Antibacterial Activity of Propolis Extracts from the Central Region of Romania against <i>Neisseria gonorrhoeae</i>
(1) Background: Sexually transmitted infections (STIs) are among the most common infections worldwide, many of these being caused by <i>Neisseria gonorrhoeae</i> (NG). Increased antimicrobial NG resistance has been reported in recent decades, highlighting the need for new sources of natu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/6/689 |
| _version_ | 1827690272136364032 |
|---|---|
| author | Mihaela Laura Vică Ioana Glevitzky Mirel Glevitzky Costel Vasile Siserman Horea Vladi Matei Cosmin Adrian Teodoru |
| author_facet | Mihaela Laura Vică Ioana Glevitzky Mirel Glevitzky Costel Vasile Siserman Horea Vladi Matei Cosmin Adrian Teodoru |
| author_sort | Mihaela Laura Vică |
| collection | DOAJ |
| description | (1) Background: Sexually transmitted infections (STIs) are among the most common infections worldwide, many of these being caused by <i>Neisseria gonorrhoeae</i> (NG). Increased antimicrobial NG resistance has been reported in recent decades, highlighting the need for new sources of natural compounds with valuable antimicrobial activity. This study aims to determine the effect of propolis extracts on NG strains, including antibiotic-resistant strains. (2) Methods: First void urine samples from presumed positive STI subjects were harvested. DNA was extracted, purified, and amplified via PCR for the simultaneous detection of 6 STIs. The presence of the <i>dcmH</i>, <i>gyrA</i>, and <i>parC</i> genes was checked in the DNA samples from NG-positive patients. The antimicrobial activity of 5 aqueous propolis extracts from central Romania was investigated in vitro against some isolated NG strains. ANOVA tests were employed to assess differences and interactions between the inhibition zone for NG strains and propolis extracts. (3) Results: 7.07% of the patients presented NG infections, some strains being resistant or intermediate-resistant to ciprofloxacin. All propolis samples exhibited an antibacterial effect, including on resistant strains. (4) Conclusions: Statistical analysis demonstrated that the diameter of the inhibition zone was influenced both by the NG strain type and the source of the propolis extracts. |
| first_indexed | 2024-03-10T10:36:00Z |
| format | Article |
| id | doaj.art-445acbbd99254b479c25ed24a746f1e9 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T10:36:00Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-445acbbd99254b479c25ed24a746f1e92023-11-21T23:17:18ZengMDPI AGAntibiotics2079-63822021-06-0110668910.3390/antibiotics10060689Antibacterial Activity of Propolis Extracts from the Central Region of Romania against <i>Neisseria gonorrhoeae</i>Mihaela Laura Vică0Ioana Glevitzky1Mirel Glevitzky2Costel Vasile Siserman3Horea Vladi Matei4Cosmin Adrian Teodoru5Department of Cellular and Molecular Biology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDoctoral School, Faculty of Engineering, “Lucian Blaga” University of Sibiu, 550025 Sibiu, RomaniaFaculty of Exact Science and Engineering, “1 Decembrie 1918” University of Alba Iulia, 510009 Alba Iulia, RomaniaDepartment of Legal Medicine, ‘Iuliu Haţieganu’ University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Cellular and Molecular Biology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaClinical Surgical Department, Faculty of Medicine, “Lucian Blaga” University, 550002 Sibiu, Romania(1) Background: Sexually transmitted infections (STIs) are among the most common infections worldwide, many of these being caused by <i>Neisseria gonorrhoeae</i> (NG). Increased antimicrobial NG resistance has been reported in recent decades, highlighting the need for new sources of natural compounds with valuable antimicrobial activity. This study aims to determine the effect of propolis extracts on NG strains, including antibiotic-resistant strains. (2) Methods: First void urine samples from presumed positive STI subjects were harvested. DNA was extracted, purified, and amplified via PCR for the simultaneous detection of 6 STIs. The presence of the <i>dcmH</i>, <i>gyrA</i>, and <i>parC</i> genes was checked in the DNA samples from NG-positive patients. The antimicrobial activity of 5 aqueous propolis extracts from central Romania was investigated in vitro against some isolated NG strains. ANOVA tests were employed to assess differences and interactions between the inhibition zone for NG strains and propolis extracts. (3) Results: 7.07% of the patients presented NG infections, some strains being resistant or intermediate-resistant to ciprofloxacin. All propolis samples exhibited an antibacterial effect, including on resistant strains. (4) Conclusions: Statistical analysis demonstrated that the diameter of the inhibition zone was influenced both by the NG strain type and the source of the propolis extracts.https://www.mdpi.com/2079-6382/10/6/689<i>Neisseria gonorrhoeae</i>ciprofloxacin resistanceantibacterial activitypropolis extracts |
| spellingShingle | Mihaela Laura Vică Ioana Glevitzky Mirel Glevitzky Costel Vasile Siserman Horea Vladi Matei Cosmin Adrian Teodoru Antibacterial Activity of Propolis Extracts from the Central Region of Romania against <i>Neisseria gonorrhoeae</i> Antibiotics <i>Neisseria gonorrhoeae</i> ciprofloxacin resistance antibacterial activity propolis extracts |
| title | Antibacterial Activity of Propolis Extracts from the Central Region of Romania against <i>Neisseria gonorrhoeae</i> |
| title_full | Antibacterial Activity of Propolis Extracts from the Central Region of Romania against <i>Neisseria gonorrhoeae</i> |
| title_fullStr | Antibacterial Activity of Propolis Extracts from the Central Region of Romania against <i>Neisseria gonorrhoeae</i> |
| title_full_unstemmed | Antibacterial Activity of Propolis Extracts from the Central Region of Romania against <i>Neisseria gonorrhoeae</i> |
| title_short | Antibacterial Activity of Propolis Extracts from the Central Region of Romania against <i>Neisseria gonorrhoeae</i> |
| title_sort | antibacterial activity of propolis extracts from the central region of romania against i neisseria gonorrhoeae i |
| topic | <i>Neisseria gonorrhoeae</i> ciprofloxacin resistance antibacterial activity propolis extracts |
| url | https://www.mdpi.com/2079-6382/10/6/689 |
| work_keys_str_mv | AT mihaelalauravica antibacterialactivityofpropolisextractsfromthecentralregionofromaniaagainstineisseriagonorrhoeaei AT ioanaglevitzky antibacterialactivityofpropolisextractsfromthecentralregionofromaniaagainstineisseriagonorrhoeaei AT mirelglevitzky antibacterialactivityofpropolisextractsfromthecentralregionofromaniaagainstineisseriagonorrhoeaei AT costelvasilesiserman antibacterialactivityofpropolisextractsfromthecentralregionofromaniaagainstineisseriagonorrhoeaei AT horeavladimatei antibacterialactivityofpropolisextractsfromthecentralregionofromaniaagainstineisseriagonorrhoeaei AT cosminadrianteodoru antibacterialactivityofpropolisextractsfromthecentralregionofromaniaagainstineisseriagonorrhoeaei |