An analysis of intestinal morphology and incretin-producing cells using tissue optical clearing and 3-D imaging
Abstract Tissue optical clearing permits detailed evaluation of organ three-dimensional (3-D) structure as well as that of individual cells by tissue staining and autofluorescence. In this study, we evaluated intestinal morphology, intestinal epithelial cells (IECs), and enteroendocrine cells, such...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-22511-7 |
| _version_ | 1797986016393428992 |
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| author | Tomonobu Hatoko Norio Harada Shinsuke Tokumoto Shunsuke Yamane Eri Ikeguchi-Ogura Tomoko Kato Takuma Yasuda Hisato Tatsuoka Satoko Shimazu-Kuwahara Daisuke Yabe Yoshitaka Hayashi Nobuya Inagaki |
| author_facet | Tomonobu Hatoko Norio Harada Shinsuke Tokumoto Shunsuke Yamane Eri Ikeguchi-Ogura Tomoko Kato Takuma Yasuda Hisato Tatsuoka Satoko Shimazu-Kuwahara Daisuke Yabe Yoshitaka Hayashi Nobuya Inagaki |
| author_sort | Tomonobu Hatoko |
| collection | DOAJ |
| description | Abstract Tissue optical clearing permits detailed evaluation of organ three-dimensional (3-D) structure as well as that of individual cells by tissue staining and autofluorescence. In this study, we evaluated intestinal morphology, intestinal epithelial cells (IECs), and enteroendocrine cells, such as incretin-producing cells, in reporter mice by intestinal 3-D imaging. 3-D intestinal imaging of reporter mice using optical tissue clearing enabled us to evaluate both detailed intestinal morphologies and cell numbers, villus length and crypt depth in the same samples. In disease mouse model of lipopolysaccharide (LPS)-injected mice, the results of 3-D imaging using tissue optical clearing in this study was consistent with those of 2-D imaging in previous reports and could added the new data of intestinal morphology. In analysis of incretin-producing cells of reporter mice, we could elucidate the number, the percentage, and the localization of incretin-producing cells in intestine and the difference of those between L cells and K cells. Thus, we established a novel method of intestinal analysis using tissue optical clearing and 3-D imaging. 3-D evaluation of intestine enabled us to clarify not only detailed intestinal morphology but also the precise number and localization of IECs and incretin-producing cells in the same samples. |
| first_indexed | 2024-04-11T07:27:21Z |
| format | Article |
| id | doaj.art-444c2be1c89a4867a8b89ba4bb7497c6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T07:27:21Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-444c2be1c89a4867a8b89ba4bb7497c62022-12-22T04:37:02ZengNature PortfolioScientific Reports2045-23222022-10-0112111210.1038/s41598-022-22511-7An analysis of intestinal morphology and incretin-producing cells using tissue optical clearing and 3-D imagingTomonobu Hatoko0Norio Harada1Shinsuke Tokumoto2Shunsuke Yamane3Eri Ikeguchi-Ogura4Tomoko Kato5Takuma Yasuda6Hisato Tatsuoka7Satoko Shimazu-Kuwahara8Daisuke Yabe9Yoshitaka Hayashi10Nobuya Inagaki11Department of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineDepartment of Diabetes and Endocrinology, Graduate School of Medicine, Gifu UniversityDivision of Stress Adaptation and Protection, Department of Endocrinology, Research Institute of Environmental Medicine, Nagoya UniversityDepartment of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of MedicineAbstract Tissue optical clearing permits detailed evaluation of organ three-dimensional (3-D) structure as well as that of individual cells by tissue staining and autofluorescence. In this study, we evaluated intestinal morphology, intestinal epithelial cells (IECs), and enteroendocrine cells, such as incretin-producing cells, in reporter mice by intestinal 3-D imaging. 3-D intestinal imaging of reporter mice using optical tissue clearing enabled us to evaluate both detailed intestinal morphologies and cell numbers, villus length and crypt depth in the same samples. In disease mouse model of lipopolysaccharide (LPS)-injected mice, the results of 3-D imaging using tissue optical clearing in this study was consistent with those of 2-D imaging in previous reports and could added the new data of intestinal morphology. In analysis of incretin-producing cells of reporter mice, we could elucidate the number, the percentage, and the localization of incretin-producing cells in intestine and the difference of those between L cells and K cells. Thus, we established a novel method of intestinal analysis using tissue optical clearing and 3-D imaging. 3-D evaluation of intestine enabled us to clarify not only detailed intestinal morphology but also the precise number and localization of IECs and incretin-producing cells in the same samples.https://doi.org/10.1038/s41598-022-22511-7 |
| spellingShingle | Tomonobu Hatoko Norio Harada Shinsuke Tokumoto Shunsuke Yamane Eri Ikeguchi-Ogura Tomoko Kato Takuma Yasuda Hisato Tatsuoka Satoko Shimazu-Kuwahara Daisuke Yabe Yoshitaka Hayashi Nobuya Inagaki An analysis of intestinal morphology and incretin-producing cells using tissue optical clearing and 3-D imaging Scientific Reports |
| title | An analysis of intestinal morphology and incretin-producing cells using tissue optical clearing and 3-D imaging |
| title_full | An analysis of intestinal morphology and incretin-producing cells using tissue optical clearing and 3-D imaging |
| title_fullStr | An analysis of intestinal morphology and incretin-producing cells using tissue optical clearing and 3-D imaging |
| title_full_unstemmed | An analysis of intestinal morphology and incretin-producing cells using tissue optical clearing and 3-D imaging |
| title_short | An analysis of intestinal morphology and incretin-producing cells using tissue optical clearing and 3-D imaging |
| title_sort | analysis of intestinal morphology and incretin producing cells using tissue optical clearing and 3 d imaging |
| url | https://doi.org/10.1038/s41598-022-22511-7 |
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