Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains
In previous studies, the relaxant effect of Tymus vulgaris has been demonstrated on guinea pig tracheal chains. Therefore, in the present study, the relaxant effects of n-hexane, dichloromethane, methanol and aqueous fractions of Tymus vulgaris on tracheal chains of guinea pigs were examined. The re...
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BMC
2012-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100009 |
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| author | Rana Keyhanmanesh Mohammad H Boskabady |
| author_facet | Rana Keyhanmanesh Mohammad H Boskabady |
| author_sort | Rana Keyhanmanesh |
| collection | DOAJ |
| description | In previous studies, the relaxant effect of Tymus vulgaris has been demonstrated on guinea pig tracheal chains. Therefore, in the present study, the relaxant effects of n-hexane, dichloromethane, methanol and aqueous fractions of Tymus vulgaris on tracheal chains of guinea pigs were examined. The relaxant effects of four cumulative concentrations of each fraction (0.4, 0.8, 1.2 and 1.6 g%) in comparison to saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mM) were examined for their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 ìÌ methacholine (group 2, n = 7 for each group). In group 1, all concentrations of the n-hexane fraction and theophylline and three last concentrations (0.8, 1.2 and 1.6 g%) of dichloromethane and two higher concentrations (1.2 and 1.6 g%) of methanol fractions showed significant relaxant effects compared to that of saline (p<0.05 to p<0.001). In group 2, all concentrations of theophylline, n-hexane and dichloromethane fractions and three concentrations (0.8, 1.2 and 1.6 g%) of methanol and two higher concentrations (1.2 and 1.6 g%) of aqueous fractions showed significant relaxant effects compared to that of saline (p<0.05 to p<0.001). In addition, with group 1, the relaxant effect of all concentrations of all fractions except the n-hexane fraction, were significantly less than those of theophylline (p<0.05 to p<0.001). The n-hexane fraction showed higher relaxant effect than theophylline. The relaxant effect of all concentrations of the n-hexane fraction and the three last concentrations (0.8, 1.2 and 1.6 g%) of dichloromethane and aqueous fractions were significantly greater in group 2 than in group 1 (p<0.05 to p<0.001). There were significant positive correlations between the relaxant effects and concentrations for theophylline and all fractions (except aqueous fraction in group 1) in both groups, but a negative correlation for the aqueous fraction in group 1 (p<0.05 to p<0.001). These results showed a potent relaxant effect for n-hexane and weaker relaxant effect for other fractions from Tymus vulgaris on tracheal chains of guinea pigs. |
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| spelling | doaj.art-44932480ed39420caf74e51223dbf7492022-12-21T23:34:35ZengBMCBiological Research0716-97600717-62872012-01-014516773Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chainsRana KeyhanmaneshMohammad H BoskabadyIn previous studies, the relaxant effect of Tymus vulgaris has been demonstrated on guinea pig tracheal chains. Therefore, in the present study, the relaxant effects of n-hexane, dichloromethane, methanol and aqueous fractions of Tymus vulgaris on tracheal chains of guinea pigs were examined. The relaxant effects of four cumulative concentrations of each fraction (0.4, 0.8, 1.2 and 1.6 g%) in comparison to saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mM) were examined for their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 ìÌ methacholine (group 2, n = 7 for each group). In group 1, all concentrations of the n-hexane fraction and theophylline and three last concentrations (0.8, 1.2 and 1.6 g%) of dichloromethane and two higher concentrations (1.2 and 1.6 g%) of methanol fractions showed significant relaxant effects compared to that of saline (p<0.05 to p<0.001). In group 2, all concentrations of theophylline, n-hexane and dichloromethane fractions and three concentrations (0.8, 1.2 and 1.6 g%) of methanol and two higher concentrations (1.2 and 1.6 g%) of aqueous fractions showed significant relaxant effects compared to that of saline (p<0.05 to p<0.001). In addition, with group 1, the relaxant effect of all concentrations of all fractions except the n-hexane fraction, were significantly less than those of theophylline (p<0.05 to p<0.001). The n-hexane fraction showed higher relaxant effect than theophylline. The relaxant effect of all concentrations of the n-hexane fraction and the three last concentrations (0.8, 1.2 and 1.6 g%) of dichloromethane and aqueous fractions were significantly greater in group 2 than in group 1 (p<0.05 to p<0.001). There were significant positive correlations between the relaxant effects and concentrations for theophylline and all fractions (except aqueous fraction in group 1) in both groups, but a negative correlation for the aqueous fraction in group 1 (p<0.05 to p<0.001). These results showed a potent relaxant effect for n-hexane and weaker relaxant effect for other fractions from Tymus vulgaris on tracheal chains of guinea pigs.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100009Tymus vulgarisFractionsRelaxant effectsGuinea pigTrachea |
| spellingShingle | Rana Keyhanmanesh Mohammad H Boskabady Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains Biological Research Tymus vulgaris Fractions Relaxant effects Guinea pig Trachea |
| title | Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains |
| title_full | Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains |
| title_fullStr | Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains |
| title_full_unstemmed | Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains |
| title_short | Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains |
| title_sort | relaxant effects of different fractions from tymus vulgaris on guinea pig tracheal chains |
| topic | Tymus vulgaris Fractions Relaxant effects Guinea pig Trachea |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100009 |
| work_keys_str_mv | AT ranakeyhanmanesh relaxanteffectsofdifferentfractionsfromtymusvulgarisonguineapigtrachealchains AT mohammadhboskabady relaxanteffectsofdifferentfractionsfromtymusvulgarisonguineapigtrachealchains |