Morphological evaluation of the quality of decellularized esophagus in rat
The main aim of our investigation was analysis and optimization of the existing protocols of esophagus decellularization in rats, identification of parameters affecting the quality of decellularization and intactness of the native properties of esophageal extracellular matrix (ECM). Materials and me...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2017-06-01
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| Series: | Вестник трансплантологии и искусственных органов |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/757 |
| _version_ | 1826546350521581568 |
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| author | R. Z. Nakokhov E. A. Gubareva E. V. Kuevda A. S. Sotnichenko I. S. Gumenyuk A. Kh. Kade G. M. Mogilnaya D. P. Puzanov |
| author_facet | R. Z. Nakokhov E. A. Gubareva E. V. Kuevda A. S. Sotnichenko I. S. Gumenyuk A. Kh. Kade G. M. Mogilnaya D. P. Puzanov |
| author_sort | R. Z. Nakokhov |
| collection | DOAJ |
| description | The main aim of our investigation was analysis and optimization of the existing protocols of esophagus decellularization in rats, identification of parameters affecting the quality of decellularization and intactness of the native properties of esophageal extracellular matrix (ECM). Materials and methods. We developed a modified decellularization protocol based on detergent-enzymatic method using sodium deoxycholate and DNAse. Results. Morphological evaluation of the obtained decellularized matrices demonstrated the absence of cells and cell elements while mechanical properties of ECM were preserved. Conclusion. The developed protocol of esophagus decellularization is a potential basis for obtaining of tissue-engineered esophagus scaffold constructions. |
| first_indexed | 2024-04-10T00:52:48Z |
| format | Article |
| id | doaj.art-444bb04ada3b404ca0e5ed401d129c0a |
| institution | Directory Open Access Journal |
| issn | 1995-1191 |
| language | Russian |
| last_indexed | 2025-03-14T05:32:22Z |
| publishDate | 2017-06-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj.art-444bb04ada3b404ca0e5ed401d129c0a2025-03-05T14:11:49ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912017-06-011929810310.15825/1995-1191-2017-2-98-103628Morphological evaluation of the quality of decellularized esophagus in ratR. Z. Nakokhov0E. A. Gubareva1E. V. Kuevda2A. S. Sotnichenko3I. S. Gumenyuk4A. Kh. Kade5G. M. Mogilnaya6D. P. Puzanov7Laboratory of fundamental research in the field of regenerative medicine, Kuban State Medical UniversityLaboratory of fundamental research in the field of regenerative medicine, Kuban State Medical UniversityLaboratory of fundamental research in the field of regenerative medicine, Kuban State Medical UniversityLaboratory of fundamental research in the field of regenerative medicine, Kuban State Medical UniversityLaboratory of fundamental research in the field of regenerative medicine, Kuban State Medical UniversityDepartment of General and Clinical Pathophysiology, Kuban State Medical UniversityDepartment of Histology and Embryology, Kuban State Medical UniversityLaboratory of fundamental research in the field of regenerative medicine, Kuban State Medical UniversityThe main aim of our investigation was analysis and optimization of the existing protocols of esophagus decellularization in rats, identification of parameters affecting the quality of decellularization and intactness of the native properties of esophageal extracellular matrix (ECM). Materials and methods. We developed a modified decellularization protocol based on detergent-enzymatic method using sodium deoxycholate and DNAse. Results. Morphological evaluation of the obtained decellularized matrices demonstrated the absence of cells and cell elements while mechanical properties of ECM were preserved. Conclusion. The developed protocol of esophagus decellularization is a potential basis for obtaining of tissue-engineered esophagus scaffold constructions.https://journal.transpl.ru/vtio/article/view/757regenerative medicineesophagusdecellularizationtissue-engineered constructionextracellular matrixscaffold |
| spellingShingle | R. Z. Nakokhov E. A. Gubareva E. V. Kuevda A. S. Sotnichenko I. S. Gumenyuk A. Kh. Kade G. M. Mogilnaya D. P. Puzanov Morphological evaluation of the quality of decellularized esophagus in rat Вестник трансплантологии и искусственных органов regenerative medicine esophagus decellularization tissue-engineered construction extracellular matrix scaffold |
| title | Morphological evaluation of the quality of decellularized esophagus in rat |
| title_full | Morphological evaluation of the quality of decellularized esophagus in rat |
| title_fullStr | Morphological evaluation of the quality of decellularized esophagus in rat |
| title_full_unstemmed | Morphological evaluation of the quality of decellularized esophagus in rat |
| title_short | Morphological evaluation of the quality of decellularized esophagus in rat |
| title_sort | morphological evaluation of the quality of decellularized esophagus in rat |
| topic | regenerative medicine esophagus decellularization tissue-engineered construction extracellular matrix scaffold |
| url | https://journal.transpl.ru/vtio/article/view/757 |
| work_keys_str_mv | AT rznakokhov morphologicalevaluationofthequalityofdecellularizedesophagusinrat AT eagubareva morphologicalevaluationofthequalityofdecellularizedesophagusinrat AT evkuevda morphologicalevaluationofthequalityofdecellularizedesophagusinrat AT assotnichenko morphologicalevaluationofthequalityofdecellularizedesophagusinrat AT isgumenyuk morphologicalevaluationofthequalityofdecellularizedesophagusinrat AT akhkade morphologicalevaluationofthequalityofdecellularizedesophagusinrat AT gmmogilnaya morphologicalevaluationofthequalityofdecellularizedesophagusinrat AT dppuzanov morphologicalevaluationofthequalityofdecellularizedesophagusinrat |