BIOLOGICAL CONTROL OF SOIL -BORNE FUNGAL PATHOGENS
Seventeen bacterial isolates have been successfully isolated and purified from soil rhizosphere samples collected from different agricultural areas Buhayra, Sharqia and Alqilyubia of Egypt, and screened for production of chitinase enzymes had the highest chitinolytic activities amongst those investi...
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2019-03-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_43694_5b7c3892b04c4d12f94eaa6cb8f2ee3f.pdf |
| _version_ | 1827323578637352960 |
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| author | Magdy Madkour M. Afifi E. Metry I. Ismail |
| author_facet | Magdy Madkour M. Afifi E. Metry I. Ismail |
| author_sort | Magdy Madkour |
| collection | DOAJ |
| description | Seventeen bacterial isolates have been successfully isolated and purified from soil rhizosphere samples collected from different agricultural areas Buhayra, Sharqia and Alqilyubia of Egypt, and screened for production of chitinase enzymes had the highest chitinolytic activities amongst those investigated. Isolate S3-C and S1-C were Identified by biochemical, physiological tests and 16s rRNA gene primer as Bacillus cereus S3C, Gene Bank NCBI accession MK185696 and Bacillus cereus S1C accession MK185697. The production of chitinase by B.cereus S3C and Bacillus cereus S1C was optimized using colloidal chitin medium amended with 1% colloidal chitin at 30°C after five days of incubation. B.cereus S3C had potential for cell wall lysis of many phytopathogenic fungi tested such as Fusarium oxysporum and Rhizoctonia solani Potato phytopathogenic fungi by in vitro antagonistic test. The addition of B. cereus S3C chitinase was more effective than that of B.cereus S1C in increasing the resistance of Potato plants infected with various Soil- borne phytopathogenic fungi. |
| first_indexed | 2024-04-25T01:54:02Z |
| format | Article |
| id | doaj.art-448b5a6a21e44a01b72f3c3023f7ce4f |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:54:02Z |
| publishDate | 2019-03-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-448b5a6a21e44a01b72f3c3023f7ce4f2024-03-07T17:37:42ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852019-03-0127174976010.21608/ajs.2019.4369443694BIOLOGICAL CONTROL OF SOIL -BORNE FUNGAL PATHOGENSMagdy Madkour0M. Afifi1E. Metry2I. Ismail3Arid Lands Agriculture Research Institute, faculty of Agriculture, Ain-Shams universityHorticulture Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68 Hadyek Shoubra11241, Cairo, EgyptAgriculture Genetic Engineering Research Inst., Agric. Res. Center, Giza, EgyptAgriculture Genetic Engineering Research Inst., Agric. Res. Center, Giza, EgyptSeventeen bacterial isolates have been successfully isolated and purified from soil rhizosphere samples collected from different agricultural areas Buhayra, Sharqia and Alqilyubia of Egypt, and screened for production of chitinase enzymes had the highest chitinolytic activities amongst those investigated. Isolate S3-C and S1-C were Identified by biochemical, physiological tests and 16s rRNA gene primer as Bacillus cereus S3C, Gene Bank NCBI accession MK185696 and Bacillus cereus S1C accession MK185697. The production of chitinase by B.cereus S3C and Bacillus cereus S1C was optimized using colloidal chitin medium amended with 1% colloidal chitin at 30°C after five days of incubation. B.cereus S3C had potential for cell wall lysis of many phytopathogenic fungi tested such as Fusarium oxysporum and Rhizoctonia solani Potato phytopathogenic fungi by in vitro antagonistic test. The addition of B. cereus S3C chitinase was more effective than that of B.cereus S1C in increasing the resistance of Potato plants infected with various Soil- borne phytopathogenic fungi.https://ajs.journals.ekb.eg/article_43694_5b7c3892b04c4d12f94eaa6cb8f2ee3f.pdfbiological controlsoil bornefungal pathogensbacillus cereuschitinasecharacterizationoptimizationantifungal activity |
| spellingShingle | Magdy Madkour M. Afifi E. Metry I. Ismail BIOLOGICAL CONTROL OF SOIL -BORNE FUNGAL PATHOGENS Arab Universities Journal of Agricultural Sciences biological control soil borne fungal pathogens bacillus cereus chitinase characterization optimization antifungal activity |
| title | BIOLOGICAL CONTROL OF SOIL -BORNE FUNGAL PATHOGENS |
| title_full | BIOLOGICAL CONTROL OF SOIL -BORNE FUNGAL PATHOGENS |
| title_fullStr | BIOLOGICAL CONTROL OF SOIL -BORNE FUNGAL PATHOGENS |
| title_full_unstemmed | BIOLOGICAL CONTROL OF SOIL -BORNE FUNGAL PATHOGENS |
| title_short | BIOLOGICAL CONTROL OF SOIL -BORNE FUNGAL PATHOGENS |
| title_sort | biological control of soil borne fungal pathogens |
| topic | biological control soil borne fungal pathogens bacillus cereus chitinase characterization optimization antifungal activity |
| url | https://ajs.journals.ekb.eg/article_43694_5b7c3892b04c4d12f94eaa6cb8f2ee3f.pdf |
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