Systematic identification of cell size regulators in budding yeast
Abstract Cell size is determined by a complex interplay between growth and division, involving multiple cellular pathways. To identify systematically processes affecting size control in G1 in budding yeast, we imaged and analyzed the cell cycle of millions of individual cells representing 591 mutant...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2014-11-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.20145345 |
| _version_ | 1797279806800265216 |
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| author | Ilya Soifer Naama Barkai |
| author_facet | Ilya Soifer Naama Barkai |
| author_sort | Ilya Soifer |
| collection | DOAJ |
| description | Abstract Cell size is determined by a complex interplay between growth and division, involving multiple cellular pathways. To identify systematically processes affecting size control in G1 in budding yeast, we imaged and analyzed the cell cycle of millions of individual cells representing 591 mutants implicated in size control. Quantitative metric distinguished mutants affecting the mechanism of size control from the majority of mutants that have a perturbed size due to indirect effects modulating cell growth. Overall, we identified 17 negative and dozens positive size control regulators, with the negative regulators forming a small network centered on elements of mitotic exit network. Some elements of the translation machinery affected size control with a notable distinction between the deletions of parts of small and large ribosomal subunit: parts of small ribosomal subunit tended to regulate size control, while parts of the large subunit affected cell growth. Analysis of small cells revealed additional size control mechanism that functions in G2/M, complementing the primary size control in G1. Our study provides new insights about size control mechanisms in budding yeast. |
| first_indexed | 2024-03-07T16:31:22Z |
| format | Article |
| id | doaj.art-445d8caa880c4aa79aa1e36e32acff29 |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2024-03-07T16:31:22Z |
| publishDate | 2014-11-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-445d8caa880c4aa79aa1e36e32acff292024-03-03T10:19:58ZengSpringer NatureMolecular Systems Biology1744-42922014-11-011011n/an/a10.15252/msb.20145345Systematic identification of cell size regulators in budding yeastIlya Soifer0Naama Barkai1Department of Molecular Genetics Weizmann Institute of Science Rehovot IsraelDepartment of Molecular Genetics Weizmann Institute of Science Rehovot IsraelAbstract Cell size is determined by a complex interplay between growth and division, involving multiple cellular pathways. To identify systematically processes affecting size control in G1 in budding yeast, we imaged and analyzed the cell cycle of millions of individual cells representing 591 mutants implicated in size control. Quantitative metric distinguished mutants affecting the mechanism of size control from the majority of mutants that have a perturbed size due to indirect effects modulating cell growth. Overall, we identified 17 negative and dozens positive size control regulators, with the negative regulators forming a small network centered on elements of mitotic exit network. Some elements of the translation machinery affected size control with a notable distinction between the deletions of parts of small and large ribosomal subunit: parts of small ribosomal subunit tended to regulate size control, while parts of the large subunit affected cell growth. Analysis of small cells revealed additional size control mechanism that functions in G2/M, complementing the primary size control in G1. Our study provides new insights about size control mechanisms in budding yeast.https://doi.org/10.15252/msb.20145345cell growthsize controlSTARTyeast genetics |
| spellingShingle | Ilya Soifer Naama Barkai Systematic identification of cell size regulators in budding yeast Molecular Systems Biology cell growth size control START yeast genetics |
| title | Systematic identification of cell size regulators in budding yeast |
| title_full | Systematic identification of cell size regulators in budding yeast |
| title_fullStr | Systematic identification of cell size regulators in budding yeast |
| title_full_unstemmed | Systematic identification of cell size regulators in budding yeast |
| title_short | Systematic identification of cell size regulators in budding yeast |
| title_sort | systematic identification of cell size regulators in budding yeast |
| topic | cell growth size control START yeast genetics |
| url | https://doi.org/10.15252/msb.20145345 |
| work_keys_str_mv | AT ilyasoifer systematicidentificationofcellsizeregulatorsinbuddingyeast AT naamabarkai systematicidentificationofcellsizeregulatorsinbuddingyeast |