (TAC) were determined. Furthermore, the antioxidant activity (via ferric reducing, DPPH and ABTS method) and α-glucosidase inhibition effect were estimated. From the results, fifty-one constituents comprised 19 anthocyanidins and 32 flavonoids were detected in hulless barley bran fractions. Wherein compounds 1 and 9–19 (belong to anthocyanidins), and 21, 25–32, 35–42, 44–50 (belong to flavonoids) were first identified in hulless barley. The contents of chrysoeriol 7-O-glucouronid luteolin 7-O-glucouronid, chrysoeriol and luteolin were higher in E80% than that of in E40%, but were not detected in E20%. Another, the TAC exhibited the trend of E40% > E80% > E20%, and the TPC and TFC showed same trend of E40% > E20% > E80%. The higher TPC and TFC showed stronger antioxidant activity. However, no significant correlation between TAC, TPC, TFC and α-glucosidase inhibitory activity was observed. These results provided the anthocyanidins and flavonoids information, antioxidant value and α-glucosidase inhibition potential, which could provide reference in the development of black hulless barley bran.http://www.sciencedirect.com/science/article/pii/S1878535224000467Black hulless barley branPhytochemical profilingAntioxidant activityUPLC-QTOF-MS/MS |
| spellingShingle | Junlin Deng Manyou Yu Yuehang Yang Tinghui Liu Zhuoya Xiang Jian Chen Kaijun Yang Ruiling Zhan Boyu Zhu Yongqing Zhu Xing Yang Yan Wan Chen Xia Studies of phytochemical constituents by UPLC-QTOF-MS/MS of black hulless barley bran and its antioxidation and α-glucosidase inhibition effect Arabian Journal of Chemistry Black hulless barley bran Phytochemical profiling Antioxidant activity UPLC-QTOF-MS/MS |
| title | Studies of phytochemical constituents by UPLC-QTOF-MS/MS of black hulless barley bran and its antioxidation and α-glucosidase inhibition effect |
| title_full | Studies of phytochemical constituents by UPLC-QTOF-MS/MS of black hulless barley bran and its antioxidation and α-glucosidase inhibition effect |
| title_fullStr | Studies of phytochemical constituents by UPLC-QTOF-MS/MS of black hulless barley bran and its antioxidation and α-glucosidase inhibition effect |
| title_full_unstemmed | Studies of phytochemical constituents by UPLC-QTOF-MS/MS of black hulless barley bran and its antioxidation and α-glucosidase inhibition effect |
| title_short | Studies of phytochemical constituents by UPLC-QTOF-MS/MS of black hulless barley bran and its antioxidation and α-glucosidase inhibition effect |
| title_sort | studies of phytochemical constituents by uplc qtof ms ms of black hulless barley bran and its antioxidation and α glucosidase inhibition effect |
| topic | Black hulless barley bran Phytochemical profiling Antioxidant activity UPLC-QTOF-MS/MS |
| url | http://www.sciencedirect.com/science/article/pii/S1878535224000467 |
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