Efflux <i>MexAB</i>-Mediated Resistance in <i>P. aeruginosa</i> Isolated from Patients with Healthcare Associated Infections
Today, one of the most important challenges for physicians is the adequate treatment of infections due to multidrug resistant organism (MDR). <i>Pseudomonas aeruginosa</i> is considered an opportunistic organism causing different types of healthcare associated infections (HAIs). We aimed...
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MDPI AG
2020-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/6/471 |
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| author | Rania M. Kishk Mohamed O. Abdalla Abdullah A. Hashish Nader A. Nemr Nihal El Nahhas Saad Alkahtani Mohamed M. Abdel-Daim Safaa M. Kishk |
| author_facet | Rania M. Kishk Mohamed O. Abdalla Abdullah A. Hashish Nader A. Nemr Nihal El Nahhas Saad Alkahtani Mohamed M. Abdel-Daim Safaa M. Kishk |
| author_sort | Rania M. Kishk |
| collection | DOAJ |
| description | Today, one of the most important challenges for physicians is the adequate treatment of infections due to multidrug resistant organism (MDR). <i>Pseudomonas aeruginosa</i> is considered an opportunistic organism causing different types of healthcare associated infections (HAIs). We aimed to investigate the MDR and pandrug resistance (PDR) rate in <i>P. aeruginosa</i> in our region and detect efflux-pump <i>mexAB</i> genes and the proposed binding interactions of five different categories of antimicrobial agents with the <i>mexB</i> pump. A total of 180 non-duplicated <i>P. aeruginosa strains</i> were isolated from patients with HAIs in the Suez Canal University Hospital. Phenotypically, minimum inhibitory concentration (MIC) was done for all MDR and PDR strains before and after addition of efflux pump inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP). Molecular detection of <i>mexA</i> and <i>mexB</i> genes was done by using polymerase chain reaction (PCR). Most of the isolated strains (126 strains) were MDR (70%); only 10 samples (5.5%) were PDR. <i>MexA</i> and <i>mexB</i> genes were detected in 88.2% (120 strains) and 70.5% (96 strains) of stains, respectively. All PDR strains (10 stains) carried both <i>mexA</i> and <i>mexB</i> genes. Efflux <i>mexAB</i> genes were detected in all MDR and PDR strains (136 strains). Molecular modeling studies were performed to investigate the modes of intermolecular binding interactions between the antimicrobial agents and <i>mexB</i> key amino acids that resulted in MDR and PDR. The current study reported high prevalence of MDR and PDR <i>P. aeruginosa</i> in patients with HAIs in the Suez Canal University Hospitals. |
| first_indexed | 2024-03-10T19:10:00Z |
| format | Article |
| id | doaj.art-4454d9317a5342a6b1e126bdcbdaa740 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T19:10:00Z |
| publishDate | 2020-06-01 |
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| series | Pathogens |
| spelling | doaj.art-4454d9317a5342a6b1e126bdcbdaa7402023-11-20T03:52:52ZengMDPI AGPathogens2076-08172020-06-019647110.3390/pathogens9060471Efflux <i>MexAB</i>-Mediated Resistance in <i>P. aeruginosa</i> Isolated from Patients with Healthcare Associated InfectionsRania M. Kishk0Mohamed O. Abdalla1Abdullah A. Hashish2Nader A. Nemr3Nihal El Nahhas4Saad Alkahtani5Mohamed M. Abdel-Daim6Safaa M. Kishk7Department of Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptEndemic and Infectious Diseases, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Botany, Faculty of Science, Alexandria University, Moharram baik, Alexandria 21515, EgyptDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Medicinal Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptToday, one of the most important challenges for physicians is the adequate treatment of infections due to multidrug resistant organism (MDR). <i>Pseudomonas aeruginosa</i> is considered an opportunistic organism causing different types of healthcare associated infections (HAIs). We aimed to investigate the MDR and pandrug resistance (PDR) rate in <i>P. aeruginosa</i> in our region and detect efflux-pump <i>mexAB</i> genes and the proposed binding interactions of five different categories of antimicrobial agents with the <i>mexB</i> pump. A total of 180 non-duplicated <i>P. aeruginosa strains</i> were isolated from patients with HAIs in the Suez Canal University Hospital. Phenotypically, minimum inhibitory concentration (MIC) was done for all MDR and PDR strains before and after addition of efflux pump inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP). Molecular detection of <i>mexA</i> and <i>mexB</i> genes was done by using polymerase chain reaction (PCR). Most of the isolated strains (126 strains) were MDR (70%); only 10 samples (5.5%) were PDR. <i>MexA</i> and <i>mexB</i> genes were detected in 88.2% (120 strains) and 70.5% (96 strains) of stains, respectively. All PDR strains (10 stains) carried both <i>mexA</i> and <i>mexB</i> genes. Efflux <i>mexAB</i> genes were detected in all MDR and PDR strains (136 strains). Molecular modeling studies were performed to investigate the modes of intermolecular binding interactions between the antimicrobial agents and <i>mexB</i> key amino acids that resulted in MDR and PDR. The current study reported high prevalence of MDR and PDR <i>P. aeruginosa</i> in patients with HAIs in the Suez Canal University Hospitals.https://www.mdpi.com/2076-0817/9/6/471efflux pumpMexAB<i>Pseudomonas aeruginosa</i>HAIsEgyptmolecular modeling |
| spellingShingle | Rania M. Kishk Mohamed O. Abdalla Abdullah A. Hashish Nader A. Nemr Nihal El Nahhas Saad Alkahtani Mohamed M. Abdel-Daim Safaa M. Kishk Efflux <i>MexAB</i>-Mediated Resistance in <i>P. aeruginosa</i> Isolated from Patients with Healthcare Associated Infections Pathogens efflux pump MexAB <i>Pseudomonas aeruginosa</i> HAIs Egypt molecular modeling |
| title | Efflux <i>MexAB</i>-Mediated Resistance in <i>P. aeruginosa</i> Isolated from Patients with Healthcare Associated Infections |
| title_full | Efflux <i>MexAB</i>-Mediated Resistance in <i>P. aeruginosa</i> Isolated from Patients with Healthcare Associated Infections |
| title_fullStr | Efflux <i>MexAB</i>-Mediated Resistance in <i>P. aeruginosa</i> Isolated from Patients with Healthcare Associated Infections |
| title_full_unstemmed | Efflux <i>MexAB</i>-Mediated Resistance in <i>P. aeruginosa</i> Isolated from Patients with Healthcare Associated Infections |
| title_short | Efflux <i>MexAB</i>-Mediated Resistance in <i>P. aeruginosa</i> Isolated from Patients with Healthcare Associated Infections |
| title_sort | efflux i mexab i mediated resistance in i p aeruginosa i isolated from patients with healthcare associated infections |
| topic | efflux pump MexAB <i>Pseudomonas aeruginosa</i> HAIs Egypt molecular modeling |
| url | https://www.mdpi.com/2076-0817/9/6/471 |
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