Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni
Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-05-01
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| Series: | Chemosensors |
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| Online Access: | http://www.mdpi.com/2227-9040/5/2/16 |
| _version_ | 1819128618822926336 |
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| author | Noor Azlina Masdor Zeynep Altintas Ibtisam E. Tothill |
| author_facet | Noor Azlina Masdor Zeynep Altintas Ibtisam E. Tothill |
| author_sort | Noor Azlina Masdor |
| collection | DOAJ |
| description | Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 106 CFU·mL−1. The sensitivity of the assay was further enhanced to 4 × 104 CFU·mL−1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (106–107 CFU·mL−1). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni. |
| first_indexed | 2024-12-22T08:30:41Z |
| format | Article |
| id | doaj.art-444672a9b756438eba9b03470e329aea |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-12-22T08:30:41Z |
| publishDate | 2017-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-444672a9b756438eba9b03470e329aea2022-12-21T18:32:30ZengMDPI AGChemosensors2227-90402017-05-01521610.3390/chemosensors5020016chemosensors5020016Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuniNoor Azlina Masdor0Zeynep Altintas1Ibtisam E. Tothill2Surface Engineering and Nanotechnology Institute, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UKSurface Engineering and Nanotechnology Institute, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UKSurface Engineering and Nanotechnology Institute, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UKCampylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 106 CFU·mL−1. The sensitivity of the assay was further enhanced to 4 × 104 CFU·mL−1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (106–107 CFU·mL−1). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni.http://www.mdpi.com/2227-9040/5/2/16Campylobacter jejunicampylobacteriosisSurface Plasmon Resonance (SPR)immunosensorsandwich assay |
| spellingShingle | Noor Azlina Masdor Zeynep Altintas Ibtisam E. Tothill Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni Chemosensors Campylobacter jejuni campylobacteriosis Surface Plasmon Resonance (SPR) immunosensor sandwich assay |
| title | Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni |
| title_full | Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni |
| title_fullStr | Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni |
| title_full_unstemmed | Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni |
| title_short | Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni |
| title_sort | surface plasmon resonance immunosensor for the detection of campylobacter jejuni |
| topic | Campylobacter jejuni campylobacteriosis Surface Plasmon Resonance (SPR) immunosensor sandwich assay |
| url | http://www.mdpi.com/2227-9040/5/2/16 |
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