A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinoma
zong non-coding RNAs (lncRNAs) have been identified as crucial effector in modulating the progression of assorted malignancies. In our study, the main aim was to unveil the role and the underlying regulatory mechanism of long intergenic non-protein coding RNA 1605 (LINC01605) in nasopharyngeal carci...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
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| Series: | RNA Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15476286.2022.2027149 |
| _version_ | 1797403959318544384 |
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| author | Weiguo Zhao Ling Xin Lei Tang Yunjing Li Xueqin Li Ruifeng Liu |
| author_facet | Weiguo Zhao Ling Xin Lei Tang Yunjing Li Xueqin Li Ruifeng Liu |
| author_sort | Weiguo Zhao |
| collection | DOAJ |
| description | zong non-coding RNAs (lncRNAs) have been identified as crucial effector in modulating the progression of assorted malignancies. In our study, the main aim was to unveil the role and the underlying regulatory mechanism of long intergenic non-protein coding RNA 1605 (LINC01605) in nasopharyngeal carcinoma (NPC). RT-qPCR analysis results suggested that LINC01605 was upregulated in NPC cells. According to the results of function experiments, LINC01605 promoted NPC cell proliferation and impeded cell apoptosis. The oncogenic role of LINC01605 in NPC was further validated by animal experiments. Additionally, we verified that LINC01605 regulated Ikbkb expression to promote the nuclear translocation of p65 and thereby activated the NF-κB pathway in NPC cells. Mechanism experiments further suggested that LINC01605 could regulate Ikbkb expression via sponging miR-942-5p. Moreover, LINC01605 recruited IGF2BP2 to stabilize ubiquitin-specific protease 3 (USP3) mRNA and thereby enhanced the stability of IkB subunit beta (IKKβ) protein. In addition, p65 acted as a transcription activator to upregulate LINC01605 in NPC cells. In conclusion, this study demonstrated a positive feedback loop between LINC01605 and the NF-κB signalling pathway that promoted NPC cell growth, thus providing new insights to better understand NPC. |
| first_indexed | 2024-03-09T02:47:11Z |
| format | Article |
| id | doaj.art-445aa1584e4b46fd9a89c802739eac27 |
| institution | Directory Open Access Journal |
| issn | 1547-6286 1555-8584 |
| language | English |
| last_indexed | 2024-03-09T02:47:11Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | RNA Biology |
| spelling | doaj.art-445aa1584e4b46fd9a89c802739eac272023-12-05T16:09:51ZengTaylor & Francis GroupRNA Biology1547-62861555-85842022-12-0119148249510.1080/15476286.2022.20271492027149A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinomaWeiguo Zhao0Ling Xin1Lei Tang2Yunjing Li3Xueqin Li4Ruifeng Liu5Zhongshan Hospital Affiliated to Sun Yat-Sen UniversityZhongshan Hospital Affiliated to Sun Yat-Sen UniversityZhongshan Hospital Affiliated to Sun Yat-Sen UniversityZhongshan Hospital Affiliated to Sun Yat-Sen UniversityZhongshan Hospital Affiliated to Sun Yat-Sen UniversityZhongshan Hospital Affiliated to Sun Yat-Sen Universityzong non-coding RNAs (lncRNAs) have been identified as crucial effector in modulating the progression of assorted malignancies. In our study, the main aim was to unveil the role and the underlying regulatory mechanism of long intergenic non-protein coding RNA 1605 (LINC01605) in nasopharyngeal carcinoma (NPC). RT-qPCR analysis results suggested that LINC01605 was upregulated in NPC cells. According to the results of function experiments, LINC01605 promoted NPC cell proliferation and impeded cell apoptosis. The oncogenic role of LINC01605 in NPC was further validated by animal experiments. Additionally, we verified that LINC01605 regulated Ikbkb expression to promote the nuclear translocation of p65 and thereby activated the NF-κB pathway in NPC cells. Mechanism experiments further suggested that LINC01605 could regulate Ikbkb expression via sponging miR-942-5p. Moreover, LINC01605 recruited IGF2BP2 to stabilize ubiquitin-specific protease 3 (USP3) mRNA and thereby enhanced the stability of IkB subunit beta (IKKβ) protein. In addition, p65 acted as a transcription activator to upregulate LINC01605 in NPC cells. In conclusion, this study demonstrated a positive feedback loop between LINC01605 and the NF-κB signalling pathway that promoted NPC cell growth, thus providing new insights to better understand NPC.http://dx.doi.org/10.1080/15476286.2022.2027149linc01605nf-κb pathwaynasopharyngeal carcinomap65 |
| spellingShingle | Weiguo Zhao Ling Xin Lei Tang Yunjing Li Xueqin Li Ruifeng Liu A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinoma RNA Biology linc01605 nf-κb pathway nasopharyngeal carcinoma p65 |
| title | A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinoma |
| title_full | A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinoma |
| title_fullStr | A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinoma |
| title_full_unstemmed | A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinoma |
| title_short | A positive feedback loop between LINC01605 and NF-κB pathway promotes tumor growth in nasopharyngeal carcinoma |
| title_sort | positive feedback loop between linc01605 and nf κb pathway promotes tumor growth in nasopharyngeal carcinoma |
| topic | linc01605 nf-κb pathway nasopharyngeal carcinoma p65 |
| url | http://dx.doi.org/10.1080/15476286.2022.2027149 |
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