Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana
The application of arbuscular mycorrhizal fungi (AM fungi) and phosphorus (P) can improve plant growth under drought stress by upregulating the antioxidant system and osmotic accumulation. The 14-3-3 protein can respond to different abiotic stresses such as low P and drought. The purpose of this exp...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.934964/full |
| _version_ | 1818499103962693632 |
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| author | Yanyan Han Wenrui Zhang Tingying Xu Ming Tang Ming Tang |
| author_facet | Yanyan Han Wenrui Zhang Tingying Xu Ming Tang Ming Tang |
| author_sort | Yanyan Han |
| collection | DOAJ |
| description | The application of arbuscular mycorrhizal fungi (AM fungi) and phosphorus (P) can improve plant growth under drought stress by upregulating the antioxidant system and osmotic accumulation. The 14-3-3 protein can respond to different abiotic stresses such as low P and drought. The purpose of this experiment was to study the effects of AM fungi (Rhizophagus intraradices) inoculation on reactive oxygen species (ROS) homeostasis, P metabolism, and 14-3-3 gene expression of Populus cathayana at different P levels and drought stress (WW: well-watered and WD: water deficit). Under WD conditions, AM fungi inoculation significantly increased the P content in leaves and roots, but the benefit in roots is limited by the level of P addition, and the roots may have more alkaline phosphatase and phytase under P stress, and these activities in the rhizosphere soil inoculated with AM fungi were stronger. Under WD conditions, the activities of catalase (leaf and root) and peroxidase (root) inoculated with AM fungi were significantly higher than those without inoculation and decreased with P addition. 14-3-3 genes, PcGRF10 and PcGRF11, have a positive correlation with the antioxidant system, osmotic regulation, and P metabolism, which may be more significant after inoculation with AM fungi. Our results provide new insights into the mechanism of ROS homeostasis and P metabolism in mycorrhizal plants under drought stress. |
| first_indexed | 2024-12-10T20:24:41Z |
| format | Article |
| id | doaj.art-4444fae8f6ba4abc86e564109f8946a8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T20:24:41Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4444fae8f6ba4abc86e564109f8946a82022-12-22T01:34:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.934964934964Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayanaYanyan Han0Wenrui Zhang1Tingying Xu2Ming Tang3Ming Tang4College of Forestry, Northwest A&F University, Xianyang, ChinaCollege of Forestry, Northwest A&F University, Xianyang, ChinaBoone Pickens School of Geology, Oklahoma State University, Stillwater, OK, United StatesCollege of Forestry, Northwest A&F University, Xianyang, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaThe application of arbuscular mycorrhizal fungi (AM fungi) and phosphorus (P) can improve plant growth under drought stress by upregulating the antioxidant system and osmotic accumulation. The 14-3-3 protein can respond to different abiotic stresses such as low P and drought. The purpose of this experiment was to study the effects of AM fungi (Rhizophagus intraradices) inoculation on reactive oxygen species (ROS) homeostasis, P metabolism, and 14-3-3 gene expression of Populus cathayana at different P levels and drought stress (WW: well-watered and WD: water deficit). Under WD conditions, AM fungi inoculation significantly increased the P content in leaves and roots, but the benefit in roots is limited by the level of P addition, and the roots may have more alkaline phosphatase and phytase under P stress, and these activities in the rhizosphere soil inoculated with AM fungi were stronger. Under WD conditions, the activities of catalase (leaf and root) and peroxidase (root) inoculated with AM fungi were significantly higher than those without inoculation and decreased with P addition. 14-3-3 genes, PcGRF10 and PcGRF11, have a positive correlation with the antioxidant system, osmotic regulation, and P metabolism, which may be more significant after inoculation with AM fungi. Our results provide new insights into the mechanism of ROS homeostasis and P metabolism in mycorrhizal plants under drought stress.https://www.frontiersin.org/articles/10.3389/fmicb.2022.934964/fullarbuscular mycorrhizal fungiphosphorus metabolismdrought stressreactive oxygen species homeostasis14-3-3 proteins |
| spellingShingle | Yanyan Han Wenrui Zhang Tingying Xu Ming Tang Ming Tang Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana Frontiers in Microbiology arbuscular mycorrhizal fungi phosphorus metabolism drought stress reactive oxygen species homeostasis 14-3-3 proteins |
| title | Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana |
| title_full | Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana |
| title_fullStr | Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana |
| title_full_unstemmed | Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana |
| title_short | Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana |
| title_sort | effect of arbuscular mycorrhizal fungi and phosphorus on drought induced oxidative stress and 14 3 3 proteins gene expression of populus cathayana |
| topic | arbuscular mycorrhizal fungi phosphorus metabolism drought stress reactive oxygen species homeostasis 14-3-3 proteins |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.934964/full |
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