Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix.
A range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC6197626?pdf=render |
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author | C Mendoza-Topaz G Nelson G Howard S Hafner P Rademacher M Frick B J Nichols |
author_facet | C Mendoza-Topaz G Nelson G Howard S Hafner P Rademacher M Frick B J Nichols |
author_sort | C Mendoza-Topaz |
collection | DOAJ |
description | A range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-free insight into how these cells respond to the absence of caveolae. Components of the extracellular matrix were decisively over-represented within the set of genes displaying altered expression in CAV1-/- MEFs when compared to congenic wild-type controls. This was confirmed biochemically and by imaging for selected examples. Up-regulation of components of the extracellular matrix was also observed in a second cell line, NIH-3T3 cells genome edited to delete CAV1. Up-regulation of components of the extracellular matrix was detected in vivo by assessing collagen deposition and compliance of CAV1-/- lungs. We discuss the implications of these findings in terms of the cellular function of caveolae. |
first_indexed | 2024-04-12T03:43:03Z |
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institution | Directory Open Access Journal |
issn | 1932-6203 |
language | English |
last_indexed | 2024-04-12T03:43:03Z |
publishDate | 2018-01-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS ONE |
spelling | doaj.art-44a7a31a540b495fb8918556185614d82022-12-22T03:49:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011310e020530610.1371/journal.pone.0205306Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix.C Mendoza-TopazG NelsonG HowardS HafnerP RademacherM FrickB J NicholsA range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice (CAV1-/- MEFs), and thereby to gain hypothesis-free insight into how these cells respond to the absence of caveolae. Components of the extracellular matrix were decisively over-represented within the set of genes displaying altered expression in CAV1-/- MEFs when compared to congenic wild-type controls. This was confirmed biochemically and by imaging for selected examples. Up-regulation of components of the extracellular matrix was also observed in a second cell line, NIH-3T3 cells genome edited to delete CAV1. Up-regulation of components of the extracellular matrix was detected in vivo by assessing collagen deposition and compliance of CAV1-/- lungs. We discuss the implications of these findings in terms of the cellular function of caveolae.http://europepmc.org/articles/PMC6197626?pdf=render |
spellingShingle | C Mendoza-Topaz G Nelson G Howard S Hafner P Rademacher M Frick B J Nichols Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. PLoS ONE |
title | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
title_full | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
title_fullStr | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
title_full_unstemmed | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
title_short | Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. |
title_sort | cells respond to deletion of cav1 by increasing synthesis of extracellular matrix |
url | http://europepmc.org/articles/PMC6197626?pdf=render |
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