Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein
Continuous outbreaks of viral diseases in humans facilitates a need for the rapid development of viral test kits and vaccines. These require expression systems to produce a pure and high yield of target viral proteins. We utilized a baculovirus–silkworm expression system to produce the receptor bind...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/6/672 |
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| author | Youpeng Fan Junhong Wei Wei Wang Chunfeng Li Guoqing Pan Timothy Keiffer Jialing Bao Zeyang Zhou |
| author_facet | Youpeng Fan Junhong Wei Wei Wang Chunfeng Li Guoqing Pan Timothy Keiffer Jialing Bao Zeyang Zhou |
| author_sort | Youpeng Fan |
| collection | DOAJ |
| description | Continuous outbreaks of viral diseases in humans facilitates a need for the rapid development of viral test kits and vaccines. These require expression systems to produce a pure and high yield of target viral proteins. We utilized a baculovirus–silkworm expression system to produce the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. First, we had to develop a strategy for constructing a recombinant baculovirus for RBD expression. For this, the coding region of the <i>Bombyx mori</i> cypovirus (BmCPV) polyhedron was assembled with the <i>Bombyx mori</i> nuclear polyhedrosis virus (BmNPV) promoter. We demonstrated that the recombinant baculovirus has the ability to form polyhedrons within host silkworm cells. In addition, the encapsulated BVs are able to infect silkworms by ingestion and induce foreign protein expression. In this way, we utilized this novel system to obtain a high yield of the target foreign protein, the RBD of the SARS-CoV-2 S protein. However, the viral infection rate of our recombinant BV needs to be improved. Our study shed light on developing a highly efficient expression system for the production of antigens and subsequent immunoassays and vaccines. |
| first_indexed | 2024-03-09T22:48:18Z |
| format | Article |
| id | doaj.art-44b0e7bc6a704943a7211e3518438123 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:48:18Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-44b0e7bc6a704943a7211e35184381232023-11-23T18:25:03ZengMDPI AGPathogens2076-08172022-06-0111667210.3390/pathogens11060672Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike ProteinYoupeng Fan0Junhong Wei1Wei Wang2Chunfeng Li3Guoqing Pan4Timothy Keiffer5Jialing Bao6Zeyang Zhou7State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, ChinaDepartment of Microbiology and Immunology, Center for Molecular and Tumor Virology, Feist-Weiller Cancer Center, Louisiana State University, Baton Rouge, LA 71130, USAState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, ChinaContinuous outbreaks of viral diseases in humans facilitates a need for the rapid development of viral test kits and vaccines. These require expression systems to produce a pure and high yield of target viral proteins. We utilized a baculovirus–silkworm expression system to produce the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. First, we had to develop a strategy for constructing a recombinant baculovirus for RBD expression. For this, the coding region of the <i>Bombyx mori</i> cypovirus (BmCPV) polyhedron was assembled with the <i>Bombyx mori</i> nuclear polyhedrosis virus (BmNPV) promoter. We demonstrated that the recombinant baculovirus has the ability to form polyhedrons within host silkworm cells. In addition, the encapsulated BVs are able to infect silkworms by ingestion and induce foreign protein expression. In this way, we utilized this novel system to obtain a high yield of the target foreign protein, the RBD of the SARS-CoV-2 S protein. However, the viral infection rate of our recombinant BV needs to be improved. Our study shed light on developing a highly efficient expression system for the production of antigens and subsequent immunoassays and vaccines.https://www.mdpi.com/2076-0817/11/6/672baculovirusBmNPVBmCPVCOVID-19 spike proteinRBD |
| spellingShingle | Youpeng Fan Junhong Wei Wei Wang Chunfeng Li Guoqing Pan Timothy Keiffer Jialing Bao Zeyang Zhou Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein Pathogens baculovirus BmNPV BmCPV COVID-19 spike protein RBD |
| title | Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein |
| title_full | Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein |
| title_fullStr | Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein |
| title_full_unstemmed | Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein |
| title_short | Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein |
| title_sort | utilization of recombinant baculovirus expression system to produce the rbd domain of sars cov 2 spike protein |
| topic | baculovirus BmNPV BmCPV COVID-19 spike protein RBD |
| url | https://www.mdpi.com/2076-0817/11/6/672 |
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