Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv.
A reversed-phase liquid chromatographic method was developed for the quantitative determination of three major hypotensive compounds, namely geniposidic acid, chlorogenic acid, and geniposide in the bark of Eucommia ulmoides. Soxhlet extraction of GPA, GPS, and CA from E. ulmoides was optimized acco...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2009-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640904811X.pdf |
| _version_ | 1818475573167521792 |
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| author | Xiao Jianbo Wei Xinlin Wang Yuanfeng |
| author_facet | Xiao Jianbo Wei Xinlin Wang Yuanfeng |
| author_sort | Xiao Jianbo |
| collection | DOAJ |
| description | A reversed-phase liquid chromatographic method was developed for the quantitative determination of three major hypotensive compounds, namely geniposidic acid, chlorogenic acid, and geniposide in the bark of Eucommia ulmoides. Soxhlet extraction of GPA, GPS, and CA from E. ulmoides was optimized according to the Taguchi experimental design. Maximum global yields were obtained using the following conditions: extraction temperature, 80°C; extraction time, 1 h; number of extractions, three; solvent volume, 16 ml/g of sample; and 50% ethanol concentration in water. Optimal conditions of separation and detection were achieved on a Diamonsil ODS C18 column (150 mm × 4.6 mm, 5 μm) with a linear gradient of methanol and 0.04% aqueous phosphoric acid (v/v) at a flow rate of 1.0 ml/min and detection wavelength of 240 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 3% for intra- and inter-day assays, and the recovery percentage of the method was 95-104%, with a relative standard deviation (R.S.D.) of less than 5%. The current assay method was used for quantitative determination of geniposidic acid, chlorogenic acid, and geniposide in five samples of E. ulmoides with different age. The results indicate that the developed method could be readily utilized as a quality control method in working with E. ulmoides. |
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| format | Article |
| id | doaj.art-443aec7ff3de49bb98b85256ce06bee9 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 |
| language | English |
| last_indexed | 2024-12-10T09:14:25Z |
| publishDate | 2009-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
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| series | Archives of Biological Sciences |
| spelling | doaj.art-443aec7ff3de49bb98b85256ce06bee92022-12-22T01:54:55ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642009-01-0161481181710.2298/ABS0904811XExtraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv.Xiao JianboWei XinlinWang YuanfengA reversed-phase liquid chromatographic method was developed for the quantitative determination of three major hypotensive compounds, namely geniposidic acid, chlorogenic acid, and geniposide in the bark of Eucommia ulmoides. Soxhlet extraction of GPA, GPS, and CA from E. ulmoides was optimized according to the Taguchi experimental design. Maximum global yields were obtained using the following conditions: extraction temperature, 80°C; extraction time, 1 h; number of extractions, three; solvent volume, 16 ml/g of sample; and 50% ethanol concentration in water. Optimal conditions of separation and detection were achieved on a Diamonsil ODS C18 column (150 mm × 4.6 mm, 5 μm) with a linear gradient of methanol and 0.04% aqueous phosphoric acid (v/v) at a flow rate of 1.0 ml/min and detection wavelength of 240 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 3% for intra- and inter-day assays, and the recovery percentage of the method was 95-104%, with a relative standard deviation (R.S.D.) of less than 5%. The current assay method was used for quantitative determination of geniposidic acid, chlorogenic acid, and geniposide in five samples of E. ulmoides with different age. The results indicate that the developed method could be readily utilized as a quality control method in working with E. ulmoides.http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640904811X.pdfEucommia ulmoidesgeniposidic acidchlorogenic acidgeniposideHPLCChina |
| spellingShingle | Xiao Jianbo Wei Xinlin Wang Yuanfeng Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv. Archives of Biological Sciences Eucommia ulmoides geniposidic acid chlorogenic acid geniposide HPLC China |
| title | Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv. |
| title_full | Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv. |
| title_fullStr | Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv. |
| title_full_unstemmed | Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv. |
| title_short | Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv. |
| title_sort | extraction and determination of major hypotensive compounds in bark of eucommia ulmoides oliv |
| topic | Eucommia ulmoides geniposidic acid chlorogenic acid geniposide HPLC China |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640904811X.pdf |
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