Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambs
The objective of this study was to identify Myostatin (MSTN) gene polymorphism using PCR RFLP technique in order to improve sheep meat evaluation. There are data which show that MSTN is an inhibitor of skeletal muscle growth and a mutation in gene leads to increased muscle mass. Therefore, it is con...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/421 |
| _version_ | 1797214809148620800 |
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| author | Cristina Lazar Al.M. Gras M.C. Rotar Gina Cecilia Pistol Rodica Stefania Pelmus Elena Ghiţă |
| author_facet | Cristina Lazar Al.M. Gras M.C. Rotar Gina Cecilia Pistol Rodica Stefania Pelmus Elena Ghiţă |
| author_sort | Cristina Lazar |
| collection | DOAJ |
| description | The objective of this study was to identify Myostatin (MSTN) gene polymorphism using PCR RFLP technique in order to improve sheep meat evaluation. There are data which show that MSTN is an inhibitor of skeletal muscle growth and a mutation in gene leads to increased muscle mass. Therefore, it is considered as an appropriate candidate gene for carcass meat quality. Blood samples were collected from 105 Teleorman Black Head (TBH) lambs and DNA was extracted using, Wizard Genomic DNA Purification Kit. Polymorphism of MSTN was determined by PCR amplification followed by RFLP method using restriction enzyme Hae III. Based on results, two genotypes mm and Mm were identified. It was identified homozygous genotype mm which not carry this mutation with 16.67% and heterozygous genotype Mm with 83.33%. M allele frequency was 42% and for allele m was 58%. Observed and expected values of MSTN genotypes were found in Hardy Weinberg disequilibrium, after χ2 test was calculated. MM individuals who carry this mutation were not identified in the analysed herd. This may be due to an empirical selection of this breed leading to absence of this genotype. That is why further investigation on a high number of animals are necessary to discover this mutation correlated with meat quality in TBH sheep. |
| first_indexed | 2024-04-24T11:20:04Z |
| format | Article |
| id | doaj.art-443d4a68eb2946aa86858f90a90ead5b |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:20:04Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-443d4a68eb2946aa86858f90a90ead5b2024-04-11T04:42:27ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-014916363421Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambsCristina Lazar0Al.M. Gras1M.C. Rotar2Gina Cecilia Pistol3Rodica Stefania Pelmus4Elena Ghiţă5National Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015-Balotesti, Calea Bucuresti, no. 1, RomaniaThe objective of this study was to identify Myostatin (MSTN) gene polymorphism using PCR RFLP technique in order to improve sheep meat evaluation. There are data which show that MSTN is an inhibitor of skeletal muscle growth and a mutation in gene leads to increased muscle mass. Therefore, it is considered as an appropriate candidate gene for carcass meat quality. Blood samples were collected from 105 Teleorman Black Head (TBH) lambs and DNA was extracted using, Wizard Genomic DNA Purification Kit. Polymorphism of MSTN was determined by PCR amplification followed by RFLP method using restriction enzyme Hae III. Based on results, two genotypes mm and Mm were identified. It was identified homozygous genotype mm which not carry this mutation with 16.67% and heterozygous genotype Mm with 83.33%. M allele frequency was 42% and for allele m was 58%. Observed and expected values of MSTN genotypes were found in Hardy Weinberg disequilibrium, after χ2 test was calculated. MM individuals who carry this mutation were not identified in the analysed herd. This may be due to an empirical selection of this breed leading to absence of this genotype. That is why further investigation on a high number of animals are necessary to discover this mutation correlated with meat quality in TBH sheep.https://spasb.ro/index.php/public_html/article/view/421myostatincarcasssheepmeatevaluationqualitypolymorphism |
| spellingShingle | Cristina Lazar Al.M. Gras M.C. Rotar Gina Cecilia Pistol Rodica Stefania Pelmus Elena Ghiţă Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambs Scientific Papers Animal Science and Biotechnologies myostatin carcass sheep meat evaluation quality polymorphism |
| title | Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambs |
| title_full | Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambs |
| title_fullStr | Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambs |
| title_full_unstemmed | Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambs |
| title_short | Identification of Myostatin gene polymorphism using PCR-RFLP for improving carcass meat evaluation of Teleorman Black Head lambs |
| title_sort | identification of myostatin gene polymorphism using pcr rflp for improving carcass meat evaluation of teleorman black head lambs |
| topic | myostatin carcass sheep meat evaluation quality polymorphism |
| url | https://spasb.ro/index.php/public_html/article/view/421 |
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