Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis
Background: The humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. Nuclear factor-kappa B dimers are encoded by nuclear factor-kappa B genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis f...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2018-02-01
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| Series: | Balkan Medical Journal |
| Subjects: | |
| Online Access: | http://balkanmedicaljournal.org/text.php?lang=en&id=1779 |
| _version_ | 1797923242398187520 |
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| author | Leyla Acar Nazan Atalan E. Hande Karagedik Arzu Ergen |
| author_facet | Leyla Acar Nazan Atalan E. Hande Karagedik Arzu Ergen |
| author_sort | Leyla Acar |
| collection | DOAJ |
| description | Background: The humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. Nuclear factor-kappa B dimers are encoded by nuclear factor-kappa B genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis factor-alpha.
Aims: To investigate the possible effect of polymorphisms on tumour necrosis factor-alpha serum levels with clinical and prognostic parameters of sepsis by determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A) gene polymorphisms and tumour necrosis factor-alpha serum levels.
Study Design: Case-control study.
Methods: Seventy-two patients with sepsis and 104 healthy controls were included in the study. In order to determine the polymorphisms of nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A), polymerase chain reaction–restriction fragment length polymorphism analysis was performed and serum tumour necrosis factor-alpha levels were determined using an enzyme-linked immunosorbent assay.
Results: We observed no significant differences in tumour necrosis factor-alpha serum levels between the study groups. In the patient group, an increase in the tumour necrosis factor-alpha serum levels in patients carrying the tumour necrosis factor-alpha (-308 G/A) A allele compared to those without the A allele was found to be statistically significant. Additionally, an increase in the tumour necrosis factor-alpha serum levels in patients carrying tumour necrosis factor-alpha (-308 G/A) AA genotype compared with patients carrying the AG or GG genotypes was statistically significant. No significant differences were found in these 2 polymorphisms between the patient and control groups (p>0.05).
Conclusion: Our results showed the AA genotype and the A allele of the tumour necrosis factor-alpha (-308 G/A) polymorphism may be used as a predictor of elevated tumour necrosis factor-alpha levels in patients with sepsis |
| first_indexed | 2024-04-10T14:43:30Z |
| format | Article |
| id | doaj.art-444c672afe324f0ea1d7f6fb5e9c05dd |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T14:43:30Z |
| publishDate | 2018-02-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-444c672afe324f0ea1d7f6fb5e9c05dd2023-02-15T16:08:01ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312018-02-01351303510.4274/balkanmedj.2017.0246Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in SepsisLeyla Acar0Nazan Atalan1E. Hande Karagedik2Arzu Ergen3Department of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, TurkeyClinic of Anesthesia and Reanimation, Siyami Ersek Thoracic Cardiovascular Surgery Training and Research Hospital, İstanbul, TurkeyDepartment of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, TurkeyDepartment of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, TurkeyBackground: The humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. Nuclear factor-kappa B dimers are encoded by nuclear factor-kappa B genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis factor-alpha. Aims: To investigate the possible effect of polymorphisms on tumour necrosis factor-alpha serum levels with clinical and prognostic parameters of sepsis by determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A) gene polymorphisms and tumour necrosis factor-alpha serum levels. Study Design: Case-control study. Methods: Seventy-two patients with sepsis and 104 healthy controls were included in the study. In order to determine the polymorphisms of nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A), polymerase chain reaction–restriction fragment length polymorphism analysis was performed and serum tumour necrosis factor-alpha levels were determined using an enzyme-linked immunosorbent assay. Results: We observed no significant differences in tumour necrosis factor-alpha serum levels between the study groups. In the patient group, an increase in the tumour necrosis factor-alpha serum levels in patients carrying the tumour necrosis factor-alpha (-308 G/A) A allele compared to those without the A allele was found to be statistically significant. Additionally, an increase in the tumour necrosis factor-alpha serum levels in patients carrying tumour necrosis factor-alpha (-308 G/A) AA genotype compared with patients carrying the AG or GG genotypes was statistically significant. No significant differences were found in these 2 polymorphisms between the patient and control groups (p>0.05). Conclusion: Our results showed the AA genotype and the A allele of the tumour necrosis factor-alpha (-308 G/A) polymorphism may be used as a predictor of elevated tumour necrosis factor-alpha levels in patients with sepsishttp://balkanmedicaljournal.org/text.php?lang=en&id=1779Sepsispolymorphismtumor necrosis factor-alphaNF-kappa B |
| spellingShingle | Leyla Acar Nazan Atalan E. Hande Karagedik Arzu Ergen Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis Balkan Medical Journal Sepsis polymorphism tumor necrosis factor-alpha NF-kappa B |
| title | Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis |
| title_full | Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis |
| title_fullStr | Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis |
| title_full_unstemmed | Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis |
| title_short | Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis |
| title_sort | tumour necrosis factor alpha and nuclear factor kappa b gene variants in sepsis |
| topic | Sepsis polymorphism tumor necrosis factor-alpha NF-kappa B |
| url | http://balkanmedicaljournal.org/text.php?lang=en&id=1779 |
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