Gene expression analyses reveal differences in children’s response to malaria according to their age
Abstract In Bandiagara, Mali, children experience on average two clinical malaria episodes per year. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, can vary dramatically among children. We simultaneously characterize host and p...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-03-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-46416-3 |
| _version_ | 1827321350559105024 |
|---|---|
| author | Kieran Tebben Salif Yirampo Drissa Coulibaly Abdoulaye K. Koné Matthew B. Laurens Emily M. Stucke Ahmadou Dembélé Youssouf Tolo Karim Traoré Amadou Niangaly Andrea A. Berry Bourema Kouriba Christopher V. Plowe Ogobara K. Doumbo Kirsten E. Lyke Shannon Takala-Harrison Mahamadou A. Thera Mark A. Travassos David Serre |
| author_facet | Kieran Tebben Salif Yirampo Drissa Coulibaly Abdoulaye K. Koné Matthew B. Laurens Emily M. Stucke Ahmadou Dembélé Youssouf Tolo Karim Traoré Amadou Niangaly Andrea A. Berry Bourema Kouriba Christopher V. Plowe Ogobara K. Doumbo Kirsten E. Lyke Shannon Takala-Harrison Mahamadou A. Thera Mark A. Travassos David Serre |
| author_sort | Kieran Tebben |
| collection | DOAJ |
| description | Abstract In Bandiagara, Mali, children experience on average two clinical malaria episodes per year. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, can vary dramatically among children. We simultaneously characterize host and parasite gene expression profiles from 136 Malian children with symptomatic falciparum malaria and examine differences in the relative proportion of immune cells and parasite stages, as well as in gene expression, associated with infection and or patient characteristics. Parasitemia explains much of the variation in host and parasite gene expression, and infections with higher parasitemia display proportionally more neutrophils and fewer T cells, suggesting parasitemia-dependent neutrophil recruitment and/or T cell extravasation to secondary lymphoid organs. The child’s age also strongly correlates with variations in gene expression: Plasmodium falciparum genes associated with age suggest that older children carry more male gametocytes, while variations in host gene expression indicate a stronger innate response in younger children and stronger adaptive response in older children. These analyses highlight the variability in host responses and parasite regulation during P. falciparum symptomatic infections and emphasize the importance of considering the children’s age when studying and treating malaria infections. |
| first_indexed | 2024-04-25T01:04:45Z |
| format | Article |
| id | doaj.art-447cb3bdd8454f21a2e4e8996ab940e3 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-25T01:04:45Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-447cb3bdd8454f21a2e4e8996ab940e32024-03-10T12:16:13ZengNature PortfolioNature Communications2041-17232024-03-0115111410.1038/s41467-024-46416-3Gene expression analyses reveal differences in children’s response to malaria according to their ageKieran Tebben0Salif Yirampo1Drissa Coulibaly2Abdoulaye K. Koné3Matthew B. Laurens4Emily M. Stucke5Ahmadou Dembélé6Youssouf Tolo7Karim Traoré8Amadou Niangaly9Andrea A. Berry10Bourema Kouriba11Christopher V. Plowe12Ogobara K. Doumbo13Kirsten E. Lyke14Shannon Takala-Harrison15Mahamadou A. Thera16Mark A. Travassos17David Serre18Institute for Genome Sciences, University of Maryland School of MedicineMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of MedicineMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of MedicineMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of MedicineMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of MedicineMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of MedicineMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of MedicineMalaria Research and Training Center, University of Sciences, Techniques and TechnologiesMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of MedicineInstitute for Genome Sciences, University of Maryland School of MedicineAbstract In Bandiagara, Mali, children experience on average two clinical malaria episodes per year. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, can vary dramatically among children. We simultaneously characterize host and parasite gene expression profiles from 136 Malian children with symptomatic falciparum malaria and examine differences in the relative proportion of immune cells and parasite stages, as well as in gene expression, associated with infection and or patient characteristics. Parasitemia explains much of the variation in host and parasite gene expression, and infections with higher parasitemia display proportionally more neutrophils and fewer T cells, suggesting parasitemia-dependent neutrophil recruitment and/or T cell extravasation to secondary lymphoid organs. The child’s age also strongly correlates with variations in gene expression: Plasmodium falciparum genes associated with age suggest that older children carry more male gametocytes, while variations in host gene expression indicate a stronger innate response in younger children and stronger adaptive response in older children. These analyses highlight the variability in host responses and parasite regulation during P. falciparum symptomatic infections and emphasize the importance of considering the children’s age when studying and treating malaria infections.https://doi.org/10.1038/s41467-024-46416-3 |
| spellingShingle | Kieran Tebben Salif Yirampo Drissa Coulibaly Abdoulaye K. Koné Matthew B. Laurens Emily M. Stucke Ahmadou Dembélé Youssouf Tolo Karim Traoré Amadou Niangaly Andrea A. Berry Bourema Kouriba Christopher V. Plowe Ogobara K. Doumbo Kirsten E. Lyke Shannon Takala-Harrison Mahamadou A. Thera Mark A. Travassos David Serre Gene expression analyses reveal differences in children’s response to malaria according to their age Nature Communications |
| title | Gene expression analyses reveal differences in children’s response to malaria according to their age |
| title_full | Gene expression analyses reveal differences in children’s response to malaria according to their age |
| title_fullStr | Gene expression analyses reveal differences in children’s response to malaria according to their age |
| title_full_unstemmed | Gene expression analyses reveal differences in children’s response to malaria according to their age |
| title_short | Gene expression analyses reveal differences in children’s response to malaria according to their age |
| title_sort | gene expression analyses reveal differences in children s response to malaria according to their age |
| url | https://doi.org/10.1038/s41467-024-46416-3 |
| work_keys_str_mv | AT kierantebben geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT salifyirampo geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT drissacoulibaly geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT abdoulayekkone geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT matthewblaurens geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT emilymstucke geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT ahmadoudembele geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT youssouftolo geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT karimtraore geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT amadouniangaly geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT andreaaberry geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT bouremakouriba geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT christophervplowe geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT ogobarakdoumbo geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT kirstenelyke geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT shannontakalaharrison geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT mahamadouathera geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT markatravassos geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage AT davidserre geneexpressionanalysesrevealdifferencesinchildrensresponsetomalariaaccordingtotheirage |