Antibacterial Activity of <i>Boswellia sacra</i> Flueck. Oleoresin Extract against <i>Porphyromonas gingivalis</i> Periodontal Pathogen
<i>Boswellia sacra</i> Flueck. oleoresin extract (frankincense) has traditionally been used in the treatment of different diseases, but there are no sufficient studies on its potential activity against periodontal pathogens. Therefore, antibacterial and antibiofilm activity of frankincen...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/7/859 |
| _version_ | 1797527721188786176 |
|---|---|
| author | Nashwah G. M. Attallah Walaa A. Negm Engy Elekhnawy Najla Altwaijry Elshaymaa I. Elmongy Thanaa A. El-Masry Eman A. Alturki Doaa A. Yousef Malak Y. Shoukheba |
| author_facet | Nashwah G. M. Attallah Walaa A. Negm Engy Elekhnawy Najla Altwaijry Elshaymaa I. Elmongy Thanaa A. El-Masry Eman A. Alturki Doaa A. Yousef Malak Y. Shoukheba |
| author_sort | Nashwah G. M. Attallah |
| collection | DOAJ |
| description | <i>Boswellia sacra</i> Flueck. oleoresin extract (frankincense) has traditionally been used in the treatment of different diseases, but there are no sufficient studies on its potential activity against periodontal pathogens. Therefore, antibacterial and antibiofilm activity of frankincense extract against <i>Porphyromonas gingivalis</i> clinical isolates were studied. The phytochemical composition of the volatile components of the extract was identified by GC-MS analysis revealing 49 compounds as <i>trans</i>-nerolidyl formate, cycloartenol acetate, ursenoic acid 3-oxomethyl ester, bisabolene epoxide, and kaur-16-ene. It decreased the growth and increased the leakage of nucleotides in 58.3% and 33.3% of isolates, respectively. Additionally, it reduced the extracellular polysaccharide production and the cell surface hydrophobicity in 41.67% and 50% of the isolates, respectively. Crystal violet assay revealed inhibition of biofilm formation by the tested isolates. Light microscope and scanning electron microscope were used to examine the biofilms and they confirmed the reduction of biofilm formation by frankincense extract. Downregulation of the genes linked to biofilm formation (<i>fim</i>A, <i>hag</i>A, and <i>hag</i>B) was observed using qRT-PCR after treatment with the frankincense extract. This study suggested that the frankincense extract could exhibit antibacterial and antibiofilm activity against <i>P. gingivalis</i> isolates. Thus, the frankincense extract could be used as a treatment approach for periodontitis. |
| first_indexed | 2024-03-10T09:47:48Z |
| format | Article |
| id | doaj.art-4460a52b361d41558dfd21b72821e9ca |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:47:48Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-4460a52b361d41558dfd21b72821e9ca2023-11-22T03:04:24ZengMDPI AGAntibiotics2079-63822021-07-0110785910.3390/antibiotics10070859Antibacterial Activity of <i>Boswellia sacra</i> Flueck. Oleoresin Extract against <i>Porphyromonas gingivalis</i> Periodontal PathogenNashwah G. M. Attallah0Walaa A. Negm1Engy Elekhnawy2Najla Altwaijry3Elshaymaa I. Elmongy4Thanaa A. El-Masry5Eman A. Alturki6Doaa A. Yousef7Malak Y. Shoukheba8Pharmaceutical Sciences Department, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh 84428, Saudi ArabiaPharmacognosy Department, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptPharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptPharmaceutical Sciences Department, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh 84428, Saudi ArabiaPharmaceutical Sciences Department, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh 84428, Saudi ArabiaPharmacology Department, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptPharmacognosy Department, College of Pharmacy, King Saud University, Riyadh 84428, Saudi ArabiaOral Medicine, Periodontology, Oral Diagnosis, and Radiology Department, Faculty of Dentistry, Tanta University, Tanta 31527, EgyptOral Medicine, Periodontology, Oral Diagnosis, and Radiology Department, Faculty of