Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated Environment
There is a growing interest in plant microbiome’s engineering to optimize desired functions such as improved phytoremediation. This study is aimed at examining the microbial communities inhabiting the roots and rhizospheres of two <i>Salix miyabeana</i> cultivars that had been grown in a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/2/145 |
| _version_ | 1797478894515781632 |
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| author | Maxime Fortin Faubert Michel Labrecque Mohamed Hijri |
| author_facet | Maxime Fortin Faubert Michel Labrecque Mohamed Hijri |
| author_sort | Maxime Fortin Faubert |
| collection | DOAJ |
| description | There is a growing interest in plant microbiome’s engineering to optimize desired functions such as improved phytoremediation. This study is aimed at examining the microbial communities inhabiting the roots and rhizospheres of two <i>Salix miyabeana</i> cultivars that had been grown in a short-rotation intensive culture (SRIC) system for six years in a soil contaminated with the discharge from a petrochemical factory. DNA was extracted from roots and rhizospheric soils, and fungal ITS and bacterial and archaeal 16S rDNA regions were amplified and sequenced using Illumina MiSeq technology. Cultivars ‘SX61’ and ‘SX64’ were found to harbor a similar diversity of fungal, bacterial, and archaeal amplicon sequence variants (ASVs). As expected, a greater microbial diversity was found in the rhizosphere biotope than in the roots of both cultivars, except for cultivar ‘SX64’, where a similar fungal diversity was observed in both biotopes. However, we found that microbial community structures were cultivar- and biotope-specific. Although the implication of some identified taxa for plant adaptability and biomass production capacity remains to be explored, this study provides valuable and useful information regarding microbes that could potentially favor the implantation and phytoremediation efficiency of <i>Salix miyabeana</i> in mixed contamination sites in similar climatic environments. |
| first_indexed | 2024-03-09T21:38:06Z |
| format | Article |
| id | doaj.art-44a73b9add3a45ebaf9099bc0bf7274a |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T21:38:06Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-44a73b9add3a45ebaf9099bc0bf7274a2023-11-23T20:37:32ZengMDPI AGJournal of Fungi2309-608X2022-01-018214510.3390/jof8020145Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated EnvironmentMaxime Fortin Faubert0Michel Labrecque1Mohamed Hijri2Institut de Recherche en Biologie végétale, Département de Sciences Biologiques, Université de Montréal and Jardin Botanique de Montréal, 4101 Sherbrooke East, Montreal, QC H1X 2B2, CanadaInstitut de Recherche en Biologie végétale, Département de Sciences Biologiques, Université de Montréal and Jardin Botanique de Montréal, 4101 Sherbrooke East, Montreal, QC H1X 2B2, CanadaAfrican Genome Center, Mohammed VI Polytechnic University (UM6P), Lot 660, Hay Moulay Rachid, Ben Guerir 43150, MoroccoThere is a growing interest in plant microbiome’s engineering to optimize desired functions such as improved phytoremediation. This study is aimed at examining the microbial communities inhabiting the roots and rhizospheres of two <i>Salix miyabeana</i> cultivars that had been grown in a short-rotation intensive culture (SRIC) system for six years in a soil contaminated with the discharge from a petrochemical factory. DNA was extracted from roots and rhizospheric soils, and fungal ITS and bacterial and archaeal 16S rDNA regions were amplified and sequenced using Illumina MiSeq technology. Cultivars ‘SX61’ and ‘SX64’ were found to harbor a similar diversity of fungal, bacterial, and archaeal amplicon sequence variants (ASVs). As expected, a greater microbial diversity was found in the rhizosphere biotope than in the roots of both cultivars, except for cultivar ‘SX64’, where a similar fungal diversity was observed in both biotopes. However, we found that microbial community structures were cultivar- and biotope-specific. Although the implication of some identified taxa for plant adaptability and biomass production capacity remains to be explored, this study provides valuable and useful information regarding microbes that could potentially favor the implantation and phytoremediation efficiency of <i>Salix miyabeana</i> in mixed contamination sites in similar climatic environments.https://www.mdpi.com/2309-608X/8/2/145<i>Salix</i>phytoremediationshort-rotation intensive culture (SRIC)soil contaminantsfungibacteria |
| spellingShingle | Maxime Fortin Faubert Michel Labrecque Mohamed Hijri Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated Environment Journal of Fungi <i>Salix</i> phytoremediation short-rotation intensive culture (SRIC) soil contaminants fungi bacteria |
| title | Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated Environment |
| title_full | Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated Environment |
| title_fullStr | Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated Environment |
| title_full_unstemmed | Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated Environment |
| title_short | Ectomycorrhizal Fungi Dominated the Root and Rhizosphere Microbial Communities of Two Willow Cultivars Grown for Six-Years in a Mixed-Contaminated Environment |
| title_sort | ectomycorrhizal fungi dominated the root and rhizosphere microbial communities of two willow cultivars grown for six years in a mixed contaminated environment |
| topic | <i>Salix</i> phytoremediation short-rotation intensive culture (SRIC) soil contaminants fungi bacteria |
| url | https://www.mdpi.com/2309-608X/8/2/145 |
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