Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia
Saint Louis encephalitis virus (SLEV) is a member of the Japanese-encephalitis virus serocomplex of the genus Flavivirus. SLEV is broadly distributed in the Americas and the Caribbean Islands, where it is usually transmitted by mosquitoes of the genus Culex and primarily to birds and mammalian-hosts...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2015-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600719&lng=en&tlng=en |
| _version_ | 1797718071240032256 |
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| author | Richard Hoyos-López Sandra Uribe Soto Guillermo Rúa-Uribe Juan Carlos Gallego-Gómez |
| author_facet | Richard Hoyos-López Sandra Uribe Soto Guillermo Rúa-Uribe Juan Carlos Gallego-Gómez |
| author_sort | Richard Hoyos-López |
| collection | DOAJ |
| description | Saint Louis encephalitis virus (SLEV) is a member of the Japanese-encephalitis virus serocomplex of the genus Flavivirus. SLEV is broadly distributed in the Americas and the Caribbean Islands, where it is usually transmitted by mosquitoes of the genus Culex and primarily to birds and mammalian-hosts. Humans are occasionally infected by the virus and are dead-end hosts. SLEV causes encephalitis in temperate regions, while in tropical regions of the Americas, several human cases and a wide biological diversity of SLEV-strains have been reported. The phylogenetic analysis of the envelope (E) protein genes indicated eight-genotypes of SLEV with geographic overlap. The present paper describes the genotyping of two SLEV viruses detected in mosquito-pools collected in northern Colombia (department of Cordoba). We used reverse transcription-polymerase chain reaction to amplify a fragment of theE-gene to confirm the virus identity and completeE-gene sequencing for phylogenetic analysis and genotyping of the two-SLEV viruses found circulating in Córdoba. This is the first report of SLEV genotype IV in Colombia (Córdoba) in mosquitoes from a region of human inhabitation, implicating the risk of human disease due to SLEV infection. Physicians should consider SLEV as a possible aetiology for undiagnosed febrile and neurologic syndromes among their patients who report exposure to mosquito-bites. |
| first_indexed | 2024-03-12T08:45:41Z |
| format | Article |
| id | doaj.art-44792eaadff14e33a46d6d078e75bebf |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:45:41Z |
| publishDate | 2015-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-44792eaadff14e33a46d6d078e75bebf2023-09-02T16:28:30ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-09-01110671972510.1590/0074-02760280040S0074-02762015000600719Molecular identification of Saint Louis encephalitis virus genotype IV in ColombiaRichard Hoyos-LópezSandra Uribe SotoGuillermo Rúa-UribeJuan Carlos Gallego-GómezSaint Louis encephalitis virus (SLEV) is a member of the Japanese-encephalitis virus serocomplex of the genus Flavivirus. SLEV is broadly distributed in the Americas and the Caribbean Islands, where it is usually transmitted by mosquitoes of the genus Culex and primarily to birds and mammalian-hosts. Humans are occasionally infected by the virus and are dead-end hosts. SLEV causes encephalitis in temperate regions, while in tropical regions of the Americas, several human cases and a wide biological diversity of SLEV-strains have been reported. The phylogenetic analysis of the envelope (E) protein genes indicated eight-genotypes of SLEV with geographic overlap. The present paper describes the genotyping of two SLEV viruses detected in mosquito-pools collected in northern Colombia (department of Cordoba). We used reverse transcription-polymerase chain reaction to amplify a fragment of theE-gene to confirm the virus identity and completeE-gene sequencing for phylogenetic analysis and genotyping of the two-SLEV viruses found circulating in Córdoba. This is the first report of SLEV genotype IV in Colombia (Córdoba) in mosquitoes from a region of human inhabitation, implicating the risk of human disease due to SLEV infection. Physicians should consider SLEV as a possible aetiology for undiagnosed febrile and neurologic syndromes among their patients who report exposure to mosquito-bites.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600719&lng=en&tlng=enSaint Louis encephalitis virusflavivirusgenotypeColombia |
| spellingShingle | Richard Hoyos-López Sandra Uribe Soto Guillermo Rúa-Uribe Juan Carlos Gallego-Gómez Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia Memorias do Instituto Oswaldo Cruz Saint Louis encephalitis virus flavivirus genotype Colombia |
| title | Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia |
| title_full | Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia |
| title_fullStr | Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia |
| title_full_unstemmed | Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia |
| title_short | Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia |
| title_sort | molecular identification of saint louis encephalitis virus genotype iv in colombia |
| topic | Saint Louis encephalitis virus flavivirus genotype Colombia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600719&lng=en&tlng=en |
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