Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial
The biological variability of semen and sperm DNA fragmentation (SDF) parameters in a longitudinal intrauterine insemination (IUI) trial over multiple IUI cycles was investigated. A TUNEL assay was used for SDF testing, both before and after density gradient centrifugation. A significant age effect...
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MDPI AG
2022-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/11/1826 |
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| author | Usha Punjabi Ella Roelant Kris Peeters Ilse Goovaerts Helga Van Mulders Diane De Neubourg |
| author_facet | Usha Punjabi Ella Roelant Kris Peeters Ilse Goovaerts Helga Van Mulders Diane De Neubourg |
| author_sort | Usha Punjabi |
| collection | DOAJ |
| description | The biological variability of semen and sperm DNA fragmentation (SDF) parameters in a longitudinal intrauterine insemination (IUI) trial over multiple IUI cycles was investigated. A TUNEL assay was used for SDF testing, both before and after density gradient centrifugation. A significant age effect was observed: while semen parameters deteriorated with advancing age, on average, higher SDF values were observed for older males. There was quite some variability observed for both semen and SDF variables. Using fertile threshold values, three patient categories were distinguished: those with a high SDF in all samples, those with low SDF in all samples and those who fluctuated between high and low during the whole IUI trial. Density gradient centrifugation increases SDF. However, the three patient categories react differently after semen processing. A large percentage of those with high SDF retain their high SDF even after gradient centrifugation. The SDF fluctuaters react with a high SDF after gradient centrifugation. The low SDF category, on the contrary, distributes itself evenly between the three categories after gradient centrifugation. SDF testing after semen processing might be indispensable for therapeutic purposes, probably influencing medical decision-making. In order to isolate fluctuaters, a second SDF testing might be advocated in certain cases. SDF after semen processing is indispensable for therapeutic management. |
| first_indexed | 2024-03-09T18:54:40Z |
| format | Article |
| id | doaj.art-44a753b618904293adf6b7cd3676a5c4 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T18:54:40Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-44a753b618904293adf6b7cd3676a5c42023-11-24T05:31:34ZengMDPI AGLife2075-17292022-11-011211182610.3390/life12111826Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination TrialUsha Punjabi0Ella Roelant1Kris Peeters2Ilse Goovaerts3Helga Van Mulders4Diane De Neubourg5Centre for Reproductive Medicine, Antwerp University Hospital, 2650 Edegem, BelgiumClinical Trial Centre (CTC), CRC Antwerp, Antwerp University Hospital, University of Antwerp, Drie Eikenstraat 655, 2650 Edegem, BelgiumCentre for Reproductive Medicine, Antwerp University Hospital, 2650 Edegem, BelgiumCentre for Reproductive Medicine, Antwerp University Hospital, 2650 Edegem, BelgiumCentre for Reproductive Medicine, Antwerp University Hospital, 2650 Edegem, BelgiumCentre for Reproductive Medicine, Antwerp University Hospital, 2650 Edegem, BelgiumThe biological variability of semen and sperm DNA fragmentation (SDF) parameters in a longitudinal intrauterine insemination (IUI) trial over multiple IUI cycles was investigated. A TUNEL assay was used for SDF testing, both before and after density gradient centrifugation. A significant age effect was observed: while semen parameters deteriorated with advancing age, on average, higher SDF values were observed for older males. There was quite some variability observed for both semen and SDF variables. Using fertile threshold values, three patient categories were distinguished: those with a high SDF in all samples, those with low SDF in all samples and those who fluctuated between high and low during the whole IUI trial. Density gradient centrifugation increases SDF. However, the three patient categories react differently after semen processing. A large percentage of those with high SDF retain their high SDF even after gradient centrifugation. The SDF fluctuaters react with a high SDF after gradient centrifugation. The low SDF category, on the contrary, distributes itself evenly between the three categories after gradient centrifugation. SDF testing after semen processing might be indispensable for therapeutic purposes, probably influencing medical decision-making. In order to isolate fluctuaters, a second SDF testing might be advocated in certain cases. SDF after semen processing is indispensable for therapeutic management.https://www.mdpi.com/2075-1729/12/11/1826sperm DNA fragmentationintrauterine inseminationdensity gradient centrifugationmale infertility |
| spellingShingle | Usha Punjabi Ella Roelant Kris Peeters Ilse Goovaerts Helga Van Mulders Diane De Neubourg Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial Life sperm DNA fragmentation intrauterine insemination density gradient centrifugation male infertility |
| title | Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial |
| title_full | Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial |
| title_fullStr | Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial |
| title_full_unstemmed | Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial |
| title_short | Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial |
| title_sort | variability in sperm dna fragmentation in men with mild unexplained subfertility in a prospective longitudinal intrauterine insemination trial |
| topic | sperm DNA fragmentation intrauterine insemination density gradient centrifugation male infertility |
| url | https://www.mdpi.com/2075-1729/12/11/1826 |
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