MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism
Abstract Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-42511-z |
| _version_ | 1797558221678837760 |
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| author | Julia Dluzewska Wojciech Dziegielewski Maja Szymanska-Lejman Monika Gazecka Ian R. Henderson James D. Higgins Piotr A. Ziolkowski |
| author_facet | Julia Dluzewska Wojciech Dziegielewski Maja Szymanska-Lejman Monika Gazecka Ian R. Henderson James D. Higgins Piotr A. Ziolkowski |
| author_sort | Julia Dluzewska |
| collection | DOAJ |
| description | Abstract Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent manner. To understand how MSH2 regulates crossovers formed by the two pathways, we combined Arabidopsis mutants that elevate non-interfering crossovers with msh2 mutants. We demonstrate that MSH2 blocks non-interfering crossovers at polymorphic loci, which is the opposite effect to interfering crossovers. We also observe MSH2-independent crossover inhibition at highly polymorphic sites. We measure recombination along the chromosome arms in lines differing in patterns of heterozygosity and observe a MSH2-dependent crossover increase at the boundaries between heterozygous and homozygous regions. Here, we show that MSH2 is a master regulator of meiotic DSB repair in Arabidopsis, with antagonistic effects on interfering and non-interfering crossovers, which shapes the crossover landscape in relation to interhomolog polymorphism. |
| first_indexed | 2024-03-10T17:28:15Z |
| format | Article |
| id | doaj.art-44b0467b9e4e4b8b8aebd7c126978097 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:28:15Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-44b0467b9e4e4b8b8aebd7c1269780972023-11-20T10:06:40ZengNature PortfolioNature Communications2041-17232023-10-0114111610.1038/s41467-023-42511-zMSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphismJulia Dluzewska0Wojciech Dziegielewski1Maja Szymanska-Lejman2Monika Gazecka3Ian R. Henderson4James D. Higgins5Piotr A. Ziolkowski6Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityLaboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityLaboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityLaboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityDepartment of Plant Sciences, University of CambridgeDepartment of Genetics and Genome Biology, University of LeicesterLaboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityAbstract Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent manner. To understand how MSH2 regulates crossovers formed by the two pathways, we combined Arabidopsis mutants that elevate non-interfering crossovers with msh2 mutants. We demonstrate that MSH2 blocks non-interfering crossovers at polymorphic loci, which is the opposite effect to interfering crossovers. We also observe MSH2-independent crossover inhibition at highly polymorphic sites. We measure recombination along the chromosome arms in lines differing in patterns of heterozygosity and observe a MSH2-dependent crossover increase at the boundaries between heterozygous and homozygous regions. Here, we show that MSH2 is a master regulator of meiotic DSB repair in Arabidopsis, with antagonistic effects on interfering and non-interfering crossovers, which shapes the crossover landscape in relation to interhomolog polymorphism.https://doi.org/10.1038/s41467-023-42511-z |
| spellingShingle | Julia Dluzewska Wojciech Dziegielewski Maja Szymanska-Lejman Monika Gazecka Ian R. Henderson James D. Higgins Piotr A. Ziolkowski MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism Nature Communications |
| title | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
| title_full | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
| title_fullStr | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
| title_full_unstemmed | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
| title_short | MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism |
| title_sort | msh2 stimulates interfering and inhibits non interfering crossovers in response to genetic polymorphism |
| url | https://doi.org/10.1038/s41467-023-42511-z |
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