Effects of Kersen leaves extract (Muntingia calabura L.) on SGOT and SGPT levels of soft drink induced mice
This study aimed to analyse the antioxidant activity, total phenols, and flavonoids of kersen leaves (Muntingia calabura L.) extract (KLE) and determine the hepatoprotective activity of KLE in soft drink induced mice. Soft drinks induction causes liver injuries which increases the serum glutamic ox...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2019-07-01
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| Series: | Jurnal Gizi dan Pangan |
| Subjects: | |
| Online Access: | https://jurnal.ipb.ac.id/index.php/jgizipangan/article/view/22576 |
| _version_ | 1797672550618103808 |
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| author | Muhammad Nuzul Azhim Ash Siddiq Sri Anna Marliyati Hadi Riyadi Wiwin Winarsih |
| author_facet | Muhammad Nuzul Azhim Ash Siddiq Sri Anna Marliyati Hadi Riyadi Wiwin Winarsih |
| author_sort | Muhammad Nuzul Azhim Ash Siddiq |
| collection | DOAJ |
| description |
This study aimed to analyse the antioxidant activity, total phenols, and flavonoids of kersen leaves (Muntingia calabura L.) extract (KLE) and determine the hepatoprotective activity of KLE in soft drink induced mice. Soft drinks induction causes liver injuries which increases the serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) levels. Mice were divided into five treatment groups (n=5) and were given Tween 80 (1%) (normal control), Tween 80 (1%) and 7 ml soft drinks (negative control), KLE (1000, 1500, and 2000 mg/kg), and 7 ml soft drinks per oral once daily for 4 weeks. Antioxidant activity was measured by 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenols and flavonoids were analyzed by Folin-ciocalteu colorimetric and aluminium chloride (AlCl3) method. The blood samples were collected for biochemical analysis. The result showed that the KLE antioxidant activity were 11.11+0.50 ppm. Total phenols and flavonoids detected in the KLE samples were 2.19+0.12 mgGAE/g and 2.43+0.24 mgQE/g respectively. Mice treated with KLE had a significantly lower (p<0.05) serum SGOT and SGPT levels than negative control group. In conclusion, KLE has a potential hepatoprotective activity that works in synergy with its antioxidant activity.
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| first_indexed | 2024-03-11T21:31:43Z |
| format | Article |
| id | doaj.art-448ec03a1059491e85e0f1d323fb3780 |
| institution | Directory Open Access Journal |
| issn | 1978-1059 2407-0920 |
| language | English |
| last_indexed | 2024-03-11T21:31:43Z |
| publishDate | 2019-07-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Jurnal Gizi dan Pangan |
| spelling | doaj.art-448ec03a1059491e85e0f1d323fb37802023-09-27T08:43:00ZengBogor Agricultural UniversityJurnal Gizi dan Pangan1978-10592407-09202019-07-0114210.25182/jgp.2019.14.2.69-76Effects of Kersen leaves extract (Muntingia calabura L.) on SGOT and SGPT levels of soft drink induced miceMuhammad Nuzul Azhim Ash Siddiq0Sri Anna Marliyati1Hadi Riyadi2Wiwin Winarsih3Department of Community Nutrition, Faculty of Human Ecology, IPB University, Bogor 16680, IndonesiaDepartment of Community Nutrition, Faculty of Human Ecology, IPB University, Bogor 16680, IndonesiaDepartment of Community Nutrition, Faculty of Human Ecology, IPB University, Bogor 16680, IndonesiaDepartment Veterinary Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia This study aimed to analyse the antioxidant activity, total phenols, and flavonoids of kersen leaves (Muntingia calabura L.) extract (KLE) and determine the hepatoprotective activity of KLE in soft drink induced mice. Soft drinks induction causes liver injuries which increases the serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) levels. Mice were divided into five treatment groups (n=5) and were given Tween 80 (1%) (normal control), Tween 80 (1%) and 7 ml soft drinks (negative control), KLE (1000, 1500, and 2000 mg/kg), and 7 ml soft drinks per oral once daily for 4 weeks. Antioxidant activity was measured by 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenols and flavonoids were analyzed by Folin-ciocalteu colorimetric and aluminium chloride (AlCl3) method. The blood samples were collected for biochemical analysis. The result showed that the KLE antioxidant activity were 11.11+0.50 ppm. Total phenols and flavonoids detected in the KLE samples were 2.19+0.12 mgGAE/g and 2.43+0.24 mgQE/g respectively. Mice treated with KLE had a significantly lower (p<0.05) serum SGOT and SGPT levels than negative control group. In conclusion, KLE has a potential hepatoprotective activity that works in synergy with its antioxidant activity. https://jurnal.ipb.ac.id/index.php/jgizipangan/article/view/22576antioxidant activityMuntingia calabura L.SGOTSGPTsoft drink |
| spellingShingle | Muhammad Nuzul Azhim Ash Siddiq Sri Anna Marliyati Hadi Riyadi Wiwin Winarsih Effects of Kersen leaves extract (Muntingia calabura L.) on SGOT and SGPT levels of soft drink induced mice Jurnal Gizi dan Pangan antioxidant activity Muntingia calabura L. SGOT SGPT soft drink |
| title | Effects of Kersen leaves extract (Muntingia calabura L.) on SGOT and SGPT levels of soft drink induced mice |
| title_full | Effects of Kersen leaves extract (Muntingia calabura L.) on SGOT and SGPT levels of soft drink induced mice |
| title_fullStr | Effects of Kersen leaves extract (Muntingia calabura L.) on SGOT and SGPT levels of soft drink induced mice |
| title_full_unstemmed | Effects of Kersen leaves extract (Muntingia calabura L.) on SGOT and SGPT levels of soft drink induced mice |
| title_short | Effects of Kersen leaves extract (Muntingia calabura L.) on SGOT and SGPT levels of soft drink induced mice |
| title_sort | effects of kersen leaves extract muntingia calabura l on sgot and sgpt levels of soft drink induced mice |
| topic | antioxidant activity Muntingia calabura L. SGOT SGPT soft drink |
| url | https://jurnal.ipb.ac.id/index.php/jgizipangan/article/view/22576 |
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