Isolation and Molecular Identification of the Native Microflora on Flammulina velutipes Fruiting Bodies and Modeling the Growth of Dominant Microbiota (Lactococcus lactis)
The objectives of this study were to isolate and identify the dominant microorganism in Flammulina velutipes fruiting bodies (FVFB) and to develop kinetic models for describing its growth. The native microflora community on FVFB was isolated and identified using morphological examination and high-th...
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| Format: | Article |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.664874/full |
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| author | Qi Wei Qi Wei Qi Wei Xinyuan Pan Xinyuan Pan Jie Li Zhen Jia Ting Fang Yuji Jiang |
| author_facet | Qi Wei Qi Wei Qi Wei Xinyuan Pan Xinyuan Pan Jie Li Zhen Jia Ting Fang Yuji Jiang |
| author_sort | Qi Wei |
| collection | DOAJ |
| description | The objectives of this study were to isolate and identify the dominant microorganism in Flammulina velutipes fruiting bodies (FVFB) and to develop kinetic models for describing its growth. The native microflora community on FVFB was isolated and identified using morphological examination and high-throughput sequencing analysis. FVFB presented complex microbial communities with dominant microorganisms being Lactococcus lactis. Irradiated FVFB were inoculated with the isolated strain of L. lactis and cultivated at various temperatures (4, 10, 16, 20, 25, 32, and 37°C). Three primary models, namely the Huang, Baranyi and Roberts, and reparameterized Gompertz models, and three secondary models, namely the Huang square-root, Ratkowsky square-root, and Arrhenius-type models, were developed and evaluated. With the lowest values of mean square error (MSE, 0.023–0.161) and root mean square error (RMSE, 0.152–0.401) values, the reparameterized Gompertz model was more suitable to describe the growth of L. lactis on FVFB than both Huang and Baranyi and Roberts models. The Ratkowsky square-root model provided more accurate estimation for the effect of temperature on the specific growth rate of L. lactis. The minimum growth temperature predicted by the Ratkowsky square-root model was −7.1°C. The kinetic models developed in this study could be used to evaluate the growth behavior of L. lactis on FVFB and estimate the shelf-life of FVFB. |
| first_indexed | 2024-12-24T11:12:20Z |
| format | Article |
| id | doaj.art-443e0005608f4d369c65161b916d5b2c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-24T11:12:20Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-443e0005608f4d369c65161b916d5b2c2022-12-21T16:58:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-05-011210.3389/fmicb.2021.664874664874Isolation and Molecular Identification of the Native Microflora on Flammulina velutipes Fruiting Bodies and Modeling the Growth of Dominant Microbiota (Lactococcus lactis)Qi Wei0Qi Wei1Qi Wei2Xinyuan Pan3Xinyuan Pan4Jie Li5Zhen Jia6Ting Fang7Yuji Jiang8College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Life Science, Ningde Normal University, Ningde, ChinaFujian Higher Education Research Center for Local Biological Resources, Ningde, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Anjoy Food Co., Ltd., Xiamen, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaThe objectives of this study were to isolate and identify the dominant microorganism in Flammulina velutipes fruiting bodies (FVFB) and to develop kinetic models for describing its growth. The native microflora community on FVFB was isolated and identified using morphological examination and high-throughput sequencing analysis. FVFB presented complex microbial communities with dominant microorganisms being Lactococcus lactis. Irradiated FVFB were inoculated with the isolated strain of L. lactis and cultivated at various temperatures (4, 10, 16, 20, 25, 32, and 37°C). Three primary models, namely the Huang, Baranyi and Roberts, and reparameterized Gompertz models, and three secondary models, namely the Huang square-root, Ratkowsky square-root, and Arrhenius-type models, were developed and evaluated. With the lowest values of mean square error (MSE, 0.023–0.161) and root mean square error (RMSE, 0.152–0.401) values, the reparameterized Gompertz model was more suitable to describe the growth of L. lactis on FVFB than both Huang and Baranyi and Roberts models. The Ratkowsky square-root model provided more accurate estimation for the effect of temperature on the specific growth rate of L. lactis. The minimum growth temperature predicted by the Ratkowsky square-root model was −7.1°C. The kinetic models developed in this study could be used to evaluate the growth behavior of L. lactis on FVFB and estimate the shelf-life of FVFB.https://www.frontiersin.org/articles/10.3389/fmicb.2021.664874/fullFlammulina velutipesmicrofloramathematical modelLactococcus lactisgrowth |
| spellingShingle | Qi Wei Qi Wei Qi Wei Xinyuan Pan Xinyuan Pan Jie Li Zhen Jia Ting Fang Yuji Jiang Isolation and Molecular Identification of the Native Microflora on Flammulina velutipes Fruiting Bodies and Modeling the Growth of Dominant Microbiota (Lactococcus lactis) Frontiers in Microbiology Flammulina velutipes microflora mathematical model Lactococcus lactis growth |
| title | Isolation and Molecular Identification of the Native Microflora on Flammulina velutipes Fruiting Bodies and Modeling the Growth of Dominant Microbiota (Lactococcus lactis) |
| title_full | Isolation and Molecular Identification of the Native Microflora on Flammulina velutipes Fruiting Bodies and Modeling the Growth of Dominant Microbiota (Lactococcus lactis) |
| title_fullStr | Isolation and Molecular Identification of the Native Microflora on Flammulina velutipes Fruiting Bodies and Modeling the Growth of Dominant Microbiota (Lactococcus lactis) |
| title_full_unstemmed | Isolation and Molecular Identification of the Native Microflora on Flammulina velutipes Fruiting Bodies and Modeling the Growth of Dominant Microbiota (Lactococcus lactis) |
| title_short | Isolation and Molecular Identification of the Native Microflora on Flammulina velutipes Fruiting Bodies and Modeling the Growth of Dominant Microbiota (Lactococcus lactis) |
| title_sort | isolation and molecular identification of the native microflora on flammulina velutipes fruiting bodies and modeling the growth of dominant microbiota lactococcus lactis |
| topic | Flammulina velutipes microflora mathematical model Lactococcus lactis growth |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.664874/full |
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