miR-4432 Targets FGFBP1 in Human Endothelial Cells
MicroRNAs (miRs) are small non-coding RNAs that modulate the expression of several target genes. Fibroblast growth factor binding protein 1 (FGFBP1) has been associated with endothelial dysfunction at the level of the blood–brain barrier (BBB). However, the underlying mechanisms are mostly unknown a...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/12/3/459 |
| _version_ | 1797613404804874240 |
|---|---|
| author | Roberta Avvisato Pasquale Mone Stanislovas S. Jankauskas Fahimeh Varzideh Urna Kansakar Jessica Gambardella Antonio De Luca Alessandro Matarese Gaetano Santulli |
| author_facet | Roberta Avvisato Pasquale Mone Stanislovas S. Jankauskas Fahimeh Varzideh Urna Kansakar Jessica Gambardella Antonio De Luca Alessandro Matarese Gaetano Santulli |
| author_sort | Roberta Avvisato |
| collection | DOAJ |
| description | MicroRNAs (miRs) are small non-coding RNAs that modulate the expression of several target genes. Fibroblast growth factor binding protein 1 (FGFBP1) has been associated with endothelial dysfunction at the level of the blood–brain barrier (BBB). However, the underlying mechanisms are mostly unknown and there are no studies investigating the relationship between miRs and FGFBP1. Thus, the overarching aim of the present study was to identify and validate which miR can specifically target FGFBP1 in human brain microvascular endothelial cells, which represent the best in vitro model of the BBB. We were able to identify and validate miR-4432 as a fundamental modulator of FGFBP1 and we demonstrated that miR-4432 significantly reduces mitochondrial oxidative stress, a well-established pathophysiological hallmark of hypertension. |
| first_indexed | 2024-03-11T06:54:22Z |
| format | Article |
| id | doaj.art-445a7baf8b504721a32282b2c6b1e7a7 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T06:54:22Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-445a7baf8b504721a32282b2c6b1e7a72023-11-17T09:42:34ZengMDPI AGBiology2079-77372023-03-0112345910.3390/biology12030459miR-4432 Targets FGFBP1 in Human Endothelial CellsRoberta Avvisato0Pasquale Mone1Stanislovas S. Jankauskas2Fahimeh Varzideh3Urna Kansakar4Jessica Gambardella5Antonio De Luca6Alessandro Matarese7Gaetano Santulli8Division of Cardiology, Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USADivision of Cardiology, Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USADivision of Cardiology, Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USADivision of Cardiology, Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USADivision of Cardiology, Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USADivision of Cardiology, Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USADepartment of Mental and Physical Health and Preventive Medicine, University of Campania “<i>Luigi Vanvitelli</i>”, 81100 Caserta, Italy“<i>Antonio Cardarelli</i>” Hospital, 80100 Naples, ItalyDivision of Cardiology, Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USAMicroRNAs (miRs) are small non-coding RNAs that modulate the expression of several target genes. Fibroblast growth factor binding protein 1 (FGFBP1) has been associated with endothelial dysfunction at the level of the blood–brain barrier (BBB). However, the underlying mechanisms are mostly unknown and there are no studies investigating the relationship between miRs and FGFBP1. Thus, the overarching aim of the present study was to identify and validate which miR can specifically target FGFBP1 in human brain microvascular endothelial cells, which represent the best in vitro model of the BBB. We were able to identify and validate miR-4432 as a fundamental modulator of FGFBP1 and we demonstrated that miR-4432 significantly reduces mitochondrial oxidative stress, a well-established pathophysiological hallmark of hypertension.https://www.mdpi.com/2079-7737/12/3/459blood–brain barrierblood pressurecerebrovascular diseaseendothelial dysfunctionhBMECshypertension |
| spellingShingle | Roberta Avvisato Pasquale Mone Stanislovas S. Jankauskas Fahimeh Varzideh Urna Kansakar Jessica Gambardella Antonio De Luca Alessandro Matarese Gaetano Santulli miR-4432 Targets FGFBP1 in Human Endothelial Cells Biology blood–brain barrier blood pressure cerebrovascular disease endothelial dysfunction hBMECs hypertension |
| title | miR-4432 Targets FGFBP1 in Human Endothelial Cells |
| title_full | miR-4432 Targets FGFBP1 in Human Endothelial Cells |
| title_fullStr | miR-4432 Targets FGFBP1 in Human Endothelial Cells |
| title_full_unstemmed | miR-4432 Targets FGFBP1 in Human Endothelial Cells |
| title_short | miR-4432 Targets FGFBP1 in Human Endothelial Cells |
| title_sort | mir 4432 targets fgfbp1 in human endothelial cells |
| topic | blood–brain barrier blood pressure cerebrovascular disease endothelial dysfunction hBMECs hypertension |
| url | https://www.mdpi.com/2079-7737/12/3/459 |
| work_keys_str_mv | AT robertaavvisato mir4432targetsfgfbp1inhumanendothelialcells AT pasqualemone mir4432targetsfgfbp1inhumanendothelialcells AT stanislovassjankauskas mir4432targetsfgfbp1inhumanendothelialcells AT fahimehvarzideh mir4432targetsfgfbp1inhumanendothelialcells AT urnakansakar mir4432targetsfgfbp1inhumanendothelialcells AT jessicagambardella mir4432targetsfgfbp1inhumanendothelialcells AT antoniodeluca mir4432targetsfgfbp1inhumanendothelialcells AT alessandromatarese mir4432targetsfgfbp1inhumanendothelialcells AT gaetanosantulli mir4432targetsfgfbp1inhumanendothelialcells |