Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae.
Mycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Characteristic of this infection is the formation of grains. However, the processes leading to grain formation are not known. In this study, we employed a proteomic approa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-04-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0008190 |
| _version_ | 1818581562824851456 |
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| author | Gerard Sheehan Mickey Konings Wilson Lim Ahmed Fahal Kevin Kavanagh Wendy W J van de Sande |
| author_facet | Gerard Sheehan Mickey Konings Wilson Lim Ahmed Fahal Kevin Kavanagh Wendy W J van de Sande |
| author_sort | Gerard Sheehan |
| collection | DOAJ |
| description | Mycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Characteristic of this infection is the formation of grains. However, the processes leading to grain formation are not known. In this study, we employed a proteomic approach to characterise M. mycetomatis grain formation in Galleria mellonella larvae and map the processes leading to grain formation over time. For this, at 1 day, 3 days and 7 days post-inoculation, proteins from grains and hemolymph were extracted and analysed by label-free mass spectrometry. A total of 87, 51 and 48 M. mycetomatis proteins and 713, 997, 18 G. mellonella proteins were found in grains on day 1, 3 and 7 post-inoculation respectively. M. mycetomatis proteins were mainly involved in cellular metabolic processes and numerous enzymes were encountered. G. mellonella proteins were primarily involved in the nodulation process. The proteins identified were linked to nodulation and grain formation and four steps of grain formation were identified. The results of this proteomic approach could in the future be used to design novel strategies to interfere with mycetoma grain formation and to combat this difficult to treat infection. |
| first_indexed | 2024-12-16T07:35:28Z |
| format | Article |
| id | doaj.art-449e255320ce4cda84c8ce82bbc665c1 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-16T07:35:28Z |
| publishDate | 2020-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-449e255320ce4cda84c8ce82bbc665c12022-12-21T22:39:14ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352020-04-01144e000819010.1371/journal.pntd.0008190Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae.Gerard SheehanMickey KoningsWilson LimAhmed FahalKevin KavanaghWendy W J van de SandeMycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Characteristic of this infection is the formation of grains. However, the processes leading to grain formation are not known. In this study, we employed a proteomic approach to characterise M. mycetomatis grain formation in Galleria mellonella larvae and map the processes leading to grain formation over time. For this, at 1 day, 3 days and 7 days post-inoculation, proteins from grains and hemolymph were extracted and analysed by label-free mass spectrometry. A total of 87, 51 and 48 M. mycetomatis proteins and 713, 997, 18 G. mellonella proteins were found in grains on day 1, 3 and 7 post-inoculation respectively. M. mycetomatis proteins were mainly involved in cellular metabolic processes and numerous enzymes were encountered. G. mellonella proteins were primarily involved in the nodulation process. The proteins identified were linked to nodulation and grain formation and four steps of grain formation were identified. The results of this proteomic approach could in the future be used to design novel strategies to interfere with mycetoma grain formation and to combat this difficult to treat infection.https://doi.org/10.1371/journal.pntd.0008190 |
| spellingShingle | Gerard Sheehan Mickey Konings Wilson Lim Ahmed Fahal Kevin Kavanagh Wendy W J van de Sande Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. PLoS Neglected Tropical Diseases |
| title | Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
| title_full | Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
| title_fullStr | Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
| title_full_unstemmed | Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
| title_short | Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
| title_sort | proteomic analysis of the processes leading to madurella mycetomatis grain formation in galleria mellonella larvae |
| url | https://doi.org/10.1371/journal.pntd.0008190 |
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