Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L.
Chloroplast development is crucial for photosynthesis and plant growth and many factors are involved in its regulation. The regulatory mechanism differs in different green tissues, and previous studies have focused on chloroplasts in leaves. In this study, a mutant with sepal-specific chlorophyll-de...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.850330/full |
| _version_ | 1819104598593372160 |
|---|---|
| author | Wei Zhang Xiaoying Zhou Feng Chen Hongli Zhu Rui Shi Rui Shi Chengming Sun Song Chen Maolong Hu Jiefu Zhang Jiefu Zhang Xiaodong Wang |
| author_facet | Wei Zhang Xiaoying Zhou Feng Chen Hongli Zhu Rui Shi Rui Shi Chengming Sun Song Chen Maolong Hu Jiefu Zhang Jiefu Zhang Xiaodong Wang |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | Chloroplast development is crucial for photosynthesis and plant growth and many factors are involved in its regulation. The regulatory mechanism differs in different green tissues, and previous studies have focused on chloroplasts in leaves. In this study, a mutant with sepal-specific chlorophyll-deficiency was observed in Brassica napus and named as df74. Genetic analysis indicated that the phenotype was controlled by a single recessive nuclear gene. The gene was located on chromosome C08 by bulked-segregant analysis with whole-genome sequencing, which was designated as BnC08.cds. To fine-map the BnC08.cds, a F2 population was created from the cross of df74 and Zhongshuang11 (ZS11). Finally, the BnC08.cds was fine-mapped in the region between the single-nucleotide polymorphism (SNP) markers M5 and M6, corresponding to a 228.72 kb interval of the B. napus “ZS11” genome. Eighteen genes were predicted in the target region, and it was speculated that BnaC08G0442100ZS was the most likely candidate gene based on the results of transcriptome analyses and sequence variation analyses. These results provide a foundation to explore the regulation of chloroplast development in sepals. |
| first_indexed | 2024-12-22T02:08:54Z |
| format | Article |
| id | doaj.art-44b04de3127049c0b072c10d277e4ec7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T02:08:54Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-44b04de3127049c0b072c10d277e4ec72022-12-21T18:42:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.850330850330Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L.Wei Zhang0Xiaoying Zhou1Feng Chen2Hongli Zhu3Rui Shi4Rui Shi5Chengming Sun6Song Chen7Maolong Hu8Jiefu Zhang9Jiefu Zhang10Xiaodong Wang11Key Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaChloroplast development is crucial for photosynthesis and plant growth and many factors are involved in its regulation. The regulatory mechanism differs in different green tissues, and previous studies have focused on chloroplasts in leaves. In this study, a mutant with sepal-specific chlorophyll-deficiency was observed in Brassica napus and named as df74. Genetic analysis indicated that the phenotype was controlled by a single recessive nuclear gene. The gene was located on chromosome C08 by bulked-segregant analysis with whole-genome sequencing, which was designated as BnC08.cds. To fine-map the BnC08.cds, a F2 population was created from the cross of df74 and Zhongshuang11 (ZS11). Finally, the BnC08.cds was fine-mapped in the region between the single-nucleotide polymorphism (SNP) markers M5 and M6, corresponding to a 228.72 kb interval of the B. napus “ZS11” genome. Eighteen genes were predicted in the target region, and it was speculated that BnaC08G0442100ZS was the most likely candidate gene based on the results of transcriptome analyses and sequence variation analyses. These results provide a foundation to explore the regulation of chloroplast development in sepals.https://www.frontiersin.org/articles/10.3389/fpls.2022.850330/fullBrassica napus Lchlorophyll-deficient mutantfine-mappingRNA-sequencingyellow-sepalcandidate gene |
| spellingShingle | Wei Zhang Xiaoying Zhou Feng Chen Hongli Zhu Rui Shi Rui Shi Chengming Sun Song Chen Maolong Hu Jiefu Zhang Jiefu Zhang Xiaodong Wang Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L. Frontiers in Plant Science Brassica napus L chlorophyll-deficient mutant fine-mapping RNA-sequencing yellow-sepal candidate gene |
| title | Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L. |
| title_full | Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L. |
| title_fullStr | Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L. |
| title_full_unstemmed | Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L. |
| title_short | Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L. |
| title_sort | fine mapping and candidate gene analysis of bnc08 cds a recessive gene responsible for sepal specific chlorophyll deficiency in brassica napus l |
| topic | Brassica napus L chlorophyll-deficient mutant fine-mapping RNA-sequencing yellow-sepal candidate gene |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.850330/full |
| work_keys_str_mv | AT weizhang finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT xiaoyingzhou finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT fengchen finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT honglizhu finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT ruishi finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT ruishi finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT chengmingsun finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT songchen finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT maolonghu finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT jiefuzhang finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT jiefuzhang finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl AT xiaodongwang finemappingandcandidategeneanalysisofbnc08cdsarecessivegeneresponsibleforsepalspecificchlorophylldeficiencyinbrassicanapusl |