Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS)
Physiological leaf spot (PLS) is a disorder of spring barley (Hordeum vulgare L.), which has become more pronounced in recent years. The initial symptoms are small chlorotic/brown spots on the upper four leaves, which may develop into necrotic lesions with an irregular shape. As PLS occurs on leaves...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Compuscript Ltd
2015-06-01
|
| Series: | Irish Journal of Agricultural and Food Research |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/ijafr.2015.54.issue-1/ijafr-2015-0005/ijafr-2015-0005.xml?format=INT |
| _version_ | 1797703845076271104 |
|---|---|
| author | Moran Mary G. Dix Philip J. Burke James I. |
| author_facet | Moran Mary G. Dix Philip J. Burke James I. |
| author_sort | Moran Mary G. |
| collection | DOAJ |
| description | Physiological leaf spot (PLS) is a disorder of spring barley (Hordeum vulgare L.), which has become more pronounced in recent years. The initial symptoms are small chlorotic/brown spots on the upper four leaves, which may develop into necrotic lesions with an irregular shape. As PLS occurs on leaves that are directly exposed to sunlight, it is thought that high light stress could be a trigger for the condition. This study concentrates on two cultivars, Cooper and Crusader, which display differential sensitivity to PLS. Biochemical measurements and enzyme assays revealed substantial difference in levels of ascorbate, type III peroxidases, and superoxide dismutase between the chosen cultivars during the 2003 growing season. A global gene expression study, using these field samples, was performed by microarray analysis. This supported the biochemical findings and highlighted additional sets of genes differentially expressed between the cultivars. Transcripts of particular interest, which appeared, included calcium signalling genes, cold-responsive genes and those involved in the assembly of Photosystem I. We conclude that susceptibility to PLS is related to levels of expression of genes with a role in countering the effects of oxidative stress. |
| first_indexed | 2024-03-12T05:11:32Z |
| format | Article |
| id | doaj.art-44583c551ec8466193a8aae35c028f02 |
| institution | Directory Open Access Journal |
| issn | 0791-6833 2009-9029 |
| language | English |
| last_indexed | 2024-03-12T05:11:32Z |
| publishDate | 2015-06-01 |
| publisher | Compuscript Ltd |
| record_format | Article |
| series | Irish Journal of Agricultural and Food Research |
| spelling | doaj.art-44583c551ec8466193a8aae35c028f022023-09-03T08:36:56ZengCompuscript LtdIrish Journal of Agricultural and Food Research0791-68332009-90292015-06-01541485810.1515/ijafr-2015-0005ijafr-2015-0005Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS)Moran Mary G.0Dix Philip J.1Burke James I.2Plant Cell Biology Laboratory, Biology Department, National University of Ireland, Maynooth, Co. Kildare, IrelandPlant Cell Biology Laboratory, Biology Department, National University of Ireland, Maynooth, Co. Kildare, IrelandSchool of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, IrelandPhysiological leaf spot (PLS) is a disorder of spring barley (Hordeum vulgare L.), which has become more pronounced in recent years. The initial symptoms are small chlorotic/brown spots on the upper four leaves, which may develop into necrotic lesions with an irregular shape. As PLS occurs on leaves that are directly exposed to sunlight, it is thought that high light stress could be a trigger for the condition. This study concentrates on two cultivars, Cooper and Crusader, which display differential sensitivity to PLS. Biochemical measurements and enzyme assays revealed substantial difference in levels of ascorbate, type III peroxidases, and superoxide dismutase between the chosen cultivars during the 2003 growing season. A global gene expression study, using these field samples, was performed by microarray analysis. This supported the biochemical findings and highlighted additional sets of genes differentially expressed between the cultivars. Transcripts of particular interest, which appeared, included calcium signalling genes, cold-responsive genes and those involved in the assembly of Photosystem I. We conclude that susceptibility to PLS is related to levels of expression of genes with a role in countering the effects of oxidative stress.http://www.degruyter.com/view/j/ijafr.2015.54.issue-1/ijafr-2015-0005/ijafr-2015-0005.xml?format=INTabiotic stressnon-parasitic necrosisphysiological leaf spot |
| spellingShingle | Moran Mary G. Dix Philip J. Burke James I. Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS) Irish Journal of Agricultural and Food Research abiotic stress non-parasitic necrosis physiological leaf spot |
| title | Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS) |
| title_full | Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS) |
| title_fullStr | Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS) |
| title_full_unstemmed | Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS) |
| title_short | Microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot (PLS) |
| title_sort | microarray analysis of spring barley cultivars displaying differing sensitivity to physiological leaf spot pls |
| topic | abiotic stress non-parasitic necrosis physiological leaf spot |
| url | http://www.degruyter.com/view/j/ijafr.2015.54.issue-1/ijafr-2015-0005/ijafr-2015-0005.xml?format=INT |
| work_keys_str_mv | AT moranmaryg microarrayanalysisofspringbarleycultivarsdisplayingdifferingsensitivitytophysiologicalleafspotpls AT dixphilipj microarrayanalysisofspringbarleycultivarsdisplayingdifferingsensitivitytophysiologicalleafspotpls AT burkejamesi microarrayanalysisofspringbarleycultivarsdisplayingdifferingsensitivitytophysiologicalleafspotpls |