The effects of Bifidobacterium Bifidum (BBCWF) on proliferation of K562 cell line
Background & Objective: In the previous studies, the beneficial effects of oral administration of probiotic bacteria in treatment of some gastrointestinal cancer have been documented. The main aim of this study was to evaluate the direct effects of the bacterial cell wall extract Bifidobacterium...
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| Format: | Article |
| Language: | fas |
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Fasa University of Medical Sciences
2017-06-01
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| Series: | Journal of Fasa University of Medical Sciences |
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| Online Access: | http://journal.fums.ac.ir/browse.php?a_code=A-10-1115-2&slc_lang=en&sid=1 |
| _version_ | 1819084734851973120 |
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| author | Leila zarei mysam abtahi foroshani Atefeh Garajedagi Hadi Esmaeili govarchin galeh |
| author_facet | Leila zarei mysam abtahi foroshani Atefeh Garajedagi Hadi Esmaeili govarchin galeh |
| author_sort | Leila zarei |
| collection | DOAJ |
| description | Background & Objective: In the previous studies, the beneficial effects of oral administration of probiotic bacteria in treatment of some gastrointestinal cancer have been documented. The main aim of this study was to evaluate the direct effects of the bacterial cell wall extract Bifidobacterium bifidum on proliferation of human erythromyeloid cancer cell line K562.
Material & methods: After culturing the bacteria, and preparing cell wall fractions, dilutions of 1000, 2000, 4000 and 8000 µg/ml bacteria cell walls were prepared in sterile conditions. K562 cells and peripheral blood mononuclear cells (PBMC) were cultured in a conventional manner in the presence of different concentrations of bacteria from cell wall extract with the doxorubicin concentration of 0.1 mM. In order to assess the anticancer property of the abovementioned extract the MTT test was utilized. The T-Student test was used in order to analyze the data using SPSS 21.
Results: The minimum effective concentration of bacteria extract on the viability of K562 cells and PBMC was 2000 and 4000 µg/ml, respectively.
Conclusion: The selected bacterial extract killed more cancer cells than PBMC cells. |
| first_indexed | 2024-12-21T20:53:10Z |
| format | Article |
| id | doaj.art-44a667ef535e44b19ff940bcc789314d |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2228-7329 |
| language | fas |
| last_indexed | 2024-12-21T20:53:10Z |
| publishDate | 2017-06-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasa University of Medical Sciences |
| spelling | doaj.art-44a667ef535e44b19ff940bcc789314d2022-12-21T18:50:39ZfasFasa University of Medical SciencesJournal of Fasa University of Medical Sciences2228-51052228-73292017-06-01712127The effects of Bifidobacterium Bifidum (BBCWF) on proliferation of K562 cell lineLeila zarei0mysam abtahi foroshani1Atefeh Garajedagi2Hadi Esmaeili govarchin galeh3 Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran Department of Microbiology, Faculty of Veterinary Sciences, Urmia University, Urmia, Iran Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran Department of Microbiology, Faculty of Veterinary Sciences, Urmia University, Urmia, Iran Background & Objective: In the previous studies, the beneficial effects of oral administration of probiotic bacteria in treatment of some gastrointestinal cancer have been documented. The main aim of this study was to evaluate the direct effects of the bacterial cell wall extract Bifidobacterium bifidum on proliferation of human erythromyeloid cancer cell line K562. Material & methods: After culturing the bacteria, and preparing cell wall fractions, dilutions of 1000, 2000, 4000 and 8000 µg/ml bacteria cell walls were prepared in sterile conditions. K562 cells and peripheral blood mononuclear cells (PBMC) were cultured in a conventional manner in the presence of different concentrations of bacteria from cell wall extract with the doxorubicin concentration of 0.1 mM. In order to assess the anticancer property of the abovementioned extract the MTT test was utilized. The T-Student test was used in order to analyze the data using SPSS 21. Results: The minimum effective concentration of bacteria extract on the viability of K562 cells and PBMC was 2000 and 4000 µg/ml, respectively. Conclusion: The selected bacterial extract killed more cancer cells than PBMC cells.http://journal.fums.ac.ir/browse.php?a_code=A-10-1115-2&slc_lang=en&sid=1Keywords: K562 Bifidobacterium Bifidum cell wall fraction peripheral blood mononuclear cell |
| spellingShingle | Leila zarei mysam abtahi foroshani Atefeh Garajedagi Hadi Esmaeili govarchin galeh The effects of Bifidobacterium Bifidum (BBCWF) on proliferation of K562 cell line Journal of Fasa University of Medical Sciences Keywords: K562 Bifidobacterium Bifidum cell wall fraction peripheral blood mononuclear cell |
| title | The effects of Bifidobacterium Bifidum (BBCWF) on proliferation of K562 cell line |
| title_full | The effects of Bifidobacterium Bifidum (BBCWF) on proliferation of K562 cell line |
| title_fullStr | The effects of Bifidobacterium Bifidum (BBCWF) on proliferation of K562 cell line |
| title_full_unstemmed | The effects of Bifidobacterium Bifidum (BBCWF) on proliferation of K562 cell line |
| title_short | The effects of Bifidobacterium Bifidum (BBCWF) on proliferation of K562 cell line |
| title_sort | effects of bifidobacterium bifidum bbcwf on proliferation of k562 cell line |
| topic | Keywords: K562 Bifidobacterium Bifidum cell wall fraction peripheral blood mononuclear cell |
| url | http://journal.fums.ac.ir/browse.php?a_code=A-10-1115-2&slc_lang=en&sid=1 |
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