A clinical case of posttraumatic osteomyelitis associated with <i>kytococcus schroeteri</i> and <i>enterococcus faecalis</i>
Introduction. In most cases, osteomyelitis is the monomicrobial disease caused by various Gram-positive bacteria. However, previous injury may contribute to formation of a microbial association. Presence of two or more pathogens in the infectious focus can markedly complicate the disease clinical pi...
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| Language: | Russian |
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Sankt-Peterburg : NIIÈM imeni Pastera
2023-11-01
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| Series: | Инфекция и иммунитет |
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| Online Access: | https://iimmun.ru/iimm/article/viewFile/15623/1783 |
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| author | Sergey D. Borisov Ilshat F. Karimov Andrey O. Plotnikov Ksenia S. Inchagova Alexandr S. Pankov Dmitry P. Danshin |
| author_facet | Sergey D. Borisov Ilshat F. Karimov Andrey O. Plotnikov Ksenia S. Inchagova Alexandr S. Pankov Dmitry P. Danshin |
| author_sort | Sergey D. Borisov |
| collection | DOAJ |
| description | Introduction. In most cases, osteomyelitis is the monomicrobial disease caused by various Gram-positive bacteria. However, previous injury may contribute to formation of a microbial association. Presence of two or more pathogens in the infectious focus can markedly complicate the disease clinical picture.Kytococcus schroeteriis one of the rarest pathogens found in clinical samples. Slightly more than twenty described cases related to such infection have been recorded globally. The aim of the research was to analyse the first identified clinical case of post-traumatic osteomyelitis associated withKytococcus schroeteriandEnterococcus faecalis.Materials and methods.The bacterial cultures were obtained from the wound discharge of the patient’s shin by the cultural method. Identification of the bacterial cultures was performed using a VITEK MS mass spectrometer followed by 16S rRNA gene sequencing. The morphological, tinctorial and biochemical features of the cultures obtained were established, and their antibacterial resistance was also determined.Results. The clinical case was associated with the diagnosis “Chronic post-traumatic osteomyelitis of the left shin”. Two types of microorganisms were isolated from the wound discharge, and identified asKytococcus schroeteriandEnterococcus faecalisusing the MALDI-ToF mass spectrometry method. 16S rRNA gene sequencing from the isolated bacterial cultures confirmed the MALDI-ToF mass spectrometry data. Antimicrobial susceptibility for this microorganism was determined according to the criteria for the staphylococcal group according to the clinical guide “Determination of sensitivity to antimicrobial drugs (rev. 2021)”.Conclusion. For the first time, the mixed infection caused by two Gram-positive bacteriaK. schroeteriandE. faecalishas been described. Such associations can enhance the pathogenic effects of each other bacterium, which may contribute to transition of the infection to a chronic form with a low probability of positive cultural test.K. schroeteriis a representative of the normal skin microbiota, but this microorganism is able to cause various infections. TheK. schroeterispecies should be differentiated from other representatives of the orderMicrococcales. At cultural examination, resistance to oxacillin is a suspicious sign indicating that the bacterial culture might be potentially assigned toK. schroeterispecies. Due to the unavailability of current biochemical tests for differentiationK. schroeterifrom related taxa, reliable identification of this species is recommended using MALDI-ToF mass spectrometry or 16S rRNA gene sequencing. |
| first_indexed | 2024-03-08T22:52:23Z |
| format | Article |
| id | doaj.art-44754d89915d45ba84f466aaba83f16f |
| institution | Directory Open Access Journal |
| issn | 2220-7619 2313-7398 |
| language | Russian |
| last_indexed | 2024-03-08T22:52:23Z |
| publishDate | 2023-11-01 |
| publisher | Sankt-Peterburg : NIIÈM imeni Pastera |
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| series | Инфекция и иммунитет |