Dentistry, Tanta University, Tanta 31527, Egypt<i>Boswellia sacra</i> Flueck. oleoresin extract (frankincense) has traditionally been used in the treatment of different diseases, but there are no sufficient studies on its potential activity against periodontal pathogens. Therefore, antibacterial and antibiofilm activity of frankincense extract against <i>Porphyromonas gingivalis</i> clinical isolates were studied. The phytochemical composition of the volatile components of the extract was identified by GC-MS analysis revealing 49 compounds as <i>trans</i>-nerolidyl formate, cycloartenol acetate, ursenoic acid 3-oxomethyl ester, bisabolene epoxide, and kaur-16-ene. It decreased the growth and increased the leakage of nucleotides in 58.3% and 33.3% of isolates, respectively. Additionally, it reduced the extracellular polysaccharide production and the cell surface hydrophobicity in 41.67% and 50% of the isolates, respectively. Crystal violet assay revealed inhibition of biofilm formation by the tested isolates. Light microscope and scanning electron microscope were used to examine the biofilms and they confirmed the reduction of biofilm formation by frankincense extract. Downregulation of the genes linked to biofilm formation (<i>fim</i>A, <i>hag</i>A, and <i>hag</i>B) was observed using qRT-PCR after treatment with the frankincense extract. This study suggested that the frankincense extract could exhibit antibacterial and antibiofilm activity against <i>P. gingivalis</i> isolates. Thus, the frankincense extract could be used as a treatment approach for periodontitis.https://www.mdpi.com/2079-6382/10/7/859biofilmchronic periodontitisextracellular polysaccharidesGC-MSqRT-PCRscanning electron microscope |
| spellingShingle | Nashwah G. M. Attallah Walaa A. Negm Engy Elekhnawy Najla Altwaijry Elshaymaa I. Elmongy Thanaa A. El-Masry Eman A. Alturki Doaa A. Yousef Malak Y. Shoukheba Antibacterial Activity of <i>Boswellia sacra</i> Flueck. Oleoresin Extract against <i>Porphyromonas gingivalis</i> Periodontal Pathogen Antibiotics biofilm chronic periodontitis extracellular polysaccharides GC-MS qRT-PCR scanning electron microscope |
| title | Antibacterial Activity of <i>Boswellia sacra</i> Flueck. Oleoresin Extract against <i>Porphyromonas gingivalis</i> Periodontal Pathogen |
| title_full | Antibacterial Activity of <i>Boswellia sacra</i> Flueck. Oleoresin Extract against <i>Porphyromonas gingivalis</i> Periodontal Pathogen |
| title_fullStr | Antibacterial Activity of <i>Boswellia sacra</i> Flueck. Oleoresin Extract against <i>Porphyromonas gingivalis</i> Periodontal Pathogen |
| title_full_unstemmed | Antibacterial Activity of <i>Boswellia sacra</i> Flueck. Oleoresin Extract against <i>Porphyromonas gingivalis</i> Periodontal Pathogen |
| title_short | Antibacterial Activity of <i>Boswellia sacra</i> Flueck. Oleoresin Extract against <i>Porphyromonas gingivalis</i> Periodontal Pathogen |
| title_sort | antibacterial activity of i boswellia sacra i flueck oleoresin extract against i porphyromonas gingivalis i periodontal pathogen |
| topic | biofilm chronic periodontitis extracellular polysaccharides GC-MS qRT-PCR scanning electron microscope |
| url | https://www.mdpi.com/2079-6382/10/7/859 |
| work_keys_str_mv | AT nashwahgmattallah antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen AT walaaanegm antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen AT engyelekhnawy antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen AT najlaaltwaijry antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen AT elshaymaaielmongy antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen AT thanaaaelmasry antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen AT emanaalturki antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen AT doaaayousef antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen AT malakyshoukheba antibacterialactivityofiboswelliasacraiflueckoleoresinextractagainstiporphyromonasgingivalisiperiodontalpathogen |