The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions
The anti-Müllerian hormone (AMH) is a glycoprotein that plays an important role in prenatal sex differentiation. It is used as a biomarker in polycystic ovary syndrome (PCOS) diagnostics, as well as for estimating an individual’s ovarian reserve and the ovarian response to hormonal stimulation durin...
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MDPI AG
2023-04-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/8/1501 |
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| author | Radana Vrzáková Václav Šimánek Ondřej Topolčan Vladimír Vurm David Slouka Radek Kučera |
| author_facet | Radana Vrzáková Václav Šimánek Ondřej Topolčan Vladimír Vurm David Slouka Radek Kučera |
| author_sort | Radana Vrzáková |
| collection | DOAJ |
| description | The anti-Müllerian hormone (AMH) is a glycoprotein that plays an important role in prenatal sex differentiation. It is used as a biomarker in polycystic ovary syndrome (PCOS) diagnostics, as well as for estimating an individual’s ovarian reserve and the ovarian response to hormonal stimulation during in vitro fertilization (IVF). The aim of this study was to test the stability of AMH during various preanalytical conditions that are in accordance with the ISBER (International Society for Biological and Environmental Repositories) protocol. Plasma and serum samples were taken from each of the 26 participants. The samples were then processed according to the ISBER protocol. AMH levels were measured in all the samples simultaneously using the chemiluminescent kit ACCESS AMH in a UniCel<sup>®</sup> DxI 800 Immunoassay System (Beckman Coulter, Brea, CA, USA). The study proved that AMH retains a relatively high degree of stability during repeated freezing and thawing in serum. AMH was shown to be less stable in plasma samples. Room temperature proved to be the least suitable condition for the storage of samples before performing the biomarker analysis. During the testing of storage stability at 5–7 °C, the values decreased over time for all the plasma samples but remained stable in the serum samples. We proved that AMH is highly stable under various stress conditions. The anti-Müllerian hormone retained the greatest stability in the serum samples. |
| first_indexed | 2024-03-11T05:05:52Z |
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| id | doaj.art-446be1a1b7de4f1ca0a05f52506ba211 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T05:05:52Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-446be1a1b7de4f1ca0a05f52506ba2112023-11-17T18:56:03ZengMDPI AGDiagnostics2075-44182023-04-01138150110.3390/diagnostics13081501The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical ConditionsRadana Vrzáková0Václav Šimánek1Ondřej Topolčan2Vladimír Vurm3David Slouka4Radek Kučera5Department of Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech RepublicDepartment of Immunochemistry, University Hospital and Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech RepublicDepartment of Immunochemistry, University Hospital and Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech RepublicDepartment of Immunochemistry, University Hospital and Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech RepublicDepartment of Otorhinolaryngology, University Hospital and Faculty of Medicine in Pilsen, Charles University, 30955 Pilsen, Czech RepublicDepartment of Immunochemistry, University Hospital and Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech RepublicThe anti-Müllerian hormone (AMH) is a glycoprotein that plays an important role in prenatal sex differentiation. It is used as a biomarker in polycystic ovary syndrome (PCOS) diagnostics, as well as for estimating an individual’s ovarian reserve and the ovarian response to hormonal stimulation during in vitro fertilization (IVF). The aim of this study was to test the stability of AMH during various preanalytical conditions that are in accordance with the ISBER (International Society for Biological and Environmental Repositories) protocol. Plasma and serum samples were taken from each of the 26 participants. The samples were then processed according to the ISBER protocol. AMH levels were measured in all the samples simultaneously using the chemiluminescent kit ACCESS AMH in a UniCel<sup>®</sup> DxI 800 Immunoassay System (Beckman Coulter, Brea, CA, USA). The study proved that AMH retains a relatively high degree of stability during repeated freezing and thawing in serum. AMH was shown to be less stable in plasma samples. Room temperature proved to be the least suitable condition for the storage of samples before performing the biomarker analysis. During the testing of storage stability at 5–7 °C, the values decreased over time for all the plasma samples but remained stable in the serum samples. We proved that AMH is highly stable under various stress conditions. The anti-Müllerian hormone retained the greatest stability in the serum samples.https://www.mdpi.com/2075-4418/13/8/1501anti-Müllerian hormone (AMH)serumplasmapreanalytical conditionsstability |
| spellingShingle | Radana Vrzáková Václav Šimánek Ondřej Topolčan Vladimír Vurm David Slouka Radek Kučera The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions Diagnostics anti-Müllerian hormone (AMH) serum plasma preanalytical conditions stability |
| title | The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions |
| title_full | The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions |
| title_fullStr | The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions |
| title_full_unstemmed | The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions |
| title_short | The Stability of the Anti-Müllerian Hormone in Serum and Plasma Samples under Various Preanalytical Conditions |
| title_sort | stability of the anti mullerian hormone in serum and plasma samples under various preanalytical conditions |
| topic | anti-Müllerian hormone (AMH) serum plasma preanalytical conditions stability |
| url | https://www.mdpi.com/2075-4418/13/8/1501 |
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