Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix.
A range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6197626?pdf=render |
| _version_ | 1811206150913261568 |
|---|---|
| author | C Mendoza-Topaz G Nelson G Howard S Hafner P Rademacher M Frick B J Nichols |
| author_facet | C Mendoza-Topaz G Nelson G Howard S Hafner P Rademacher M Frick B J Nichols |
| author_sort | C Mendoza-Topaz |
| collection | DOAJ |
| description | A range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-free insight into how these cells respond to the absence of caveolae. Components of the extracellular matrix were decisively over-represented within the set of genes displaying altered expression in CAV1-/- MEFs when compared to congenic wild-type controls. This was confirmed biochemically and by imaging for selected examples. Up-regulation of components of the extracellular matrix was also observed in a second cell line, NIH-3T3 cells genome edited to delete CAV1. Up-regulation of components of the extracellular matrix was detected in vivo by assessing collagen deposition and compliance of CAV1-/- lungs. We discuss the implications of these findings in terms of the cellular function of caveolae. |
| first_indexed | 2024-04-12T03:43:03Z |
| format | Article |
| id | doaj.art-44a7a31a540b495fb8918556185614d8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T03:43:03Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-44a7a31a540b495fb8918556185614d82022-12-22T03:49:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011310e020530610.1371/journal.pone.0205306Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix.C Mendoza-TopazG NelsonG HowardS HafnerP RademacherM FrickB J NicholsA range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-free insight into how these cells respond to the absence of caveolae. Components of the extracellular matrix were decisively over-represented within the set of genes displaying altered expression in CAV1-/- MEFs when compared to congenic wild-type controls. This was confirmed biochemically and by imaging for selected examples. Up-regulation of components of the extracellular matrix was also observed in a second cell line, NIH-3T3 cells genome edited to delete CAV1. Up-regulation of components of the extracellular matrix was detected in vivo by assessing collagen deposition and compliance of CAV1-/- lungs. We discuss the implications of these findings in terms of the cellular function of caveolae.http://europepmc.org/articles/PMC6197626?pdf=render |
| spellingShingle | C Mendoza-Topaz G Nelson G Howard S Hafner P Rademacher M Frick B J Nichols Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. PLoS ONE |
| title | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
| title_full | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
| title_fullStr | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
| title_full_unstemmed | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
| title_short | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
| title_sort | cells respond to deletion of cav1 by increasing synthesis of extracellular matrix |
| url | http://europepmc.org/articles/PMC6197626?pdf=render |
| work_keys_str_mv | AT cmendozatopaz cellsrespondtodeletionofcav1byincreasingsynthesisofextracellularmatrix AT gnelson cellsrespondtodeletionofcav1byincreasingsynthesisofextracellularmatrix AT ghoward cellsrespondtodeletionofcav1byincreasingsynthesisofextracellularmatrix AT shafner cellsrespondtodeletionofcav1byincreasingsynthesisofextracellularmatrix AT prademacher cellsrespondtodeletionofcav1byincreasingsynthesisofextracellularmatrix AT mfrick cellsrespondtodeletionofcav1byincreasingsynthesisofextracellularmatrix AT bjnichols cellsrespondtodeletionofcav1byincreasingsynthesisofextracellularmatrix |