Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphana
Many anthozoans, e.g., sea anemones and corals, have remarkable capacities for asexual reproduction and regeneration, creating complete individual polyps from pieces of parental polyp tissue. Notably, polyps produced asexually (or regenerated) from somatic tissues that originally contain no gonads a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1278022/full |
| _version_ | 1827762637697449984 |
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| author | Pei-Jung Shao Yi-Ling Chiu Pin-Hsuan Tsai Shinya Shikina Shinya Shikina |
| author_facet | Pei-Jung Shao Yi-Ling Chiu Pin-Hsuan Tsai Shinya Shikina Shinya Shikina |
| author_sort | Pei-Jung Shao |
| collection | DOAJ |
| description | Many anthozoans, e.g., sea anemones and corals, have remarkable capacities for asexual reproduction and regeneration, creating complete individual polyps from pieces of parental polyp tissue. Notably, polyps produced asexually (or regenerated) from somatic tissues that originally contain no gonads are capable of sexual reproduction. However, how somatically derived polyps can reproduce remains largely unexplored. Here, using a sea anemone, Exaiptasia diaphana, we show that potential germline progenitor cells are present in the extra-gonadal tissues, and that they are allocated to newly produced individuals during asexual reproduction/regeneration. Histological analysis of E. diaphana demonstrated that polyps asexually produced from pedal lacerates can create mature gametes. Amputation experiments showed that polyps regenerated from the head, which originally lacked gonadal tissue, formed gonads and initiated gametogenesis. Immunohistochemical analysis with a germline marker, vasa, demonstrated the presence of vasa-positive cells in various polyp tissues. These findings suggest that vasa-positive cells in extra-gonadal tissues may be a reservoir of germline progenitor cells to ensure sexual reproductive capacity in individuals produced asexually or regenerated from somatic tissues. |
| first_indexed | 2024-03-11T10:33:26Z |
| format | Article |
| id | doaj.art-4449f97a1a914f49971de146c286edf1 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-11T10:33:26Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-4449f97a1a914f49971de146c286edf12023-11-14T12:14:37ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-11-011010.3389/fmars.2023.12780221278022Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphanaPei-Jung Shao0Yi-Ling Chiu1Pin-Hsuan Tsai2Shinya Shikina3Shinya Shikina4Institute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung, TaiwanInstitute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung, TaiwanInstitute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung, TaiwanInstitute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung, TaiwanCenter of Excellence for the Oceans, National Taiwan Ocean University, Keelung, TaiwanMany anthozoans, e.g., sea anemones and corals, have remarkable capacities for asexual reproduction and regeneration, creating complete individual polyps from pieces of parental polyp tissue. Notably, polyps produced asexually (or regenerated) from somatic tissues that originally contain no gonads are capable of sexual reproduction. However, how somatically derived polyps can reproduce remains largely unexplored. Here, using a sea anemone, Exaiptasia diaphana, we show that potential germline progenitor cells are present in the extra-gonadal tissues, and that they are allocated to newly produced individuals during asexual reproduction/regeneration. Histological analysis of E. diaphana demonstrated that polyps asexually produced from pedal lacerates can create mature gametes. Amputation experiments showed that polyps regenerated from the head, which originally lacked gonadal tissue, formed gonads and initiated gametogenesis. Immunohistochemical analysis with a germline marker, vasa, demonstrated the presence of vasa-positive cells in various polyp tissues. These findings suggest that vasa-positive cells in extra-gonadal tissues may be a reservoir of germline progenitor cells to ensure sexual reproductive capacity in individuals produced asexually or regenerated from somatic tissues.https://www.frontiersin.org/articles/10.3389/fmars.2023.1278022/fullsea anemoneExaiptasia diaphanaVasapedal lacerationreproduction |
| spellingShingle | Pei-Jung Shao Yi-Ling Chiu Pin-Hsuan Tsai Shinya Shikina Shinya Shikina Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphana Frontiers in Marine Science sea anemone Exaiptasia diaphana Vasa pedal laceration reproduction |
| title | Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphana |
| title_full | Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphana |
| title_fullStr | Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphana |
| title_full_unstemmed | Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphana |
| title_short | Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphana |
| title_sort | possible germline progenitor cells in extra gonadal tissues of the sea anemone exaiptasia diaphana |
| topic | sea anemone Exaiptasia diaphana Vasa pedal laceration reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1278022/full |
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