p-S6 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma
Scarce information exists on the role of mTOR pathway proteins and their association to aggressiveness and prognosis of patients with canine oral cancers. We aimed to investigate the activated form of mTOR and its downstream S6 protein in canine oral squamous cell carcinoma (OSCC), and to evaluate p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/7/935 |
| _version_ | 1797407294216994816 |
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| author | Leonor Delgado Paula Brilhante-Simões Fernanda Garcez Luís Monteiro Isabel Pires Justina Prada |
| author_facet | Leonor Delgado Paula Brilhante-Simões Fernanda Garcez Luís Monteiro Isabel Pires Justina Prada |
| author_sort | Leonor Delgado |
| collection | DOAJ |
| description | Scarce information exists on the role of mTOR pathway proteins and their association to aggressiveness and prognosis of patients with canine oral cancers. We aimed to investigate the activated form of mTOR and its downstream S6 protein in canine oral squamous cell carcinoma (OSCC), and to evaluate potential associations between protein expression and clinic-pathologic variables and survival. For that we analysed p-mTOR and p-S6 protein expression by immunohistochemistry in 61 canine OSCCs. Multivariate analysis was conducted to examine their role in patients’ cancer-specific survival (CSS). p-mTOR and p-S6 expression were present in almost all cases. High-expression of p-mTOR was observed in 44 (72.1%) cases using extent score and 52 (85.2%) cases using intensity score. For p-S6, high expression was observed in 53 (86.9%) cases using extent score and in 54 (88.5%) cases using intensity score. An independent prognostic value for p-S6 extension (<i>p</i> = 0.027), tumour stage (<i>p</i> = 0.013) and treatment (<i>p</i> = 0.0009) was found in patients’ CSS analysis. Our data suggest that p-mTOR and p-S6 proteins are commonly expressed in canine OSCC and p-S6 expression is correlated with poor CSS in dogs with OSCC. More studies should be performed to identify possible therapeutic targets related with mTOR pathway for these patients. |
| first_indexed | 2024-03-09T03:39:15Z |
| format | Article |
| id | doaj.art-449f1fedeb214a4aab69f4bd934511b1 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T03:39:15Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-449f1fedeb214a4aab69f4bd934511b12023-12-03T14:43:27ZengMDPI AGBiomolecules2218-273X2022-07-0112793510.3390/biom12070935p-S6 as a Prognostic Biomarker in Canine Oral Squamous Cell CarcinomaLeonor Delgado0Paula Brilhante-Simões1Fernanda Garcez2Luís Monteiro3Isabel Pires4Justina Prada5Pathology Department, INNO Serviços Especializados em Veterinária, 4710-503 Braga, PortugalPathology Department, INNO Serviços Especializados em Veterinária, 4710-503 Braga, PortugalUNIPRO, Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences (IUCS), CESPU, 4585-116 Gandra, PortugalUNIPRO, Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences (IUCS), CESPU, 4585-116 Gandra, PortugalDepartment of Veterinary Science, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, PortugalDepartment of Veterinary Science, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, PortugalScarce information exists on the role of mTOR pathway proteins and their association to aggressiveness and prognosis of patients with canine oral cancers. We aimed to investigate the activated form of mTOR and its downstream S6 protein in canine oral squamous cell carcinoma (OSCC), and to evaluate potential associations between protein expression and clinic-pathologic variables and survival. For that we analysed p-mTOR and p-S6 protein expression by immunohistochemistry in 61 canine OSCCs. Multivariate analysis was conducted to examine their role in patients’ cancer-specific survival (CSS). p-mTOR and p-S6 expression were present in almost all cases. High-expression of p-mTOR was observed in 44 (72.1%) cases using extent score and 52 (85.2%) cases using intensity score. For p-S6, high expression was observed in 53 (86.9%) cases using extent score and in 54 (88.5%) cases using intensity score. An independent prognostic value for p-S6 extension (<i>p</i> = 0.027), tumour stage (<i>p</i> = 0.013) and treatment (<i>p</i> = 0.0009) was found in patients’ CSS analysis. Our data suggest that p-mTOR and p-S6 proteins are commonly expressed in canine OSCC and p-S6 expression is correlated with poor CSS in dogs with OSCC. More studies should be performed to identify possible therapeutic targets related with mTOR pathway for these patients.https://www.mdpi.com/2218-273X/12/7/935oral cancerp-mTOR and p-S6 expressionprognostic markerssurvival |
| spellingShingle | Leonor Delgado Paula Brilhante-Simões Fernanda Garcez Luís Monteiro Isabel Pires Justina Prada p-S6 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma Biomolecules oral cancer p-mTOR and p-S6 expression prognostic markers survival |
| title | p-S6 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_full | p-S6 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_fullStr | p-S6 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_full_unstemmed | p-S6 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_short | p-S6 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma |
| title_sort | p s6 as a prognostic biomarker in canine oral squamous cell carcinoma |
| topic | oral cancer p-mTOR and p-S6 expression prognostic markers survival |
| url | https://www.mdpi.com/2218-273X/12/7/935 |
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