Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells
Campylobacter jejuni is the major bacterial cause of foodborne gastroenteritis worldwide. Mechanistically, how this pathogen interacts with intrinsic defence machinery of human intestinal epithelial cells (IECs) remains elusive. To address this, we investigated how C. jejuni counteracts the intracel...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi-Wiley
2023-01-01
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| Series: | Cellular Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2023/3286330 |
| _version_ | 1811165468626518016 |
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| author | Geunhye Hong Cadi Davies Zahra Omole Janie Liaw Anna D. Grabowska Barbara Canonico Nicolae Corcionivoschi Brendan W. Wren Nick Dorrell Abdi Elmi Ozan Gundogdu |
| author_facet | Geunhye Hong Cadi Davies Zahra Omole Janie Liaw Anna D. Grabowska Barbara Canonico Nicolae Corcionivoschi Brendan W. Wren Nick Dorrell Abdi Elmi Ozan Gundogdu |
| author_sort | Geunhye Hong |
| collection | DOAJ |
| description | Campylobacter jejuni is the major bacterial cause of foodborne gastroenteritis worldwide. Mechanistically, how this pathogen interacts with intrinsic defence machinery of human intestinal epithelial cells (IECs) remains elusive. To address this, we investigated how C. jejuni counteracts the intracellular and extracellular reactive oxygen species (ROS) in IECs. Our work shows that C. jejuni differentially regulates intracellular and extracellular ROS production in human T84 and Caco-2 cells. C. jejuni downregulates the transcription and translation of nicotinamide adenine dinucleotide phosphate (NAPDH) oxidase (NOX1), a key ROS-generating enzyme in IECs and antioxidant defence genes CAT and SOD1. Furthermore, inhibition of NOX1 by diphenylene iodonium (DPI) and siRNA reduced C. jejuni ability to interact, invade, and intracellularly survive within T84 and Caco-2 cells. Collectively, these findings provide mechanistic insight into how C. jejuni modulates the IEC defence machinery. |
| first_indexed | 2024-04-10T15:37:37Z |
| format | Article |
| id | doaj.art-44ad18a07c584fa8ba09f381bec1ebca |
| institution | Directory Open Access Journal |
| issn | 1462-5822 |
| language | English |
| last_indexed | 2024-04-10T15:37:37Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi-Wiley |
| record_format | Article |
| series | Cellular Microbiology |
| spelling | doaj.art-44ad18a07c584fa8ba09f381bec1ebca2023-02-13T01:08:47ZengHindawi-WileyCellular Microbiology1462-58222023-01-01202310.1155/2023/3286330Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial CellsGeunhye Hong0Cadi Davies1Zahra Omole2Janie Liaw3Anna D. Grabowska4Barbara Canonico5Nicolae Corcionivoschi6Brendan W. Wren7Nick Dorrell8Abdi Elmi9Ozan Gundogdu10Faculty of Infectious and Tropical DiseasesFaculty of Infectious and Tropical DiseasesFaculty of Infectious and Tropical DiseasesFaculty of Infectious and Tropical DiseasesDepartment of BiophysicsDepartment of Biomolecular SciencesBacteriology BranchFaculty of Infectious and Tropical DiseasesFaculty of Infectious and Tropical DiseasesFaculty of Infectious and Tropical DiseasesFaculty of Infectious and Tropical DiseasesCampylobacter jejuni is the major bacterial cause of foodborne gastroenteritis worldwide. Mechanistically, how this pathogen interacts with intrinsic defence machinery of human intestinal epithelial cells (IECs) remains elusive. To address this, we investigated how C. jejuni counteracts the intracellular and extracellular reactive oxygen species (ROS) in IECs. Our work shows that C. jejuni differentially regulates intracellular and extracellular ROS production in human T84 and Caco-2 cells. C. jejuni downregulates the transcription and translation of nicotinamide adenine dinucleotide phosphate (NAPDH) oxidase (NOX1), a key ROS-generating enzyme in IECs and antioxidant defence genes CAT and SOD1. Furthermore, inhibition of NOX1 by diphenylene iodonium (DPI) and siRNA reduced C. jejuni ability to interact, invade, and intracellularly survive within T84 and Caco-2 cells. Collectively, these findings provide mechanistic insight into how C. jejuni modulates the IEC defence machinery.http://dx.doi.org/10.1155/2023/3286330 |
| spellingShingle | Geunhye Hong Cadi Davies Zahra Omole Janie Liaw Anna D. Grabowska Barbara Canonico Nicolae Corcionivoschi Brendan W. Wren Nick Dorrell Abdi Elmi Ozan Gundogdu Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells Cellular Microbiology |
| title | Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells |
| title_full | Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells |
| title_fullStr | Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells |
| title_full_unstemmed | Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells |
| title_short | Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells |
| title_sort | campylobacter jejuni modulates reactive oxygen species production and nadph oxidase 1 expression in human intestinal epithelial cells |
| url | http://dx.doi.org/10.1155/2023/3286330 |
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