Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2 [version 1; referees: 2 approved]
Eukaryotic protein translation is divided into three mains stages: initiation, elongation and termination. Regulation of this process occurs at the initiation and elongation step. eEF2 kinase phosphorylates eEF2 factor, blocking its ribosome interaction and thus translation elongation. This kinase a...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2015-09-01
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| Series: | F1000Research |
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| Online Access: | https://f1000research.com/articles/4-902/v1 |
| _version_ | 1819181892729044992 |
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| author | Viviane Alves |
| author_facet | Viviane Alves |
| author_sort | Viviane Alves |
| collection | DOAJ |
| description | Eukaryotic protein translation is divided into three mains stages: initiation, elongation and termination. Regulation of this process occurs at the initiation and elongation step. eEF2 kinase phosphorylates eEF2 factor, blocking its ribosome interaction and thus translation elongation. This kinase activity can be detected by measuring eEF2 phosphorylation status. Here I show that vertebrate-specific antibody against phospho-eEF2 has excellent reactivity against C. elegans orthologue protein phospho-EEF-2. |
| first_indexed | 2024-12-22T22:37:27Z |
| format | Article |
| id | doaj.art-44481813ce364e8e9cec81226bbfbe7e |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-22T22:37:27Z |
| publishDate | 2015-09-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-44481813ce364e8e9cec81226bbfbe7e2022-12-21T18:10:16ZengF1000 Research LtdF1000Research2046-14022015-09-01410.12688/f1000research.7127.17676Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2 [version 1; referees: 2 approved]Viviane Alves0Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, BrazilEukaryotic protein translation is divided into three mains stages: initiation, elongation and termination. Regulation of this process occurs at the initiation and elongation step. eEF2 kinase phosphorylates eEF2 factor, blocking its ribosome interaction and thus translation elongation. This kinase activity can be detected by measuring eEF2 phosphorylation status. Here I show that vertebrate-specific antibody against phospho-eEF2 has excellent reactivity against C. elegans orthologue protein phospho-EEF-2.https://f1000research.com/articles/4-902/v1Cell SignalingProtein Chemistry & Proteomics |
| spellingShingle | Viviane Alves Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2 [version 1; referees: 2 approved] F1000Research Cell Signaling Protein Chemistry & Proteomics |
| title | Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2 [version 1; referees: 2 approved] |
| title_full | Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2 [version 1; referees: 2 approved] |
| title_fullStr | Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2 [version 1; referees: 2 approved] |
| title_full_unstemmed | Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2 [version 1; referees: 2 approved] |
| title_short | Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans orthologue phospho-EEF-2 [version 1; referees: 2 approved] |
| title_sort | reactivity of vertebrate directed phospho eef2 antibody against the caenorhabditis elegans orthologue phospho eef 2 version 1 referees 2 approved |
| topic | Cell Signaling Protein Chemistry & Proteomics |
| url | https://f1000research.com/articles/4-902/v1 |
| work_keys_str_mv | AT vivianealves reactivityofvertebratedirectedphosphoeef2antibodyagainstthecaenorhabditiselegansorthologuephosphoeef2version1referees2approved |