In Vitro Study of Interactions of Carboxamide Derivatives of Amino Acid with BSA: Ultrasonic Interferometer
In this paper we account the interaction of the Carboxamide derivatives of amino acid viz 2-{[2-(cyclohexycarbamoyl) benzoyl] amino} propanoic acid (2CMPA), 2-Benzamido acetic acid 2-cyclohexyl carboxamide (2BA2C), 2-{[2-(cyclohexylcarbamoyl) benzoyl] amino}-3-methylbutanoic acid (2CA3MBA), 2-benzam...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Mato Grosso do Sul
2018-06-01
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| Series: | Orbital: The Electronic Journal of Chemistry |
| Subjects: | |
| Online Access: | https://periodicos.ufms.br/index.php/orbital/article/view/16059 |
| _version_ | 1797946834000281600 |
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| author | Shrikant Bandupant Thakare Pradip Vitthalrao Tekade Ajay Madhukarrao Pisuddes |
| author_facet | Shrikant Bandupant Thakare Pradip Vitthalrao Tekade Ajay Madhukarrao Pisuddes |
| author_sort | Shrikant Bandupant Thakare |
| collection | DOAJ |
| description | In this paper we account the interaction of the Carboxamide derivatives of amino acid viz 2-{[2-(cyclohexycarbamoyl) benzoyl] amino} propanoic acid (2CMPA), 2-Benzamido acetic acid 2-cyclohexyl carboxamide (2BA2C), 2-{[2-(cyclohexylcarbamoyl) benzoyl] amino}-3-methylbutanoic acid (2CA3MBA), 2-benzamido-4-methylpentanoic acid-2-cyclohexyl carboxamide (2-BMCA) and 2-{[2-(cyclohexycarbamoyl) benzoyl] amino}-4-(methylsulfanyl) butanoic acid (2CA4MBA) with protein Bovine serum albumin (BSA) using ultrasonic interferometer technique. Ultrasonic velocity for complex solution of different compounds of carboxamide derivatives of amino acid with BSA has been measured at their different composition using ultrasonic interferometer. Difference in the ultrasonic velocity at different compositions of complex is measure of binding of the compounds with BSA. Binding effect at various pH viz. 3, 4 and 5 shows that compounds bound to the BSA more significantly at acidic pH and association constant decreases with increase in pH value. Scatchard analysis gives the values of association constants (Kf) for all the compounds at pH 3, 4 and 5 respectively.
DOI: http://dx.doi.org/10.17807/orbital.v10i5.1161
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| first_indexed | 2024-04-10T21:17:18Z |
| format | Article |
| id | doaj.art-44a5e0a445ee40ad859bdd159c900207 |
| institution | Directory Open Access Journal |
| issn | 1984-6428 |
| language | English |
| last_indexed | 2024-04-10T21:17:18Z |
| publishDate | 2018-06-01 |
| publisher | Universidade Federal de Mato Grosso do Sul |
| record_format | Article |
| series | Orbital: The Electronic Journal of Chemistry |
| spelling | doaj.art-44a5e0a445ee40ad859bdd159c9002072023-01-20T10:54:22ZengUniversidade Federal de Mato Grosso do SulOrbital: The Electronic Journal of Chemistry1984-64282018-06-01105In Vitro Study of Interactions of Carboxamide Derivatives of Amino Acid with BSA: Ultrasonic InterferometerShrikant Bandupant Thakare0Pradip Vitthalrao Tekade1Ajay Madhukarrao Pisuddes2Departement of Chemistry, Jankidevi Bajaj College of Science WardhaDepartement of Chemistry, Jankidevi Bajaj College of Science WardhaDepartement of Chemistry, Jankidevi Bajaj College of Science WardhaIn this paper we account the interaction of the Carboxamide derivatives of amino acid viz 2-{[2-(cyclohexycarbamoyl) benzoyl] amino} propanoic acid (2CMPA), 2-Benzamido acetic acid 2-cyclohexyl carboxamide (2BA2C), 2-{[2-(cyclohexylcarbamoyl) benzoyl] amino}-3-methylbutanoic acid (2CA3MBA), 2-benzamido-4-methylpentanoic acid-2-cyclohexyl carboxamide (2-BMCA) and 2-{[2-(cyclohexycarbamoyl) benzoyl] amino}-4-(methylsulfanyl) butanoic acid (2CA4MBA) with protein Bovine serum albumin (BSA) using ultrasonic interferometer technique. Ultrasonic velocity for complex solution of different compounds of carboxamide derivatives of amino acid with BSA has been measured at their different composition using ultrasonic interferometer. Difference in the ultrasonic velocity at different compositions of complex is measure of binding of the compounds with BSA. Binding effect at various pH viz. 3, 4 and 5 shows that compounds bound to the BSA more significantly at acidic pH and association constant decreases with increase in pH value. Scatchard analysis gives the values of association constants (Kf) for all the compounds at pH 3, 4 and 5 respectively. DOI: http://dx.doi.org/10.17807/orbital.v10i5.1161 https://periodicos.ufms.br/index.php/orbital/article/view/16059ultrasonic interferometerbovine serum albumincarboxamideassociation constantscatchard analysis |
| spellingShingle | Shrikant Bandupant Thakare Pradip Vitthalrao Tekade Ajay Madhukarrao Pisuddes In Vitro Study of Interactions of Carboxamide Derivatives of Amino Acid with BSA: Ultrasonic Interferometer Orbital: The Electronic Journal of Chemistry ultrasonic interferometer bovine serum albumin carboxamide association constant scatchard analysis |
| title | In Vitro Study of Interactions of Carboxamide Derivatives of Amino Acid with BSA: Ultrasonic Interferometer |
| title_full | In Vitro Study of Interactions of Carboxamide Derivatives of Amino Acid with BSA: Ultrasonic Interferometer |
| title_fullStr | In Vitro Study of Interactions of Carboxamide Derivatives of Amino Acid with BSA: Ultrasonic Interferometer |
| title_full_unstemmed | In Vitro Study of Interactions of Carboxamide Derivatives of Amino Acid with BSA: Ultrasonic Interferometer |
| title_short | In Vitro Study of Interactions of Carboxamide Derivatives of Amino Acid with BSA: Ultrasonic Interferometer |
| title_sort | in vitro study of interactions of carboxamide derivatives of amino acid with bsa ultrasonic interferometer |
| topic | ultrasonic interferometer bovine serum albumin carboxamide association constant scatchard analysis |
| url | https://periodicos.ufms.br/index.php/orbital/article/view/16059 |
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