Desmosomal connectomics of all somatic muscles in an annelid larva
Cells form networks in animal tissues through synaptic, chemical, and adhesive links. Invertebrate muscle cells often connect to other cells through desmosomes, adhesive junctions anchored by intermediate filaments. To study desmosomal networks, we skeletonised 853 muscle cells and their desmosomal...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-12-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/71231 |
| _version_ | 1828054316346245120 |
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| author | Sanja Jasek Csaba Verasztó Emelie Brodrick Réza Shahidi Tom Kazimiers Alexandra Kerbl Gáspár Jékely |
| author_facet | Sanja Jasek Csaba Verasztó Emelie Brodrick Réza Shahidi Tom Kazimiers Alexandra Kerbl Gáspár Jékely |
| author_sort | Sanja Jasek |
| collection | DOAJ |
| description | Cells form networks in animal tissues through synaptic, chemical, and adhesive links. Invertebrate muscle cells often connect to other cells through desmosomes, adhesive junctions anchored by intermediate filaments. To study desmosomal networks, we skeletonised 853 muscle cells and their desmosomal partners in volume electron microscopy data covering an entire larva of the annelid Platynereis. Muscle cells adhere to each other, to epithelial, glial, ciliated, and bristle-producing cells and to the basal lamina, forming a desmosomal connectome of over 2000 cells. The aciculae – chitin rods that form an endoskeleton in the segmental appendages – are highly connected hubs in this network. This agrees with the many degrees of freedom of their movement, as revealed by video microscopy. Mapping motoneuron synapses to the desmosomal connectome allowed us to infer the extent of tissue influenced by motoneurons. Our work shows how cellular-level maps of synaptic and adherent force networks can elucidate body mechanics. |
| first_indexed | 2024-04-10T20:20:52Z |
| format | Article |
| id | doaj.art-449b675b509d417cbf1944992b8881aa |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-10T20:20:52Z |
| publishDate | 2022-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-449b675b509d417cbf1944992b8881aa2023-01-25T17:46:17ZengeLife Sciences Publications LtdeLife2050-084X2022-12-011110.7554/eLife.71231Desmosomal connectomics of all somatic muscles in an annelid larvaSanja Jasek0Csaba Verasztó1Emelie Brodrick2Réza Shahidi3Tom Kazimiers4Alexandra Kerbl5Gáspár Jékely6https://orcid.org/0000-0001-8496-9836Living Systems Institute, University of Exeter, Exeter, United KingdomLiving Systems Institute, University of Exeter, Exeter, United KingdomLiving Systems Institute, University of Exeter, Exeter, United KingdomLiving Systems Institute, University of Exeter, Exeter, United KingdomJanelia Research Campus, Ashburn, United States; kazmos GmbH, Dresden, GermanyLiving Systems Institute, University of Exeter, Exeter, United KingdomLiving Systems Institute, University of Exeter, Exeter, United KingdomCells form networks in animal tissues through synaptic, chemical, and adhesive links. Invertebrate muscle cells often connect to other cells through desmosomes, adhesive junctions anchored by intermediate filaments. To study desmosomal networks, we skeletonised 853 muscle cells and their desmosomal partners in volume electron microscopy data covering an entire larva of the annelid Platynereis. Muscle cells adhere to each other, to epithelial, glial, ciliated, and bristle-producing cells and to the basal lamina, forming a desmosomal connectome of over 2000 cells. The aciculae – chitin rods that form an endoskeleton in the segmental appendages – are highly connected hubs in this network. This agrees with the many degrees of freedom of their movement, as revealed by video microscopy. Mapping motoneuron synapses to the desmosomal connectome allowed us to infer the extent of tissue influenced by motoneurons. Our work shows how cellular-level maps of synaptic and adherent force networks can elucidate body mechanics.https://elifesciences.org/articles/71231desmosomemuscleconnectomicsbiological networkslocomotionannelid |
| spellingShingle | Sanja Jasek Csaba Verasztó Emelie Brodrick Réza Shahidi Tom Kazimiers Alexandra Kerbl Gáspár Jékely Desmosomal connectomics of all somatic muscles in an annelid larva eLife desmosome muscle connectomics biological networks locomotion annelid |
| title | Desmosomal connectomics of all somatic muscles in an annelid larva |
| title_full | Desmosomal connectomics of all somatic muscles in an annelid larva |
| title_fullStr | Desmosomal connectomics of all somatic muscles in an annelid larva |
| title_full_unstemmed | Desmosomal connectomics of all somatic muscles in an annelid larva |
| title_short | Desmosomal connectomics of all somatic muscles in an annelid larva |
| title_sort | desmosomal connectomics of all somatic muscles in an annelid larva |
| topic | desmosome muscle connectomics biological networks locomotion annelid |
| url | https://elifesciences.org/articles/71231 |
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