| Summary: | Gentiana manshurica Kitag is a perennial medicinal plant with high economic value. In this study, we first reported the complete chloroplast (cp) genome of G. manshurica. The total length of the cp genome was 149,185 bp in length, consisting of a large single-copy (LSC) region of 81,347 bp, a small single-copy (SSC) region of 17,268 bp, and a pair of inverted repeats (IRs) regions of 25,285 bp each. The cp genome contained 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The overall GC content was 37.61%. The phylogenetic analysis indicated that the G. manshurica belongs to the tribe Gentianeae, and showed a close relationship with other Gentiana species.
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