| spelling | doaj.art-44754d89915d45ba84f466aaba83f16f2023-12-16T10:50:06ZrusSankt-Peterburg : NIIÈM imeni PasteraИнфекция и иммунитет2220-76192313-73982023-11-0113598599410.15789/2220-7619-ACC-156231342A clinical case of posttraumatic osteomyelitis associated with <i>kytococcus schroeteri</i> and <i>enterococcus faecalis</i>Sergey D. Borisov0Ilshat F. Karimov1Andrey O. Plotnikov2https://orcid.org/0000-0001-6830-4068Ksenia S. Inchagova3Alexandr S. Pankov4Dmitry P. Danshin5Orenburg State Medical University of the Ministry of Health of RussiaOrenburg State Medical University of the Ministry of Health of RussiaOrenburg State Medical University of the Ministry of Health of RussiaInstitute for Cellular and Intracellular Symbiosis of the Ural Branch of the Russian Academy of Sciences, Orenburg Federal Research Center of the UrB RASOrenburg State Medical University of the Ministry of Health of RussiaOrenburg Regional Clinical Specialized Center of Traumatology and OrthopedicsIntroduction. In most cases, osteomyelitis is the monomicrobial disease caused by various Gram-positive bacteria. However, previous injury may contribute to formation of a microbial association. Presence of two or more pathogens in the infectious focus can markedly complicate the disease clinical picture.Kytococcus schroeteriis one of the rarest pathogens found in clinical samples. Slightly more than twenty described cases related to such infection have been recorded globally. The aim of the research was to analyse the first identified clinical case of post-traumatic osteomyelitis associated withKytococcus schroeteriandEnterococcus faecalis.Materials and methods.The bacterial cultures were obtained from the wound discharge of the patient’s shin by the cultural method. Identification of the bacterial cultures was performed using a VITEK MS mass spectrometer followed by 16S rRNA gene sequencing. The morphological, tinctorial and biochemical features of the cultures obtained were established, and their antibacterial resistance was also determined.Results. The clinical case was associated with the diagnosis “Chronic post-traumatic osteomyelitis of the left shin”. Two types of microorganisms were isolated from the wound discharge, and identified asKytococcus schroeteriandEnterococcus faecalisusing the MALDI-ToF mass spectrometry method. 16S rRNA gene sequencing from the isolated bacterial cultures confirmed the MALDI-ToF mass spectrometry data. Antimicrobial susceptibility for this microorganism was determined according to the criteria for the staphylococcal group according to the clinical guide “Determination of sensitivity to antimicrobial drugs (rev. 2021)”.Conclusion. For the first time, the mixed infection caused by two Gram-positive bacteriaK. schroeteriandE. faecalishas been described. Such associations can enhance the pathogenic effects of each other bacterium, which may contribute to transition of the infection to a chronic form with a low probability of positive cultural test.K. schroeteriis a representative of the normal skin microbiota, but this microorganism is able to cause various infections. TheK. schroeterispecies should be differentiated from other representatives of the orderMicrococcales. At cultural examination, resistance to oxacillin is a suspicious sign indicating that the bacterial culture might be potentially assigned toK. schroeterispecies. Due to the unavailability of current biochemical tests for differentiationK. schroeterifrom related taxa, reliable identification of this species is recommended using MALDI-ToF mass spectrometry or 16S rRNA gene sequencing.https://iimmun.ru/iimm/article/viewFile/15623/1783kytococcus schroeterienterococcus faecaliswound infectionosteomyelitismaldi-tof mass spectrometry16s rrna gene sequencing |
| spellingShingle | Sergey D. Borisov Ilshat F. Karimov Andrey O. Plotnikov Ksenia S. Inchagova Alexandr S. Pankov Dmitry P. Danshin A clinical case of posttraumatic osteomyelitis associated with <i>kytococcus schroeteri</i> and <i>enterococcus faecalis</i> Инфекция и иммунитет kytococcus schroeteri enterococcus faecalis wound infection osteomyelitis maldi-tof mass spectrometry 16s rrna gene sequencing |
| title | A clinical case of posttraumatic osteomyelitis associated with <i>kytococcus schroeteri</i> and <i>enterococcus faecalis</i> |
| title_full | A clinical case of posttraumatic osteomyelitis associated with <i>kytococcus schroeteri</i> and <i>enterococcus faecalis</i> |
| title_fullStr | A clinical case of posttraumatic osteomyelitis associated with <i>kytococcus schroeteri</i> and <i>enterococcus faecalis</i> |
| title_full_unstemmed | A clinical case of posttraumatic osteomyelitis associated with <i>kytococcus schroeteri</i> and <i>enterococcus faecalis</i> |
| title_short | A clinical case of posttraumatic osteomyelitis associated with <i>kytococcus schroeteri</i> and <i>enterococcus faecalis</i> |
| title_sort | clinical case of posttraumatic osteomyelitis associated with i kytococcus schroeteri i and i enterococcus faecalis i |
| topic | kytococcus schroeteri enterococcus faecalis wound infection osteomyelitis maldi-tof mass spectrometry 16s rrna gene sequencing |
| url | https://iimmun.ru/iimm/article/viewFile/15623/1783 |
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