Methicillin resistant Staphylococcus aureus (MRSA) from community associated settings
Antibiotic resistance is a cause of concern worldwide. Community settings are important reservoir of drug resistant microorganisms like Staphylococcus aureus. The present study was to determine the prevalence, phenotypic and genotypic antibiotic resistance pattern of S. aureus isolated from differe...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2020-07-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/102321 |
| _version_ | 1797896736530759680 |
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| author | RANDHIR SINGH SIMRANPREET KAUR J S TOMAR J P S GILL |
| author_facet | RANDHIR SINGH SIMRANPREET KAUR J S TOMAR J P S GILL |
| author_sort | RANDHIR SINGH |
| collection | DOAJ |
| description |
Antibiotic resistance is a cause of concern worldwide. Community settings are important reservoir of drug resistant microorganisms like Staphylococcus aureus. The present study was to determine the prevalence, phenotypic and genotypic antibiotic resistance pattern of S. aureus isolated from different community settings of university campus. A total of 300 swab samples were collected for isolation of S. aureus from different community settings at university campus of Guru Angad Dev Veterinary and Animal Sciences University and Punjab Agriculture University, Ludhiana, India. Confirmed S. aureus isolates were further subjected to antibiotic sensitivity by Epsilometer test (E-test) and detection of antibiotic resistance genes. The prevalence of S. aureus in the community samples was 12% (36/300). Methicillin Resistant S. aureus (MRSA) contamination among community was 3.33% (10/300). Among S. aureus isolates from community samples 63.8% (23/36) and all the MRSA isolates were multidrug resistant (MDR). Five out of 10 MRSA carried SCCmec type IVa, and 4 were pvl positive gene, therefore, designated as community associated MRSA (CA-MRSA). Phenotypic resistance to antibiotics ciprofloxacin, chloramphenicol, ceftriaxone clindamycin and trimethoprim-sulfamethoxazole was 69.4% (MIC ≥32 μg/ml), 63.9% (MIC 32 μg/ ml), 16.7% (MIC 16–64 μg/ml), 16.7% (MIC 256 μg/ml) and 8.3% (MIC 12–64 μg/ml), respectively. Resistance genes blaZ, mecA, tetK, tetM, ermB and aacA-aphD were present. Presence of MRSA and MDR variant in community settings is a public health concern, as cell phone, offices telephone, computer keyboard and tap faucet are commonly shared or touched by people. Therefore, have potential to disseminate widely, not only in the community settings but also in hospitals environment, complicating treatment.
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| first_indexed | 2024-04-10T07:47:31Z |
| format | Article |
| id | doaj.art-44788db50fe8497e988da2b638064915 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:47:31Z |
| publishDate | 2020-07-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-44788db50fe8497e988da2b6380649152023-02-23T10:27:40ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272020-07-0190310.56093/ijans.v90i3.102321Methicillin resistant Staphylococcus aureus (MRSA) from community associated settingsRANDHIR SINGH0SIMRANPREET KAUR1J S TOMAR2J P S GILL3Guru Angad Dev Veterinary Animal Sciences University, Punjab 141 004 IndiaGuru Angad Dev Veterinary Animal Sciences University, Punjab 141 004 IndiaGuru Angad Dev Veterinary Animal Sciences University, Punjab 141 004 IndiaGuru Angad Dev Veterinary Animal Sciences University, Punjab 141 004 India Antibiotic resistance is a cause of concern worldwide. Community settings are important reservoir of drug resistant microorganisms like Staphylococcus aureus. The present study was to determine the prevalence, phenotypic and genotypic antibiotic resistance pattern of S. aureus isolated from different community settings of university campus. A total of 300 swab samples were collected for isolation of S. aureus from different community settings at university campus of Guru Angad Dev Veterinary and Animal Sciences University and Punjab Agriculture University, Ludhiana, India. Confirmed S. aureus isolates were further subjected to antibiotic sensitivity by Epsilometer test (E-test) and detection of antibiotic resistance genes. The prevalence of S. aureus in the community samples was 12% (36/300). Methicillin Resistant S. aureus (MRSA) contamination among community was 3.33% (10/300). Among S. aureus isolates from community samples 63.8% (23/36) and all the MRSA isolates were multidrug resistant (MDR). Five out of 10 MRSA carried SCCmec type IVa, and 4 were pvl positive gene, therefore, designated as community associated MRSA (CA-MRSA). Phenotypic resistance to antibiotics ciprofloxacin, chloramphenicol, ceftriaxone clindamycin and trimethoprim-sulfamethoxazole was 69.4% (MIC ≥32 μg/ml), 63.9% (MIC 32 μg/ ml), 16.7% (MIC 16–64 μg/ml), 16.7% (MIC 256 μg/ml) and 8.3% (MIC 12–64 μg/ml), respectively. Resistance genes blaZ, mecA, tetK, tetM, ermB and aacA-aphD were present. Presence of MRSA and MDR variant in community settings is a public health concern, as cell phone, offices telephone, computer keyboard and tap faucet are commonly shared or touched by people. Therefore, have potential to disseminate widely, not only in the community settings but also in hospitals environment, complicating treatment. https://epubs.icar.org.in/index.php/IJAnS/article/view/102321Antibiotic resistance (AR)Antibiotic resistance genesCommunityEpsilometer testMIC valuesMultidrug resistance (MDR) |
| spellingShingle | RANDHIR SINGH SIMRANPREET KAUR J S TOMAR J P S GILL Methicillin resistant Staphylococcus aureus (MRSA) from community associated settings Indian Journal of Animal Sciences Antibiotic resistance (AR) Antibiotic resistance genes Community Epsilometer test MIC values Multidrug resistance (MDR) |
| title | Methicillin resistant Staphylococcus aureus (MRSA) from community associated settings |
| title_full | Methicillin resistant Staphylococcus aureus (MRSA) from community associated settings |
| title_fullStr | Methicillin resistant Staphylococcus aureus (MRSA) from community associated settings |
| title_full_unstemmed | Methicillin resistant Staphylococcus aureus (MRSA) from community associated settings |
| title_short | Methicillin resistant Staphylococcus aureus (MRSA) from community associated settings |
| title_sort | methicillin resistant staphylococcus aureus mrsa from community associated settings |
| topic | Antibiotic resistance (AR) Antibiotic resistance genes Community Epsilometer test MIC values Multidrug resistance (MDR) |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/102321 |
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