Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India
Sixteen ivy gourd (Coccinia grandis (L.) Voigt) plant samples showing severe mosaic symptoms were collected from New Delhi and Varanasi (Uttar Pradesh) in India. Begomovirus infection was confirmed by PCR using begomovirusspecific primers. Amplified PCR products (1.2 kb fragments) were cloned and th...
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| Format: | Article |
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University of Belgrade, University of Novi Sad
2018-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641700051V.pdf |
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| author | Venkataravanappa Venkataravanappa Narasimha Reddy Lakshminarayana Reddy C. Saha Sujoy Subbanna Shankarappa K. Manem Krishna Reddy |
| author_facet | Venkataravanappa Venkataravanappa Narasimha Reddy Lakshminarayana Reddy C. Saha Sujoy Subbanna Shankarappa K. Manem Krishna Reddy |
| author_sort | Venkataravanappa Venkataravanappa |
| collection | DOAJ |
| description | Sixteen ivy gourd (Coccinia grandis (L.) Voigt) plant samples showing severe mosaic symptoms were collected from New Delhi and Varanasi (Uttar Pradesh) in India. Begomovirus infection was confirmed by PCR using begomovirusspecific primers. Amplified PCR products (1.2 kb fragments) were cloned and the sequence was characterized. Based on sequence analysis, begomovirus associated with the majority of ivy gourd samples (16) was found to be a member of a bipartite begomovirus species, which is closely related to tomato leaf curl New Delhi virus (ToLCNDV). Therefore, two samples of ivy gourd, IVG1-ND and IVG2-Var, were selected for full-length genome (DNA-A and DNA-B-like sequence) amplification by the rolling circle DNA amplification (RCA) method. Sequence analysis performed using the Species Demarcation Tool (SDT) program revealed that they share 89.5-91.3% (IVG1-ND) and 93.4-96.8% (IVG2-Var) nucleotide (nt) identity with the DNA-A-like sequence of ToLCNDV isolated from cucurbits and chilli, respectively. The IVG1-ND and IVG2-Var isolates shared 90% nt identity among themselves, indicating that they are two different strains of ToLCNDV. Similarly, SDT analysis of the DNA-B-like sequence of IVG1-ND and IVG2-Var exhibited showed 82.7-93.3% nt identity with the DNA-B-like sequences of ToLCNDV infecting cucurbits. The recombination analysis of DNA-A and DNB-B-like sequences showed that the greater part of their genome most likely originated from previously reported begomoviruses that are known to infect chilli and cucurbits through recombination. |
| first_indexed | 2024-12-22T05:24:20Z |
| format | Article |
| id | doaj.art-443dfb0c644d400794126e6be9985e4e |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-22T05:24:20Z |
| publishDate | 2018-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
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| series | Archives of Biological Sciences |
| spelling | doaj.art-443dfb0c644d400794126e6be9985e4e2022-12-21T18:37:37ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392018-01-0170233934710.2298/ABS170616051V0354-46641700051VDetection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North IndiaVenkataravanappa Venkataravanappa0Narasimha Reddy Lakshminarayana Reddy C.1Saha Sujoy2Subbanna Shankarappa K.3Manem Krishna Reddy4Central Horticultural Experiment Station (CHES), Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore, IndiaUniversity of Agricultural Sciences (B), College of Sericulture, Department of Plant Pathology, Karnataka, IndiaNational Research Centre for Grapes, Division of Plant Pathology, Pune, Maharashtra, IndiaUniversity of Horticultural Sciences, College of Horticulture, Department of Plant Pathology, Bagalkot, Karnataka, IndiaIndian Institute of Horticultural Research, Hessaraghatta Lake PO, Karnataka, IndiaSixteen ivy gourd (Coccinia grandis (L.) Voigt) plant samples showing severe mosaic symptoms were collected from New Delhi and Varanasi (Uttar Pradesh) in India. Begomovirus infection was confirmed by PCR using begomovirusspecific primers. Amplified PCR products (1.2 kb fragments) were cloned and the sequence was characterized. Based on sequence analysis, begomovirus associated with the majority of ivy gourd samples (16) was found to be a member of a bipartite begomovirus species, which is closely related to tomato leaf curl New Delhi virus (ToLCNDV). Therefore, two samples of ivy gourd, IVG1-ND and IVG2-Var, were selected for full-length genome (DNA-A and DNA-B-like sequence) amplification by the rolling circle DNA amplification (RCA) method. Sequence analysis performed using the Species Demarcation Tool (SDT) program revealed that they share 89.5-91.3% (IVG1-ND) and 93.4-96.8% (IVG2-Var) nucleotide (nt) identity with the DNA-A-like sequence of ToLCNDV isolated from cucurbits and chilli, respectively. The IVG1-ND and IVG2-Var isolates shared 90% nt identity among themselves, indicating that they are two different strains of ToLCNDV. Similarly, SDT analysis of the DNA-B-like sequence of IVG1-ND and IVG2-Var exhibited showed 82.7-93.3% nt identity with the DNA-B-like sequences of ToLCNDV infecting cucurbits. The recombination analysis of DNA-A and DNB-B-like sequences showed that the greater part of their genome most likely originated from previously reported begomoviruses that are known to infect chilli and cucurbits through recombination.http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641700051V.pdfbegomovirusesivy gourdrecombinationphylogenetic analysisperennial crop |
| spellingShingle | Venkataravanappa Venkataravanappa Narasimha Reddy Lakshminarayana Reddy C. Saha Sujoy Subbanna Shankarappa K. Manem Krishna Reddy Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India Archives of Biological Sciences begomoviruses ivy gourd recombination phylogenetic analysis perennial crop |
| title | Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India |
| title_full | Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India |
| title_fullStr | Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India |
| title_full_unstemmed | Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India |
| title_short | Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India |
| title_sort | detection and characterization of tomato leaf curl new delhi virus association with mosaic disease of ivy gourd coccinia grandis l voigt in north india |
| topic | begomoviruses ivy gourd recombination phylogenetic analysis perennial crop |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641700051V.pdf |
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