Effectiveness of Toothpastes on SARS-CoV-2 Viral Load in Saliva
ABSTRACT: Introduction: The effect of toothpastes on viruses, such as SARS-CoV-2, is unknown. This study investigated the short-term effect of toothpastes containing antimicrobial properties in patients with novel coronavirus disease 2019 (COVID-19) to determine whether they could reduce the SARS-C...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | International Dental Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0020653922000697 |
| _version_ | 1797988757501116416 |
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| author | Fernanda P Eduardo Luciana Corrêa Fernanda Mansur Carlos Benitez Nelson Hamerschlak João Renato Rebello Pinho Debora Heller Letícia Mello Bezinelli |
| author_facet | Fernanda P Eduardo Luciana Corrêa Fernanda Mansur Carlos Benitez Nelson Hamerschlak João Renato Rebello Pinho Debora Heller Letícia Mello Bezinelli |
| author_sort | Fernanda P Eduardo |
| collection | DOAJ |
| description | ABSTRACT: Introduction: The effect of toothpastes on viruses, such as SARS-CoV-2, is unknown. This study investigated the short-term effect of toothpastes containing antimicrobial properties in patients with novel coronavirus disease 2019 (COVID-19) to determine whether they could reduce the SARS-CoV-2 salivary viral load. Methods: Hospitalised patients with COVID-19 (n = 83) were instructed to perform toothbrushing with 1 of 3 arms: a toothpaste containing 0.96% zinc (zinc oxide, zinc citrate) in a silica base (Test 1), a toothpaste containing 0.454% SnF2 in a silica base (Test 2), and a nonantibacterial toothpaste (control). Saliva was collected before intervention (T0), immediately after intervention (T1), and 30 (T2) and 60 minutes (T3) after intervention. The SARS-CoV-2 salivary viral load was measured using quantitative real-time polymerase chain reaction (qRT-PCR) assays. For Test 1 and Test 2 toothpastes, the fold reductions were normalised to baseline and to the control toothpaste at each time point after brushing. A fold change of ≥2 is considered clinically effective. Results: Brushing with the Test 1 toothpaste reduced the SARS-CoV-2 salivary viral load by 4.06-fold at T1, by 2.36-fold at T2, and by 1.42-fold at T3. Similarly, brushing with a Test 2 toothpaste reduced the SARS-CoV-2 salivary viral load by 2.33-fold at T1, by 2.38-fold at T2, and by 0.77-fold at T3. Conclusions: Immediately after brushing, the use of antimicrobial toothpastes reduced the salivary viral load of patients with COVID-19. The trial was registered on https://clinicaltrials.gov/ (NCT04537962). |
| first_indexed | 2024-04-11T08:07:58Z |
| format | Article |
| id | doaj.art-44552f708eeb4b848bcc077759ec313f |
| institution | Directory Open Access Journal |
| issn | 0020-6539 |
| language | English |
| last_indexed | 2024-04-11T08:07:58Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Dental Journal |
| spelling | doaj.art-44552f708eeb4b848bcc077759ec313f2022-12-22T04:35:27ZengElsevierInternational Dental Journal0020-65392022-12-01726825831Effectiveness of Toothpastes on SARS-CoV-2 Viral Load in SalivaFernanda P Eduardo0Luciana Corrêa1Fernanda Mansur2Carlos Benitez3Nelson Hamerschlak4João Renato Rebello Pinho5Debora Heller6Letícia Mello Bezinelli7Hospital Israelita Albert Einstein, São Paulo, Brazil; Corresponding author. Hospital Israelita Albert Einstein, Av. Albert Einstein, 627/520 São Paulo, São Paulo, Brazil, CEP 05651-901.School of Dentistry, University of São Paulo, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilLatin American Oral Health Association, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, Brazil; Postgraduate Program in Dentistry, Universidade Cruzeiro do Sul, São Paulo, Brazil; Department of Periodontology, School of Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, USAHospital Israelita Albert Einstein, São Paulo, BrazilABSTRACT: Introduction: The effect of toothpastes on viruses, such as SARS-CoV-2, is unknown. This study investigated the short-term effect of toothpastes containing antimicrobial properties in patients with novel coronavirus disease 2019 (COVID-19) to determine whether they could reduce the SARS-CoV-2 salivary viral load. Methods: Hospitalised patients with COVID-19 (n = 83) were instructed to perform toothbrushing with 1 of 3 arms: a toothpaste containing 0.96% zinc (zinc oxide, zinc citrate) in a silica base (Test 1), a toothpaste containing 0.454% SnF2 in a silica base (Test 2), and a nonantibacterial toothpaste (control). Saliva was collected before intervention (T0), immediately after intervention (T1), and 30 (T2) and 60 minutes (T3) after intervention. The SARS-CoV-2 salivary viral load was measured using quantitative real-time polymerase chain reaction (qRT-PCR) assays. For Test 1 and Test 2 toothpastes, the fold reductions were normalised to baseline and to the control toothpaste at each time point after brushing. A fold change of ≥2 is considered clinically effective. Results: Brushing with the Test 1 toothpaste reduced the SARS-CoV-2 salivary viral load by 4.06-fold at T1, by 2.36-fold at T2, and by 1.42-fold at T3. Similarly, brushing with a Test 2 toothpaste reduced the SARS-CoV-2 salivary viral load by 2.33-fold at T1, by 2.38-fold at T2, and by 0.77-fold at T3. Conclusions: Immediately after brushing, the use of antimicrobial toothpastes reduced the salivary viral load of patients with COVID-19. The trial was registered on https://clinicaltrials.gov/ (NCT04537962).http://www.sciencedirect.com/science/article/pii/S0020653922000697SARS-CoV-2COVID-19ToothpastesVirusesSaliva |
| spellingShingle | Fernanda P Eduardo Luciana Corrêa Fernanda Mansur Carlos Benitez Nelson Hamerschlak João Renato Rebello Pinho Debora Heller Letícia Mello Bezinelli Effectiveness of Toothpastes on SARS-CoV-2 Viral Load in Saliva International Dental Journal SARS-CoV-2 COVID-19 Toothpastes Viruses Saliva |
| title | Effectiveness of Toothpastes on SARS-CoV-2 Viral Load in Saliva |
| title_full | Effectiveness of Toothpastes on SARS-CoV-2 Viral Load in Saliva |
| title_fullStr | Effectiveness of Toothpastes on SARS-CoV-2 Viral Load in Saliva |
| title_full_unstemmed | Effectiveness of Toothpastes on SARS-CoV-2 Viral Load in Saliva |
| title_short | Effectiveness of Toothpastes on SARS-CoV-2 Viral Load in Saliva |
| title_sort | effectiveness of toothpastes on sars cov 2 viral load in saliva |
| topic | SARS-CoV-2 COVID-19 Toothpastes Viruses Saliva |
| url | http://www.sciencedirect.com/science/article/pii/S0020653922000697 |
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