Characterization of the development of Haemonchus contortus ZJ strain from gerbils
Abstract Background Haemonchus contortus is a serious parasitic nematode in domestic ruminants around the world, including China. Haemonchus contortus has developed extensive resistance to commercial anthelmintics, which has produced a demand for new control methods, such as more effective drugs. Ge...
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| Format: | Article |
| Language: | English |
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BMC
2017-10-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://link.springer.com/article/10.1186/s13071-017-2465-1 |
| _version_ | 1819047545392857088 |
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| author | Yi Yang Xiaolu Guo Hongli Zhang Yan Huang Xueqiu Chen Aifang Du |
| author_facet | Yi Yang Xiaolu Guo Hongli Zhang Yan Huang Xueqiu Chen Aifang Du |
| author_sort | Yi Yang |
| collection | DOAJ |
| description | Abstract Background Haemonchus contortus is a serious parasitic nematode in domestic ruminants around the world, including China. Haemonchus contortus has developed extensive resistance to commercial anthelmintics, which has produced a demand for new control methods, such as more effective drugs. Gerbils infected with H. contortus have previously been used as a model for anthelmintics selections, and the growth of H. contortus had been briefly examined. To enhance the model, this study provides an additional description of the development of H. contortus ZJ strain in gerbils. Results Gerbils were infected with H. contortus ZJ strain at a dose of 2000 exsheathed infective larvae (xL3s) and sacrificed at 4, 7 and 18 days post-infection (dpi). Only fourth-stage larvae were found in the stomachs. About 2% of the inoculums were obtained at each of the three sampling time points. Larvae grew more slowly in gerbils than in sheep, but presented almost the same morphology. Rod-like crystalline inclusions were present in the intestinal cells of larvae, indicating that the metabolic rate of larvae was probably greatly reduced. Histological examination of stomach sections showed that larvae are located in the lumens or at the mucosal surfaces, with few inflammatory changes evident. Conclusions The development and features of H. contortus ZJ strain in gerbils were described. Our results provide supplementary information of H. contortus growth in gerbils, especially the presence of rod-like crystalline inclusions, and may contribute to improve the anthelmintic selection system. |
| first_indexed | 2024-12-21T11:02:04Z |
| format | Article |
| id | doaj.art-447390fe78744de0bfad48f9d1e76533 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-21T11:02:04Z |
| publishDate | 2017-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-447390fe78744de0bfad48f9d1e765332022-12-21T19:06:20ZengBMCParasites & Vectors1756-33052017-10-011011610.1186/s13071-017-2465-1Characterization of the development of Haemonchus contortus ZJ strain from gerbilsYi Yang0Xiaolu Guo1Hongli Zhang2Yan Huang3Xueqiu Chen4Aifang Du5Institute of Preventive Veterinary Medicine, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang UniversityInstitute of Preventive Veterinary Medicine, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang UniversityZhejiang Center for Animal Disease Control and PreventionInstitute of Preventive Veterinary Medicine, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang UniversityInstitute of Preventive Veterinary Medicine, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang UniversityInstitute of Preventive Veterinary Medicine, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang UniversityAbstract Background Haemonchus contortus is a serious parasitic nematode in domestic ruminants around the world, including China. Haemonchus contortus has developed extensive resistance to commercial anthelmintics, which has produced a demand for new control methods, such as more effective drugs. Gerbils infected with H. contortus have previously been used as a model for anthelmintics selections, and the growth of H. contortus had been briefly examined. To enhance the model, this study provides an additional description of the development of H. contortus ZJ strain in gerbils. Results Gerbils were infected with H. contortus ZJ strain at a dose of 2000 exsheathed infective larvae (xL3s) and sacrificed at 4, 7 and 18 days post-infection (dpi). Only fourth-stage larvae were found in the stomachs. About 2% of the inoculums were obtained at each of the three sampling time points. Larvae grew more slowly in gerbils than in sheep, but presented almost the same morphology. Rod-like crystalline inclusions were present in the intestinal cells of larvae, indicating that the metabolic rate of larvae was probably greatly reduced. Histological examination of stomach sections showed that larvae are located in the lumens or at the mucosal surfaces, with few inflammatory changes evident. Conclusions The development and features of H. contortus ZJ strain in gerbils were described. Our results provide supplementary information of H. contortus growth in gerbils, especially the presence of rod-like crystalline inclusions, and may contribute to improve the anthelmintic selection system.http://link.springer.com/article/10.1186/s13071-017-2465-1Haemonchus contortus ZJ strainGerbilDevelopmentRod-like crystalline inclusions |
| spellingShingle | Yi Yang Xiaolu Guo Hongli Zhang Yan Huang Xueqiu Chen Aifang Du Characterization of the development of Haemonchus contortus ZJ strain from gerbils Parasites & Vectors Haemonchus contortus ZJ strain Gerbil Development Rod-like crystalline inclusions |
| title | Characterization of the development of Haemonchus contortus ZJ strain from gerbils |
| title_full | Characterization of the development of Haemonchus contortus ZJ strain from gerbils |
| title_fullStr | Characterization of the development of Haemonchus contortus ZJ strain from gerbils |
| title_full_unstemmed | Characterization of the development of Haemonchus contortus ZJ strain from gerbils |
| title_short | Characterization of the development of Haemonchus contortus ZJ strain from gerbils |
| title_sort | characterization of the development of haemonchus contortus zj strain from gerbils |
| topic | Haemonchus contortus ZJ strain Gerbil Development Rod-like crystalline inclusions |
| url | http://link.springer.com/article/10.1186/s13071-017-2465-1 |
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