Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts
The contribution of halophilic microorganisms to the quality of salts produced in solar salterns has recently been recognized but not clearly understood. Using metagenomic 16S rRNA gene sequencing approach, we showed the microbial composition difference between the Tuban crystallization pond (CP-Tu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2023-07-01
|
| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/40033 |
| _version_ | 1827905046139895808 |
|---|---|
| author | Ekowati Chasanah Asri Pratitis Rini Susilowati Agus Heri Purnomo Hedi I. Januar Agustinus R. Uria |
| author_facet | Ekowati Chasanah Asri Pratitis Rini Susilowati Agus Heri Purnomo Hedi I. Januar Agustinus R. Uria |
| author_sort | Ekowati Chasanah |
| collection | DOAJ |
| description |
The contribution of halophilic microorganisms to the quality of salts produced in solar salterns has recently been recognized but not clearly understood. Using metagenomic 16S rRNA gene sequencing approach, we showed the microbial composition difference between the Tuban crystallization pond (CP-Tuban) that produces low-quality salt and CP-Sampang as a representative solar saltern that yields high-quality salt. Dominant classes in both traditional salterns were represented by γ-proteobacteria and halobacteria that occurred at higher prevalence in CP-Sampang. Microbial taxa, including beneficial genera, in CP-Sampang were more diverse and abundant compared to CP-Tuban. Among 180 genus-level OTUs identified in CP-Sampang, 127 of them were considered unique due to their absence in CP-Tuban. Higher levels of dissolved oxygen (DO) and nutrient (phosphate, nitrate, and ammonia) in the seawater reservoir (SR) of Sampang may contribute to more diverse phytoplankton genera, which could support the growth of beneficial heterotrophic microbes that positively affect the salt quality of the CP-Sampang. Low number of Dunaleilla sp. in both CPs do not seem to influence the quality of salts produced. The outcome of these comparative studies provides new insights into the contribution of diverse microbial taxa in correlation with physico-chemical parameters and phytoplankton communities to the high quality of salts produced in traditional solar salterns. The presence of beneficial genera in the enriched microbial cultures could provide an important basis for further applications, such as improving the quality of salt produced and producing unique compounds and enzymes.
|
| first_indexed | 2024-03-13T00:36:37Z |
| format | Article |
| id | doaj.art-4441903fb48c40b3a6f532d8191bcefa |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-03-13T00:36:37Z |
| publishDate | 2023-07-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-4441903fb48c40b3a6f532d8191bcefa2023-07-10T02:23:48ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942023-07-0130510.4308/hjb.30.5.957-984Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality SaltsEkowati Chasanah0Asri Pratitis1Rini Susilowati2Agus Heri Purnomo3Hedi I. Januar4Agustinus R. Uria5Research Center for Marine and Inland Bioproducts,Organization Research (OR) Earth and Maritime,National Research and Innovation Agency (BRIN), North Lombok Regency, Province of West Nusa Tenggara 83352, IndonesiaDeputy STI Human Resources-BRIN, BJ Habibie Building, Central Jakarta 10340, IndonesiaDeputy STI Human Resources-BRIN, BJ Habibie Building, Central Jakarta 10340, IndonesiaResearch Center for Society and Culture-OR Social Sciences and Hummanities-BRIN, Jakarta 12710, IndonesiaResearch Center for Ecology and Ethnobiology, OR of Life Science and Environment, BRIN, KST Soekarno, Cibinong 16911, IndonesiaFaculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo 060-0812, Japan The contribution of halophilic microorganisms to the quality of salts produced in solar salterns has recently been recognized but not clearly understood. Using metagenomic 16S rRNA gene sequencing approach, we showed the microbial composition difference between the Tuban crystallization pond (CP-Tuban) that produces low-quality salt and CP-Sampang as a representative solar saltern that yields high-quality salt. Dominant classes in both traditional salterns were represented by γ-proteobacteria and halobacteria that occurred at higher prevalence in CP-Sampang. Microbial taxa, including beneficial genera, in CP-Sampang were more diverse and abundant compared to CP-Tuban. Among 180 genus-level OTUs identified in CP-Sampang, 127 of them were considered unique due to their absence in CP-Tuban. Higher levels of dissolved oxygen (DO) and nutrient (phosphate, nitrate, and ammonia) in the seawater reservoir (SR) of Sampang may contribute to more diverse phytoplankton genera, which could support the growth of beneficial heterotrophic microbes that positively affect the salt quality of the CP-Sampang. Low number of Dunaleilla sp. in both CPs do not seem to influence the quality of salts produced. The outcome of these comparative studies provides new insights into the contribution of diverse microbial taxa in correlation with physico-chemical parameters and phytoplankton communities to the high quality of salts produced in traditional solar salterns. The presence of beneficial genera in the enriched microbial cultures could provide an important basis for further applications, such as improving the quality of salt produced and producing unique compounds and enzymes. https://journal.ipb.ac.id/index.php/hayati/article/view/40033 |
| spellingShingle | Ekowati Chasanah Asri Pratitis Rini Susilowati Agus Heri Purnomo Hedi I. Januar Agustinus R. Uria Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts Hayati Journal of Biosciences |
| title | Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts |
| title_full | Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts |
| title_fullStr | Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts |
| title_full_unstemmed | Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts |
| title_short | Taxonomic Profiling of Microorganisms Inhabiting Two Solar Salterns that Produce High- and Low-Quality Salts |
| title_sort | taxonomic profiling of microorganisms inhabiting two solar salterns that produce high and low quality salts |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/40033 |
| work_keys_str_mv | AT ekowatichasanah taxonomicprofilingofmicroorganismsinhabitingtwosolarsalternsthatproducehighandlowqualitysalts AT asripratitis taxonomicprofilingofmicroorganismsinhabitingtwosolarsalternsthatproducehighandlowqualitysalts AT rinisusilowati taxonomicprofilingofmicroorganismsinhabitingtwosolarsalternsthatproducehighandlowqualitysalts AT agusheripurnomo taxonomicprofilingofmicroorganismsinhabitingtwosolarsalternsthatproducehighandlowqualitysalts AT hediijanuar taxonomicprofilingofmicroorganismsinhabitingtwosolarsalternsthatproducehighandlowqualitysalts AT agustinusruria taxonomicprofilingofmicroorganismsinhabitingtwosolarsalternsthatproducehighandlowqualitysalts |