Seasonal activity-profiles of enzymes involved in cryoprotectant biosynthesis in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae)
The activities of three enzymes involved in polyol biosynthesis (aldose reductase, AR; ketose reductase, KR; and polyol dehydrogenase, PDH) were studied in adult females of the linden bug, Pyrrhocoris apterus, collected from the field during 2005/2006. While the activities of three enzymes were low...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2008-02-01
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| Series: | European Journal of Entomology |
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| Online Access: | https://www.eje.cz/artkey/eje-200801-0020_Seasonal_activity-profiles_of_enzymes_involved_in_cryoprotectant_biosynthesis_in_Pyrrhocoris_apterus_Heteropte.php |
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| author | Michaela TOLLAROVÁ |
| author_facet | Michaela TOLLAROVÁ |
| author_sort | Michaela TOLLAROVÁ |
| collection | DOAJ |
| description | The activities of three enzymes involved in polyol biosynthesis (aldose reductase, AR; ketose reductase, KR; and polyol dehydrogenase, PDH) were studied in adult females of the linden bug, Pyrrhocoris apterus, collected from the field during 2005/2006. While the activities of three enzymes were low in reproductive females, activities greater by one or two orders were seen in reproductively arrested females. AR and KR showed similar seasonal trends in activity. Activities were low during diapause initation and later increased and stabilized during autumnal diapause development. Further increases of AR and KR activities were seen during low temperature quiescence and finally the activities sharply decreased during vernal resumption of direct development. The activity of PDH was relatively high (but fluctuating) during diapause, then decreased in quiescent insects and almost disapeared in reproductively active females. Insects collected in February were subjected to laboratory de-acclimation (exposure to high temperatures) followed by re-acclimation (exposure to low temperatures) which resulted in loss of activity in all three enzymes and no regain. High activities of AR, KR and PDH in reproductively arrested females thus conform well with their previously observed high capacity to synthesize and accumulate polyol cryoprotectants. |
| first_indexed | 2024-12-13T12:03:57Z |
| format | Article |
| id | doaj.art-44b252d424b441a2a41de06b7f3ab151 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-13T12:03:57Z |
| publishDate | 2008-02-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-44b252d424b441a2a41de06b7f3ab1512022-12-21T23:47:01ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292008-02-01105114915210.14411/eje.2008.020eje-200801-0020Seasonal activity-profiles of enzymes involved in cryoprotectant biosynthesis in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae)Michaela TOLLAROVÁ0Institute of Entomology, Biology Centre AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic and Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic; e-mail: michaela.tollarova@seznam.czThe activities of three enzymes involved in polyol biosynthesis (aldose reductase, AR; ketose reductase, KR; and polyol dehydrogenase, PDH) were studied in adult females of the linden bug, Pyrrhocoris apterus, collected from the field during 2005/2006. While the activities of three enzymes were low in reproductive females, activities greater by one or two orders were seen in reproductively arrested females. AR and KR showed similar seasonal trends in activity. Activities were low during diapause initation and later increased and stabilized during autumnal diapause development. Further increases of AR and KR activities were seen during low temperature quiescence and finally the activities sharply decreased during vernal resumption of direct development. The activity of PDH was relatively high (but fluctuating) during diapause, then decreased in quiescent insects and almost disapeared in reproductively active females. Insects collected in February were subjected to laboratory de-acclimation (exposure to high temperatures) followed by re-acclimation (exposure to low temperatures) which resulted in loss of activity in all three enzymes and no regain. High activities of AR, KR and PDH in reproductively arrested females thus conform well with their previously observed high capacity to synthesize and accumulate polyol cryoprotectants.https://www.eje.cz/artkey/eje-200801-0020_Seasonal_activity-profiles_of_enzymes_involved_in_cryoprotectant_biosynthesis_in_Pyrrhocoris_apterus_Heteropte.phpdiapauseoverwinteringmetabolismenzymespolyolsheteropterapyrrhocoris apterus |
| spellingShingle | Michaela TOLLAROVÁ Seasonal activity-profiles of enzymes involved in cryoprotectant biosynthesis in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) European Journal of Entomology diapause overwintering metabolism enzymes polyols heteroptera pyrrhocoris apterus |
| title | Seasonal activity-profiles of enzymes involved in cryoprotectant biosynthesis in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) |
| title_full | Seasonal activity-profiles of enzymes involved in cryoprotectant biosynthesis in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) |
| title_fullStr | Seasonal activity-profiles of enzymes involved in cryoprotectant biosynthesis in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) |
| title_full_unstemmed | Seasonal activity-profiles of enzymes involved in cryoprotectant biosynthesis in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) |
| title_short | Seasonal activity-profiles of enzymes involved in cryoprotectant biosynthesis in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) |
| title_sort | seasonal activity profiles of enzymes involved in cryoprotectant biosynthesis in pyrrhocoris apterus heteroptera pyrrhocoridae |
| topic | diapause overwintering metabolism enzymes polyols heteroptera pyrrhocoris apterus |
| url | https://www.eje.cz/artkey/eje-200801-0020_Seasonal_activity-profiles_of_enzymes_involved_in_cryoprotectant_biosynthesis_in_Pyrrhocoris_apterus_Heteropte.php |
| work_keys_str_mv | AT michaelatollarova seasonalactivityprofilesofenzymesinvolvedincryoprotectantbiosynthesisinpyrrhocorisapterusheteropterapyrrhocoridae |