PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and Cell Behaviors
Investigating the effect of nanomedicines on cancer cell behavior in three-dimensional (3D) platforms is beneficial for evaluating and developing novel antitumor nanomedicines in vitro. While the cytotoxicity of nanomedicines on cancer cells has been widely studied on two-dimensional flat surfaces,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/6/322 |
| _version_ | 1797594052638539776 |
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| author | Wenhai Lin Yuanhao Xu Xiao Hong Stella W. Pang |
| author_facet | Wenhai Lin Yuanhao Xu Xiao Hong Stella W. Pang |
| author_sort | Wenhai Lin |
| collection | DOAJ |
| description | Investigating the effect of nanomedicines on cancer cell behavior in three-dimensional (3D) platforms is beneficial for evaluating and developing novel antitumor nanomedicines in vitro. While the cytotoxicity of nanomedicines on cancer cells has been widely studied on two-dimensional flat surfaces, there is little work using 3D confinement to assess their effects. This study aims to address this gap by applying PEGylated paclitaxel nanoparticles (PEG-PTX NPs) for the first time to treat nasopharyngeal carcinoma (NPC43) cells in 3D confinement consisting of microwells with different sizes and a glass cover. The cytotoxicity of the small molecule drug paclitaxel (PTX) and PEG-PTX NPs was studied in microwells with sizes of 50 × 50, 100 × 100, and 150 × 150 μm<sup>2</sup> both with and without a concealed top cover. The impact of microwell confinement with varying sizes and concealment on the cytotoxicity of PTX and PEG-PTX NPs was analyzed by assessing NPC43 cell viability, migration speed, and cell morphology following treatment. Overall, microwell isolation was found to suppress drug cytotoxicity, and differences were observed in the time-dependent effects of PTX and PEG-PTX NPs on NPC43 cells in isolated and concealed microenvironments. These results not only demonstrate the effect of 3D confinement on nanomedicine cytotoxicity and cell behaviors but also provide a novel method to screen anticancer drugs and evaluate cell behaviors in vitro. |
| first_indexed | 2024-03-11T02:17:12Z |
| format | Article |
| id | doaj.art-4477479dbdca41eda32a1ee0c214e570 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T02:17:12Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-4477479dbdca41eda32a1ee0c214e5702023-11-18T11:03:27ZengMDPI AGJournal of Functional Biomaterials2079-49832023-06-0114632210.3390/jfb14060322PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and Cell BehaviorsWenhai Lin0Yuanhao Xu1Xiao Hong2Stella W. Pang3Department of Electrical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Electrical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Electrical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Electrical Engineering, City University of Hong Kong, Hong Kong, ChinaInvestigating the effect of nanomedicines on cancer cell behavior in three-dimensional (3D) platforms is beneficial for evaluating and developing novel antitumor nanomedicines in vitro. While the cytotoxicity of nanomedicines on cancer cells has been widely studied on two-dimensional flat surfaces, there is little work using 3D confinement to assess their effects. This study aims to address this gap by applying PEGylated paclitaxel nanoparticles (PEG-PTX NPs) for the first time to treat nasopharyngeal carcinoma (NPC43) cells in 3D confinement consisting of microwells with different sizes and a glass cover. The cytotoxicity of the small molecule drug paclitaxel (PTX) and PEG-PTX NPs was studied in microwells with sizes of 50 × 50, 100 × 100, and 150 × 150 μm<sup>2</sup> both with and without a concealed top cover. The impact of microwell confinement with varying sizes and concealment on the cytotoxicity of PTX and PEG-PTX NPs was analyzed by assessing NPC43 cell viability, migration speed, and cell morphology following treatment. Overall, microwell isolation was found to suppress drug cytotoxicity, and differences were observed in the time-dependent effects of PTX and PEG-PTX NPs on NPC43 cells in isolated and concealed microenvironments. These results not only demonstrate the effect of 3D confinement on nanomedicine cytotoxicity and cell behaviors but also provide a novel method to screen anticancer drugs and evaluate cell behaviors in vitro.https://www.mdpi.com/2079-4983/14/6/322nanomedicinemicrowellpaclitaxelcell migration3D confinement |
| spellingShingle | Wenhai Lin Yuanhao Xu Xiao Hong Stella W. Pang PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and Cell Behaviors Journal of Functional Biomaterials nanomedicine microwell paclitaxel cell migration 3D confinement |
| title | PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and Cell Behaviors |
| title_full | PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and Cell Behaviors |
| title_fullStr | PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and Cell Behaviors |
| title_full_unstemmed | PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and Cell Behaviors |
| title_short | PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and Cell Behaviors |
| title_sort | pegylated paclitaxel nanomedicine meets 3d confinement cytotoxicity and cell behaviors |
| topic | nanomedicine microwell paclitaxel cell migration 3D confinement |
| url | https://www.mdpi.com/2079-4983/14/6/322 |
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